Email this article In-vitro modeling of a biofilm of endoparodontal focus of infection
- Authors: Ginnatulina S.I.1
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Affiliations:
- Samara State Medical University
- Issue: Vol 21, No 1-2 (2021)
- Pages: 64-68
- Section: Clinical Medicine
- URL: https://bakhtiniada.ru/2410-3764/article/view/108835
- DOI: https://doi.org/10.55531/2072-2354.2021.21.1.64-68
- ID: 108835
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Abstract
- The development of the model of endoparodontal lesion in vitro is necessary due to the lack of the method for creating a biofilm of endodonto- and periodontopathogenic bacteria in vitro with the reconstruction of communication ways between the endodontium and the periodontium. We have proposed the method, the technical result of which is the maximal reconstruction of a multi-species mixed bacterial biofilm in tooth tissues in the environment similar to in vivo conditions, with the possibility of processing root canals and periodontal pockets in vitro. The proposed model is effective for the cultivation of obligate-anaerobic and facultative-anaerobic cultures.
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##article.viewOnOriginalSite##About the authors
Sofya I. Ginnatulina
Samara State Medical University
Author for correspondence.
Email: gsi1993@yandex.ru
Full-time postgraduate student, Department of Therapeutic Dentistry
Russian Federation, SamaraReferences
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Supplementary files
Supplementary Files
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Fig. 1. Glass test tube
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Fig. 2. Mixing the impression compound
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Fig. 3. Placement of the tooth into the impression compound
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Fig. 4. Sterilization of models in the autoclave
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Fig. 5. Sample prototype
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Fig. 6. Collection the content of the periodontal pocket and root canal and transporting it in the Cary–Blair transport medium
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Fig. 7. Stages of enoculum creation, isolation of monocultures
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Fig. 8. Colony of Str. intermedius in a Petri dish
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Fig. 9. Colony of Gran. adiacens in a Petri dish
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Fig. 10. Colony of Str. mutans in a Petri dish
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