The densitometric quantitation of barbiturates in the whole blood

The aim of the study was the development of quantitation technique of some drugs in whole blood by means of thin-layer chromatography with videodensitometry. The study subject were model samples of whole blood containing barbital, phenobarbital, barbamyl (amobarbital). The identification parameters of drugs by thin-layer chromatography were defined, interferences of blood matrix and other drugs were studied. Limits of detection in blood samples were 600,0 ng/ml (5,0 μg/spot) for barbital, phenobarbital, barbamyl. The chromatogram scanning and processing images calibration models by using videodensitometry computer program were employed for quantitation. The calibration model is described by polynomial (square) regression. The limits of quantitation for different drugs ranges from 1200,0 to 1500,0 ng/ml; accuracy and precision don’t exceed 23%. Conclusion. The quantitation technique of some barbiturtes in whole blood by thin-layer chromatography with videodensitometry was developed. The ranges of quantitation allow to use this technique for forensic chemistry and clinical analitycal toxicology.

barbiturates, thin-layer chromatography, videodensitometry, quantitation