Interpretation of the Seidel test for open eye injury: Experimental study
- 作者: Fomina N.V.1, Boiko E.V.1,2,3, Terenin V.S.4, Petrosyan Y.M.1,2
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隶属关系:
- North-Western State Medical University named after I.I. Mechnikov
- Saint Petersburg Branch of S.N. Fyodorov Eye Microsurgery
- Saint Petersburg University
- Limited Liability Company Family Medicine Center
- 期: 卷 19, 编号 2 (2024)
- 页面: 73-80
- 栏目: Original study article
- URL: https://bakhtiniada.ru/1993-1859/article/view/275851
- DOI: https://doi.org/10.17816/rpoj626205
- ID: 275851
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详细
AIM: The study aimed to examine the phenomenon of fluorescence during the Seidel test on an experimental model of open eye injury (OEI).
MATERIAL AND METHODS: The experimental study used cadaver pig eyes and was conducted in the Wetlab educational laboratory of the St. Petersburg branch of the S.N. Fedorov National Medical Research Center Interbranch Scientific and Technical Complex “Eye Microsurgery”. After creating a perforated hole, the cornea was stained with fluorescein and observed under a cobalt-blue slit-lamp light. After the test strips were removed, the experiment was timed.
RESULTS: The fluorescence phenomenon was noted immediately after applying the stain to the ocular surface of cadaver eyes with OEI. Fluorescein was dissolved in the flowing intraocular fluid (IOF) and was restrained at the wound edges. Then, the color of the flowing IOF changed. In the experiment, two phases of the flowing IOF color were registered, that is, up to 2.95 s, a bright-green fluid flow was noted. After 2.95 s, the stain washed out, and the main stream of liquid was divided into several streams with varying degrees of staining intensity. At 4.12 s, a transparent stream of escaping liquid was noted in the center and green streams along the edges.
CONCLUSION: The Seidel test consists of two successive phases, namely, the initial appearance of a “bright-green stream” of IOF flowing through the corneal wound, lasting no more than 3 s after the administration of fluorescein; and during phase 2, in the flow center, the fluid became transparent, and bright-green staining remained at the edges. The Seidel test should be performed in compliance with all conditions that ensure the manifestations of fluorescence, always using a cobalt slit-lamp filter, and the result should be evaluated considering the sequence of its phases.
作者简介
Natalya Fomina
North-Western State Medical University named after I.I. Mechnikov
编辑信件的主要联系方式.
Email: natalya.fomina@szgmu.ru
ORCID iD: 0000-0003-0816-2891
SPIN 代码: 4125-2640
MD, Cand. Sci. (Med.), Assistant Professor
俄罗斯联邦, 41, Kirochnaya street, 191015, Saint-PetersburgErnest Boiko
North-Western State Medical University named after I.I. Mechnikov; Saint Petersburg Branch of S.N. Fyodorov Eye Microsurgery; Saint Petersburg University
Email: office@mntk.spb.ru
ORCID iD: 0000-0002-7413-7478
SPIN 代码: 7589-2512
MD, Dr. Sci. (Med.); Professor
俄罗斯联邦, 41, Kirochnaya street, 191015, Saint-Petersburg; Saint-Petersburg; Saint-PetersburgVitaly Terenin
Limited Liability Company Family Medicine Center
Email: vitaly.terenin0702@gmail.com
ORCID iD: 0009-0002-2578-5329
SPIN 代码: 1848-2682
MD, ophthalmologist
俄罗斯联邦, TomskYurij Petrosyan
North-Western State Medical University named after I.I. Mechnikov; Saint Petersburg Branch of S.N. Fyodorov Eye Microsurgery
Email: petrosyan.yurij@yandex.ru
ORCID iD: 0000-0003-4081-0078
SPIN 代码: 7524-8382
MD, ophthalmologist
俄罗斯联邦, 41, Kirochnaya street, 191015, Saint-Petersburg; Saint-Petersburg参考
- Linner E, Friedenwald JS. The appearance time of fluorescein as an index of aqueous flow. Am J Ophthalmol. 1957;44(2):225–229. doi: 10.1016/0002-9394(57)90011-9
- Romanchuk KG. Fluorescein. Physiochemical factors affecting its fluorescence. Surv Ophthalmol. 1982;26(5):269–283. doi: 10.1016/0039-6257(82)90163-1
- Bekker Y. Spectroscopia. Мoscow: Technosphera 2009; 527 p. (In Russ.)
