Email this article Novel 5-Alkylcarboxamide-2'-Deoxyuridine-5'-Triphosphates for Enzymatic Synthesis of Highly Modified DNA
- Authors: Spitsyn M.A.1,2, Guseinov T.O.1,2, Miftahov R.A.1,2, Zasedateleva O.A.1, Lisitsa A.V.3, Radko S.P.2,3, Zasedatelev A.S.1, Timofeev E.N.1, Chudinov A.V.1,2, Vasiliskov V.A.1, Lapa S.A.1, Kuznetsova V.E.1, Surzhikov S.A.1, Shershov V.E.1
-
Affiliations:
- Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
- IBMС-EcoBioPharm
- Orekhovich Research Institute of Biomedical Chemistry, Russian Academy of Sciences
- Issue: Vol 45, No 3 (2019)
- Pages: 221-223
- Section: Letter to Editor
- URL: https://bakhtiniada.ru/1068-1620/article/view/229198
- DOI: https://doi.org/10.1134/S1068162019030063
- ID: 229198
Cite item
Open Access
Access granted
Subscription Access
Abstract
The synthesis of deoxyuridine triphosphate derivatives with alkyl groups of various chain length attached to the pyrimidine base by the trans-alkene linker was performed. The efficiency of the incorporation of modified nucleotides during the synthesis of DNA with a high degree of modification by the primer extension reaction with Taq DNA polymerase was studied.
About the authors
M. A. Spitsyn
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences; IBMС-EcoBioPharm
Email: chud@eimb.ru
Russian Federation, Moscow, 119991; Moscow, 119121
T. O. Guseinov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences; IBMС-EcoBioPharm
Email: chud@eimb.ru
Russian Federation, Moscow, 119991; Moscow, 119121
R. A. Miftahov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences; IBMС-EcoBioPharm
Email: chud@eimb.ru
Russian Federation, Moscow, 119991; Moscow, 119121
O. A. Zasedateleva
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: chud@eimb.ru
Russian Federation, Moscow, 119991
A. V. Lisitsa
Orekhovich Research Institute of Biomedical Chemistry, Russian Academy of Sciences
Email: chud@eimb.ru
Russian Federation, Moscow, 119121
S. P. Radko
IBMС-EcoBioPharm; Orekhovich Research Institute of Biomedical Chemistry, Russian Academy of Sciences
Email: chud@eimb.ru
Russian Federation, Moscow, 119121; Moscow, 119121
A. S. Zasedatelev
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: chud@eimb.ru
Russian Federation, Moscow, 119991
E. N. Timofeev
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: chud@eimb.ru
Russian Federation, Moscow, 119991
A. V. Chudinov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences; IBMС-EcoBioPharm
Author for correspondence.
Email: chud@eimb.ru
Russian Federation, Moscow, 119991; Moscow, 119121
V. A. Vasiliskov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: chud@eimb.ru
Russian Federation, Moscow, 119991
S. A. Lapa
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: chud@eimb.ru
Russian Federation, Moscow, 119991
V. E. Kuznetsova
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: chud@eimb.ru
Russian Federation, Moscow, 119991
S. A. Surzhikov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: chud@eimb.ru
Russian Federation, Moscow, 119991
V. E. Shershov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: chud@eimb.ru
Russian Federation, Moscow, 119991
Supplementary files
