Effect of Akkermansia muciniphila-based probiotic on small intestinal morphology and gene expression and behavioral characteristics of mice under induced systemic inflammation
- 作者: Pogorelova S.V.1, Chirkin E.A.1, Shutikov V.A.2, Mikhailov E.V.2, Syromyatnikov M.Y.1
-
隶属关系:
- Voronezh State University of Engineering Technologies
- All-Russian Veterinary Research Institute of Pathology, Pharmacology and Therapy
- 期: 卷 28, 编号 12 (2025)
- 页面: 126-134
- 栏目: Problems of experimental biology and medicine
- URL: https://bakhtiniada.ru/1560-9596/article/view/362644
- DOI: https://doi.org/10.29296/25877313-2025-12-15
- ID: 362644
如何引用文章
开放存取
##reader.subscriptionAccessGranted##
订阅存取
详细
Introduction. The microbiome plays an important role in the normal functioning of the body, an imbalance in its composition leads to various diseases. Currently, many studies are being conducted on the bacterium Akkermansia muciniphila, which is a new generation probiotic that has a beneficial effect on the composition of the microbiome. However, the mechanism of the effect of the bacterium on inflammatory processes occurring in the body and induced by various drugs has not been fully studied.
The aim of the study was to determine the effect of A. muciniphila on serum biochemical parameters, as well as behavioral and physiological characteristics of mice during lipopolysaccharide (LPS)-induced systemic inflammation.
Material and methods. The following methods were used in the study: physiological test "Open field", biochemical analysis of blood serum using the automatic analyzer "VitaLine 200", histological examination of the small intestine was carried out using hematoxylin and eosin staining, gene expression was assessed by RT-PCR combined with reverse transcription. Statistical processing was performed in the R environment (Version 4.1.1) and Statistica 12, using the Spearman correlation coefficient, the Kruskal–Wallis test and the Bonferroni correction, one-way variance test (ANOVA) using the Tukey post-hoc test.
Results. A. muciniphila inhibited the inflammatory process induced by LPS injections and, as a result, led to an increase in research activity. The restoration of the small intestine structure was observed. Also, against the background of the probiotic effect, normalization of the biochemical parameters of the blood serum was revealed.
Conclusion. With the combined administration of LPS and A. muciniphila, antioxidant protection (GCLC) and intestinal barrier function (ZO-1) were enhanced, thus, the probiotic demonstrates therapeutic potential in the course of the inflammatory process.
作者简介
S. Pogorelova
Voronezh State University of Engineering Technologies
编辑信件的主要联系方式.
Email: zubkowa.sweta@gmail.com
ORCID iD: 0009-0002-7924-8027
SPIN 代码: 4269-6171
Junior Research Scientist, Laboratory of Metagenomics and Food Biotechnology
俄罗斯联邦, 19 Revolution Avenue, Voronezh, 394036E. Chirkin
Voronezh State University of Engineering Technologies
Email: chirkin@bio.vsu.ru
ORCID iD: 0009-0000-0021-6408
SPIN 代码: 2757-7601
Student, Technician, Laboratory of Metagenomics and Food Biotechnology
俄罗斯联邦, 19 Revolution Avenue, Voronezh, 394036V. Shutikov
All-Russian Veterinary Research Institute of Pathology, Pharmacology and Therapy
Email: shutikov.02@yandex.ru
ORCID iD: 0009-0004-2018-2662
SPIN 代码: 1167-3528
Junior Research Scientist, Laboratory of Innovative Recombinant Proteomics Preparations
俄罗斯联邦, 114b Lomonosov str., Voronezh, 394087E. Mikhailov
All-Russian Veterinary Research Institute of Pathology, Pharmacology and Therapy
Email: voronezh81@rambler.ru
ORCID iD: 0000-0001-5457-1325
SPIN 代码: 9506-5052
Ph.D. (Vet.), Head of the Department of Experimental Pharmacology and Functioning of Living Systems
俄罗斯联邦, 114b Lomonosov str., Voronezh, 394087M. Syromyatnikov
Voronezh State University of Engineering Technologies
Email: syromyatnikov@bio.vsu.ru
ORCID iD: 0000-0001-9028-0613
SPIN 代码: 7321-3642
Ph.D. (Biol.), Leading Research Scientist, Laboratory of Metagenomics and Food Biotechnology, Associate Professor, Department of Genetics, Cytology and Bioengineering
俄罗斯联邦, 19 Revolution Avenue, Voronezh, 394036参考
- Lifshits K., Zakharova I.N., Dmitrieva Yu.A. The influence of the intestinal microbiome in norm and pathology on human health. Medical Council. 2017; 1: 155–159. (In Russ.). doi: 10.21518/2079-701X-2017-1-155-159.
