Email this article Atomic force microscopy of swelling and hardening of intact erythrocytes fixed on substrate
- Authors: Khalisov M.M.1,2, Timoshchuk K.I.2, Ankudinov A.V.2,3, Timoshenko T.E.1
-
Affiliations:
- Pavlov Institute of Physiology
- ITMO University
- Ioffe Physicotechnical Institute
- Issue: Vol 62, No 2 (2017)
- Pages: 310-313
- Section: Biomedical Physics
- URL: https://bakhtiniada.ru/1063-7842/article/view/198978
- DOI: https://doi.org/10.1134/S1063784217020116
- ID: 198978
Cite item
Open Access
Access granted
Subscription Access
Abstract
Peak force measurements with the aid of atomic force microscopy are used to quantitatively map nanomechanical properties of intact erythrocytes of rats under conditions that are close to physiological conditions. Erythrocytes that are immobilized on the substrate preliminary processed using poly-L-lysine predominantly exhibit plane shape. However, cells may also exhibit stepwise transformation to semispherical objects with an increase in volume and hardening. Possible reasons for such transformations are discussed.
About the authors
M. M. Khalisov
Pavlov Institute of Physiology; ITMO University
Email: alexander.ankudinov@mail.ioffe.ru
Russian Federation, nab. Makarova 6, St. Petersburg, 199034; Kronverkskii pr. 49, St. Petersburg, 197101
K. I. Timoshchuk
ITMO University
Email: alexander.ankudinov@mail.ioffe.ru
Russian Federation, Kronverkskii pr. 49, St. Petersburg, 197101
A. V. Ankudinov
ITMO University; Ioffe Physicotechnical Institute
Author for correspondence.
Email: alexander.ankudinov@mail.ioffe.ru
Russian Federation, Kronverkskii pr. 49, St. Petersburg, 197101; Politekhnicheskaya ul. 26, St. Petersburg, 194021
T. E. Timoshenko
Pavlov Institute of Physiology
Email: alexander.ankudinov@mail.ioffe.ru
Russian Federation, nab. Makarova 6, St. Petersburg, 199034
Supplementary files
