Study of cultivation conditions of avar carnation (Dianthus awaricus Khar.) in vitro
- 作者: Osmanov R.M.1
-
隶属关系:
- Mountain Botanical Garden is a Separate Division of the Federal State Budgetary Scientific Institution “Dagestan Federal Research Center of the Russian Academy of Sciences” (MounBG – OP FSBSI DFRC RAS)
- 期: 卷 28, 编号 12 (2025)
- 页面: 151-160
- 栏目: Problems of experimental biology and medicine
- URL: https://bakhtiniada.ru/1560-9596/article/view/362647
- DOI: https://doi.org/10.29296/25877313-2025-12-18
- ID: 362647
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详细
Introduction. Dianthus awaricus Khar. is an endemic species of Dagestan flora, belonging to the group of white-flowered species and grown in experimental plots of the Mountain Botanical Garden of the DFRC RAS. This species is promising not only for ornamental gardening, but also due to its potential due to the presence of phenolic compounds and fatty oils in the seeds.
The purpose of the study is to evaluate the cultivation conditions and the possibility of clonal reproduction of Avar carnation in vitro.
Materials and methods. The experimental part of the work was carried out in the research laboratory of plant physiology and biotechnology named after Professor A.G. Yusufov at the biological faculty of the Federal State Budgetary Educational Institution of Higher Education "Dagestan State University". The objects of the study were the seed material of Dianthus awaricus, represented by the samples "Tsudakhar", "Gunib" and "Agvali". Using standard biotechnological approaches, various types of explants (nodal, apical and hypocotyl) were cultivated on Murashige-Skoog (MS) nutrient media supplemented with phytohormones: indole-3-butyric acid (IMA) and benzylaminopurine (BAP).
Results. The combination of 1 mg/l BAP and 0.1 mg/l IMC showed the highest efficiency for nodal and apical explants of all the studied samples. The analysis of variance confirmed the significant effect of the type of explants on most of the studied traits (p < 0.05), so the factors "explants" and "phytohormones" had a statistically significant effect on the growth traits. The correlation analysis demonstrated the presence of strong positive relationships between the morphological parameters (height, number of roots, leaves and shoots). A high degree of correlation was established between the number of roots and the number of leaves (up to r = 0.99).
Conclusions. The study of the in vitro cultivation conditions of Dianthus awaricus was conducted for the first time, which demonstrated the possibility of effective application of the tissue culture method for its propagation. Optimal conditions for explant cultivation for several introduced samples of Dianthus awaricus were proposed, allowing to significantly increase the reproduction rate and preserve the genetic potential of the endemic. The optimal nutrient media for propagation are combinations of phytohormones 2.5 mg/l BAP and 0.5 mg/l, as well as 1 mg/l BAP and 0.1 mg/l IMC (nodal and apical explants), IMC at a concentration of 0.5 mg/l (hypocotyl explants).
作者简介
R. Osmanov
Mountain Botanical Garden is a Separate Division of the Federal State Budgetary Scientific Institution “Dagestan Federal Research Center of the Russian Academy of Sciences” (MounBG – OP FSBSI DFRC RAS)
编辑信件的主要联系方式.
Email: ru.osmanov@mail.ru
ORCID iD: 0000-0002-4857-6354
SPIN 代码: 7086-9430
Ph.D. (Biol), Research Scientist, Laboratory of Flora and Plant Resources
俄罗斯联邦, 75 M. Yaragsky str., Makhachkala, 367030参考
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