STUDY ON THE ANTIMICROBIAL ACTIVITY OF the THICK SALVIA OFFICINALIS LEAF EXTRACT IN COMBINATION WITH VARIOUS SUBSTANCES IN ORDER TO MAKE A MEDICINE FOR APPLYING IN GYNECOLOGY

Relevance. Infectious and inflammatory diseases of genitals is one of the factors that deteriorate female reproductive health. The treatment is aimed at eradication of pathogenic flora, resolution of infection and prevention of delayed sequela. Currently natural antimicrobial substances in intravaginal dosage forms has become an alternative for the use of chemical antibacterial topical products. Aim. The purpose of this study was to obtain data on the antimicrobial activity of Salvia officinalis leaf extract of various concentrations and in combination with various substances for further substantiation of the vaginal suppositories’ formulation used in gynecological practice. Materials and methods. The antimicrobial activity of the samples has been studied by the conventional procedure in microbiological practice by agar well diffusion method. Specimens were solutions of Salvia officinalis leaf extract in the concentration from 0.25% to 1.25% (table). Standard mixtures of Salvia officinalis leaf extract with 1% and 0.5% solutions of citric and benzoic acids in various combinations have been also used. Results. The enhancement of antimicrobial activity has been noted by means of increasing the extract concentration in the solution. However, an increase of reactant concentration of more than 1% is impractical as the increase in the antimicrobial effect is insignificant. Taking into account the standard mixtures of Salvia officinalis leaf extract with 1% and 0.5% solutions of citric and benzoic acids in various combinations, it has been found that the sample which includes 1% solutions of Salvia officinalis leaf extract, citric and benzoic acids has the highest possible antimicrobial and antifungal activity. Conclusions. The optimal concentrations of active substances for applying in the formulation of vaginal suppositories in order to achieve the highest possible antimicrobial and antifungal activity have been determined.

Salvia officinalis leaf extract, vaginal suppositories, infectious and inflammatory gynecological diseases