- Pothen AG, Parmar M. Fluorescein. 2023 May 29. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing, 2024. Available from: www.ncbi.nlm.nih.gov/books/NBK555957/
- Lebensohn JE. Fluorescein in ophthalmology. Am J Ophthalmol. 1969;67(2):272–274. doi: 10.1016/0002-9394(69)93161-4
- Seidel E. Weitere Experiementelle Untersuchungen uber die Quelle und den Verlauf der lntraokularen Safstromung. III. Uber den Vorgang der Physiologischen Kammerwasserabsonderung und Seine Pharmakoloeische Beeinflussing. Arch Klin Exp Ophthalmol. 1920; 102:372-382.
- Seidel E. Weitere Experiementelle Untersuchungen uber die Quelle und den Verlauf der lntraokularen Safstromung. VI. Die Filtrationsfahigkeit. eine Wesentliche Eigenschaft der Scleralnarben nach Erfolgreicher Elliotscher Trepanation. Arch Klin Exp Ophthalmol. 1921;104;158-161.
- Stevens S. Ophthalmic practice. Community Eye Health. 2005;18(53):79.
- Gundorova RA, Neroev VV, Kashnikov VV. Eye injury. Moscow: GEOTAR-Media; 2014. 560 p. (In Russ.)
- Chentsova EV, Alekseeva IB, Kulikov AN. Clinical guidelines. Open eye injury: clinical picture, diagnosis, treatment. Association of Ophthalmologists; 2017. Available from: www.avo-portal.ru. (In Russ.)
- Oxford handbook of ophthalmology. Denniston AKO, Murray PI, editors. 4th ed. Oxford: Oxford Medical Handbooks; 2018. doi: 10.1093/med/9780198804550.001.0001
- Practical ophthalmology: A manual for beginning residents. Blomquist P.H., editor. American Academy of Ophthalmology; 2021. Р.134–135.
- Vekshin NL. Fluorescence spectroscopy of biopolymers. Pushchino: Foton-vek; 2014. (In Russ.) EDN: QKRGVT
- Argüeso P, Guzman-Aranguez A, Mantelli F, et al. Association of cell surface mucins with galectin-3 contributes to the ocular surface epithelial barrier. J Biol Chem. 2009;284(34):23037–23045. doi: 10.1074/jbc.M109.033332
- Marignan R. Spectroscopic methods and in particular spectrofluorimetrу: The Quality Control of Medicines: Proceedings of the 35th International Congress of Pharmaceutical Sciences; Dublin, 1975; Elsevier; 1976. doi: 10.1016/B978-0-444-41454-0.50011-8
- Doughty MJ. pH dependent spectral properties of sodium fluorescein ophthalmic solutions revisited. Ophthalmic Physiol Opt. 2010;30(2):167–174. doi: 10.1111/j.1475-1313.2009.00703.x
- Practical fluorescence, theory, methods and techniques. Guilbault GG, editor. New York; 1973. P. 23–28.
- Xu R, Teich W, Frenzel F, Hoffmann K, et al. Optical characterization of sodium fluorescein in vitro and ex vivo. Front Oncol. 2021;10(11):654300. doi: 10.3389/fonc.2021.654300
- Liu X, Liu X, Wang Y, et al. Proteome characterization of glaucoma aqueous humor. Mol Cell Proteomics. 2021;20:100117. doi: 10.1016/j.mcpro.2021.100117
- Rossi M, Di Censo F, Di Censo M, Oum MA. Changes in aqueous humor pH after femtosecond laser-assisted cataract surgery. J Refract Surg. 2015;31(7):462–465. doi: 10.3928/1081597X-20150623-04
- Fischer FH, Wiederholt M. Human precorneal tear film pH measured by microelectrodes. Graefes Arch Clin Exp Ophthalmol. 1982;218(3):168–170. doi: 10.1007/BF02215658
- Wessing A. Fluorescein angiography of the retina. Textbook and atlas. Br J Ophthalmol. 1970;54(2):142.
- Cain W, Sinskey RM. Detection of anterior chamber leakage with Seidel’s test. Arch Ophthalmol. 1981;99(11):2013. doi: 10.1001/archopht.1981.03930020889015