- Kaibysheva V.O., Zharova M.E., Filimendikova K.Yu. et al. Diseases associated with disturbances in the composition of intestinal microbiota. Doctor.Ru. 2021; 20(4): 40–45. (In Russ.). doi: 10.31550/1727-2378-2021-20-4-40-45.
- Bisengaliev R.M., Sadykov R.S., Akbatyrova E.T. Probiotics and prebiotics as the basis of functional nutrition. Young scientist. 2016; 8(112): 185–188. (In Russ.).
- Gao F., Cheng C., Li R. et al. The role of Akkermansia muciniphila in maintaining health: a bibliometric study. Front Med (Lausanne). 2025; 3(12): 1484656. doi: 10.3389/fmed.2025.1484656.
- Lakshmanan A.P., Murugesan S., Al Khodor S. et al. A. The potential impact of a probiotic: Akkermansia muciniphila in the regulation of blood pressure-the current facts and evidence. J Transl Med. 2022; 20(1): 430. doi: 10.1186/s12967-022-03631-0.
- Zakharova I.N., Berezhnaya I.V., Dubovets N.F. et al. Mysterious Akkermansia muciniphila. What do we know about it? A review. Pediatrics. Consilium Medicum. 2023; 1: 74–80. (In Russ.). doi: 10.26442/26586630.2023.1.202190.
- Ahn J.S., Kim S., Han E.J. et al. Increasing spatial working memory in mice with Akkermansia muciniphila. Commun Biol. 2025; 8(1): 546. doi: 10.1038/s42003-025-07975-3.
- Qi Y., Yu L., Tian F. et al. A. muciniphila Supplementation in Mice during Pregnancy and Lactation Affects the Maternal Intestinal Microenvironment. Nutrients. 2022; 14(2): 390. doi: 10.3390/nu14020390.
- Gryaznova M., Burakova I., Smirnova Y. et al. Effect of Probiotic Bacteria on the Gut Microbiome of Mice with Lipopolysaccharide-Induced Inflammation. Microorganisms. 2024; 12(7): 1341. doi: 10.3390/microorganisms12071341.
- Nautiyal K.M., Tritschler L., Ahmari S.E. A Lack of Serotonin 1B Autoreceptors Results in Decreased Anxiety and Depression-Related Behaviors. Neuropsychopharmacology. 2016; 41(12): 2941–2950. doi: 10.1038/npp.2016.109.
- Seibenhener M.L., Wooten M.C. Use of the Open Field Maze to measure locomotor and anxiety-like behavior in mice. J Vis Exp. 2015; (96): e52434. doi: 10.3791/52434.
- Wu W., Kaicen W., Bian X. et al. Akkermansia muciniphila alleviates high-fat-diet-related metabolic-associated fatty liver disease by modulating gut microbiota and bile acids. Microb Biotechnol. 2023; 16(10): 1924–1939. doi: 10.1111/1751-7915.14293.
- Malkova O.G. The role of lipid metabolism disorders in severe sepsis. Ural Medical Journal. 2017; 3(147): 157–163. (In Russ.).
- Fomina A.A., Malinin M.L., Konnova S.A. et al. Effect of lipopolysaccharide Azospirillum lipoferum sp59b on the activity of key metabolic enzymes in mice. Modern Problems of Science and Education. 2015; 5: 638. (In Russ.).
- Williams J.M., Duckworth C.A., Watson A.J. et al. A mouse model of pathological small intestinal epithelial cell apoptosis and shedding induced by systemic administration of lipopolysaccharide. Dis Model Mech. 2013; 6(6): 1388–1399. doi: 10.1242/dmm.013284.
- He K.Y., Lei X.Y., Wu D.H. et al. Akkermansia muciniphila protects the intestine from irradiation-induced injury by secretion of propionic acid. Gut Microbes. 2023; 15(2): 2293312. doi: 10.1080/19490976.2023.2293312.
- Valova Ya.V., Kutlina T.G., Mukhammadieva G.F. et al. Analysis of GCLC and GSTT gene expression in acute toxic hepatitis in rats. Occupational Medicine and Human Ecology. 2019; 2: 75–79. (In Russ.). doi: 10.24411/2411-3794-2019-10025.
- Simanenkov V.I., Maev I.V., Tkacheva O.N. et al. Increased epithelial permeability syndrome in clinical practice. Multidisciplinary national consensus. Cardiovascular Therapy and Prevention. 2021; 20(1): 121–278. (In Russ.). doi: 10.15829/1728-8800-2021-2758.
补充文件
