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Estimation of the degree of conjugation of oligosaccharide haptens to bovine serum albumin in the course of the squarate procedure using gel permeation HPLC


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Abstract

An approach to estimate the degree of conjugation of oligosaccharide haptens to bovine serum albumin by the squarate procedure was proposed. The approach includes gel permeation HPLC in combination with the multichannel detection.

About the authors

D. E. Tsvetkov

N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences

Email: nen@ioc.ac.ru
Russian Federation, 47 Leninsky prosp., Moscow, 119991

E. V. Sukhova

N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences

Email: nen@ioc.ac.ru
Russian Federation, 47 Leninsky prosp., Moscow, 119991

A. A. Karelin

N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences

Email: nen@ioc.ac.ru
Russian Federation, 47 Leninsky prosp., Moscow, 119991

Yu. E. Tsvetkov

N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences

Email: nen@ioc.ac.ru
Russian Federation, 47 Leninsky prosp., Moscow, 119991

N. E. Nifantiev

N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences

Author for correspondence.
Email: nen@ioc.ac.ru
Russian Federation, 47 Leninsky prosp., Moscow, 119991

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