α1-Thymosin, α2-interferon, and the LKEKK syntetic peptide inhibit the binding of the B subunit of the cholera toxin to intestinal epithelial cell membranes
- Авторы: Navolotskaya E.V.1, Sadovnikov V.B.1, Zinchenko D.V.1, Vladimirov V.I.1,2, Zolotarev Y.A.3
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Учреждения:
- Pushchino Branch, Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- Pushchino State Natural Science Institute
- Institute of Molecular Genetics
- Выпуск: Том 43, № 6 (2017)
- Страницы: 673-677
- Раздел: Article
- URL: https://bakhtiniada.ru/1068-1620/article/view/228738
- DOI: https://doi.org/10.1134/S1068162017060115
- ID: 228738
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Аннотация
The 125I-labeled B-subunit of the cholera toxin ([125I]CT-B, specific activity of 98 Ci/mmol) was prepared. This subunit was shown to be bound to the membranes which were isolated from epithelial cells of a mucous tunic of the rat thin intestine with high affinity (Kd = 3.7 nM). The binding of the labeled protein was inhibited by the unlabeled α2-interferon (IFN-α2), α1-thymosin, (TM-α1), and the LKEKK synthetic peptide corresponding to the 16–20 sequence of TM-α1 and the 131–135 sequence of human IFN-α2 (Ki 1.0, 1.5, and 2.0 nM, respectively), whereas the KKEKL unlabeled synthetic peptide did not inhibit the binding (Ki > 100 μМ). The LKEKK peptide and CT-B were shown to dose-dependently increase an activity of the soluble guanylate cyclase (sGC) in the concentration range from 10 to 1000 nM. Thus, the binding of TM- α1, IFN-α2, and the LKEKK peptide to the CT-B receptor on a surface of the epithelial cells of the mucous tunic of the rat thin intestine resulted in an increase in the intracellular level of cGMP.
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Об авторах
E. Navolotskaya
Pushchino Branch, Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Автор, ответственный за переписку.
Email: navolotskaya@bibch.ru
Россия, Pushchino, Moscow oblast, 142290
V. Sadovnikov
Pushchino Branch, Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: navolotskaya@bibch.ru
Россия, Pushchino, Moscow oblast, 142290
D. Zinchenko
Pushchino Branch, Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: navolotskaya@bibch.ru
Россия, Pushchino, Moscow oblast, 142290
V. Vladimirov
Pushchino Branch, Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry; Pushchino State Natural Science Institute
Email: navolotskaya@bibch.ru
Россия, Pushchino, Moscow oblast, 142290; Pushchino, Moscow oblast, 142290
Y. Zolotarev
Institute of Molecular Genetics
Email: navolotskaya@bibch.ru
Россия, Moscow, 123182
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