Comparative characteristics of the quantitative content of polyprenols in substances derived from Ginkgo biloba L. and Picea abies L.
- Authors: Antipina А.А.1, Popov N.S.2, Baranov M.S.3,4, Myasnyanko I.N.3,4, Savintsev S.V.3,4, Balabanyan V.Y.3
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Affiliations:
- M.V. Lomonosov Moscow State University
- Tver State Medical University, Ministry of Health of the Russian Federation
- Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
- Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
- Issue: Vol 27, No 8 (2024)
- Pages: 13-21
- Section: Pharmaceutical chemistry
- URL: https://bakhtiniada.ru/1560-9596/article/view/263101
- DOI: https://doi.org/10.29296/25877313-2024-08-02
- ID: 263101
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Abstract
Introduction. Polyprenols are known as a class of natural long-chain isoprenoid alcohols, which are fat-soluble antioxidants and natural bioregulators that directly participate in the synthesis of glycoproteins of cell membranes. Their hepatoprotective activity is proven, as well as other types of their pharmacological effects are known, which is the reason of significant interest in these substances as a promising medicinal product. The main sources of polyprenols are coniferous trees (various types of spruce (Picea spp.), fir (Abies spp.), pine (Pinus spp.)), as well as Ginkgo biloba L.). Preparative isolation of polyprenols from plants is carried out by extraction based on the difference in solubility in two or more organic solvents.
Aim. Identification and comparative quantitative assessment of polyprenols in substances derived from Ginkgo biloba L. and Picea abies L. according to the author’s method in Savintsev’s laboratory.
Material and methods. Identification of polyprenols in substances was carried out using proton nuclear magnetic resonance (NMR) spectroscopy, Fourier-transform infrared spectroscopy (FTIR), mass spectrometry (MS), and high-performance liquid chromatography (HPLC). The quantitative content of polyprenols was determined using HPLC-MS, a triple quadrupole mass spectrometer was used as a detector.
Results. Polyprenols in substances were identified by 1H NMR spectroscopy, IR spectroscopy, mass spectrometry and high-performance liquid chromatography. Using HPLC-MS, the quantitative content of C70 – C100 polyprenols in samples of Ginkgo biloba L. and Picea abies L. was found to be 76% and 95% on average, respectively.
Conclusions. The predominance of shorter polyprenols in the substance of Picea abies L. compared to the substance of Ginkgo biloba L. can be suggested based on the results of comparative characteristics of the chromatographic peaks expression and the results of
1H NMR spectroscopy. The regulatory documentation can be prepared based on the results of this study for subsequent state registration of pharmaceutical substances and medicines of polyprenols derived from Ginkgo biloba L. and Picea abies L.
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##article.viewOnOriginalSite##About the authors
А. А. Antipina
M.V. Lomonosov Moscow State University
Author for correspondence.
Email: antipina_aa@rambler.ru
ORCID iD: 0000-0002-8970-3495
Post-graduate Student
Russian Federation, 1, Leninskie Gory str., Moscow, 119991N. S. Popov
Tver State Medical University, Ministry of Health of the Russian Federation
Email: ns.popov@mail.ru
ORCID iD: 0000-0002-1792-7414
Ph.D. (Pharm.), Associate Professor
Russian Federation, 4, Sovetskaya str., Tver, 170100M. S. Baranov
Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: baranovmikes@gmail.com
ORCID iD: 0000-0002-9339-7603
D.Sc. (Chem.)
Russian Federation, 1 bldg. 6 Ostrovityanova str., Moscow, 117997; 16/10, Miklukho-Maklaya str., GSP-7, Moscow, 117997I. N. Myasnyanko
Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: ivan.n.myasnyanko@gmail.com
ORCID iD: 0000-0002-2168-3555
Ph.D. (Chem.)
Russian Federation, 1 bldg. 6 Ostrovityanova str., Moscow, 117997; 16/10, Miklukho-Maklaya str., GSP-7, Moscow, 117997S. V. Savintsev
Email: ssv53@mail.ru
ORCID iD: 0009-0000-5639-5548
Individual entrepreneur
Russian FederationV. Yu. Balabanyan
Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation
Email: bal.pharm@mail.ru
ORCID iD: 0000-0002-5744-7060
D.Sc. (Pharm.)
Russian Federation, 1 bldg. 6 Ostrovityanova str., Moscow, 117997References
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