EFFECTIVENESS OF MOLECULAR-GENETIC DIAGNOSTICS DURING PERTUSSIS INFECTION FOCI EXAMINATION
- 作者: Pimenova A.S.1, Borisova O.Y.1,2, Tsvircun O.V.1, Basov A.S.1, Aleshkin V.A.1, Afanasiev S.S.1, Donskich E.E.2, Pikina A.P.2, Kafarskaya L.I.2, Afanasiev M.S.3, Karaulov A.V.3
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隶属关系:
- G.N. Gabrichevsky Institute of Epidemiology and Microbiology
- The Russian National Research Medical University named after N.I. Pirogov
- Sechenov Moscow State Medical University
- 期: 卷 7, 编号 2 (2017)
- 页面: 162-170
- 栏目: ORIGINAL ARTICLES
- URL: https://bakhtiniada.ru/2220-7619/article/view/118538
- DOI: https://doi.org/10.15789/2220-7619-2017-2-162-170
- ID: 118538
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Purpose: whooping cough diagnostics by molecular-genetic methods.
Materials and methods. Under observation there were 4930 people during the period from 2012 to 2015. All samples were received in 8 schools of Moscow and the Moscow region: in 3 schools had been found children with whooping cough, in other 5 schools – only children with prolonged cough. Whooping cough diagnosis had been given earlier by bacteriological and serological methods. 430 clinical samples were studied by 2 methods: PCR with fluorescent hybridized detection of amplified products and isothermal amplification.
Results. In three of eight schools whooping cough cases at 7 children at the age of 7, 9, 11 and 15 years were revealed earlier. The diagnosis of whooping cough at them was confirmed by means of bacteriological and serological methods. 33 positive DNA samples (7.7%) are revealed. DNA-positive samples are allocated from 18 pupils and 15 employees of schools. In two schools where earlier infection sources were established, 15 DNA-positive samples from which in three cases clinical manifestations were observed are revealed. In those schools where it wasn’t earlier established a source of an infection and examinations conducted it is long the coughing children, 18 DNA-positive samples are revealed, and in two cases clinical manifestations in the form of cough were observed.
Conclusion. Performed research confirmed high efectiveness of molecular-genetic methods during pertussis infection foci examination in schools for infection source identification also amongst long coughing children.
作者简介
A. Pimenova
G.N. Gabrichevsky Institute of Epidemiology and Microbiology
Email: fake@neicon.ru
Junior Researcher, Laboratory of Diagnostics of Diphtheria and Pertussis Infections,
Moscow
俄罗斯联邦O. Borisova
G.N. Gabrichevsky Institute of Epidemiology and Microbiology;The Russian National Research Medical University named after N.I. Pirogov
编辑信件的主要联系方式.
Email: olgborisova@mail.ru
PhD, MD (Medicine), Associate Professor, Head of Laboratory of Diagnostics of Diphtheria and Pertussis Infections, 125212, Moscow, Admiral Makarov str., 10;
Professor of Department of Microbiology and Virology,
Moscow
俄罗斯联邦O. Tsvircun
G.N. Gabrichevsky Institute of Epidemiology and Microbiology
Email: fake@neicon.ru
PhD, MD (Medicine), Head Researcher of Laboratory of Whooping Cough and Measles,
Moscow
俄罗斯联邦A. Basov
G.N. Gabrichevsky Institute of Epidemiology and Microbiology
Email: fake@neicon.ru
Junior Researcher, Laboratory of Whooping Cough and Measles,
Moscow
俄罗斯联邦V. Aleshkin
G.N. Gabrichevsky Institute of Epidemiology and Microbiology
Email: fake@neicon.ru
PhD, MD (Medicine), Professor, Director,
Moscow
俄罗斯联邦S. Afanasiev
G.N. Gabrichevsky Institute of Epidemiology and Microbiology
Email: fake@neicon.ru
PhD, MD (Medicine), Professor, Deputy Director for Biotechnology Department,
Moscow
俄罗斯联邦E. Donskich
The Russian National Research Medical University named after N.I. Pirogov
Email: fake@neicon.ru
PhD (Biology), Associate Professor, Associate Professor of Department of Microbiology and Virology,
Moscow
俄罗斯联邦A. Pikina
The Russian National Research Medical University named after N.I. Pirogov
Email: fake@neicon.ru
Assistant Professor, Department of Microbiology and Virology,
Moscow
俄罗斯联邦L. Kafarskaya
The Russian National Research Medical University named after N.I. Pirogov
Email: fake@neicon.ru
PhD, MD (Medicine), Professor, Head of the Department of Microbiology and Virology,
Moscow
俄罗斯联邦M. Afanasiev
Sechenov Moscow State Medical University
Email: fake@neicon.ru
PhD, MD (Medicine), Professor of the Department of Clinical Allergology and Immunology,
Moscow
俄罗斯联邦A. Karaulov
Sechenov Moscow State Medical University
Email: fake@neicon.ru
RAS Full Member, PhD, MD (Medicine), Professor, Head of the Department of Clinical Allergology and Immunology,
Moscow
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