STEARYLAMINE ACTIVATES RAPID AND ROS-INDEPENDENT FORMATION OF NEUTROPHIL EXTRACELLULAR TRAPS (NETOSIS)
- Authors: Lotosh N.Y.1, Selischeva A.A.1,2, Vorobjeva N.V.3
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Affiliations:
- National Research Center «Kurchatov Institute»
- Lomonosov Moscow State University
- Lomonosov Moscow State University, Faculty of Biology
- Issue: Vol 22, No 2-2 (2019)
- Pages: 855-857
- Section: ORIGINAL ARTICLES
- URL: https://bakhtiniada.ru/1028-7221/article/view/119762
- DOI: https://doi.org/10.31857/S102872210006650-0
- ID: 119762
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Abstract
Using confocal fluorescence microscopy it was shown that stearylamine (SA) at concentration of 0.2 mg/ml causes the neutrophil extracellular traps formation. In comparison with classic NET activator, the phorbol ether of myristate acetate (PMA), SA activates this process much faster, in 30‒60 min, while under PMA stimulation it occurs in 2‒3 hours. Using luminol-dependent chemiluminescence, we showed that SA does not activate NADPH oxidase. SA dose-dependently suppresses the oxidative burst stimulated with PMA, zymosan, or latex. The direct evidence that NADPH oxidase is not activated under the SA action was obtained by using neutrophils isolated from the blood of patients with chronic granulomatous disease (CGD). It is obvious from our results, that SA-induced NET formation does not require the participation of reactive oxygen species and proceeds according to a nonclassical mechanism.
About the authors
N. Y. Lotosh
National Research Center «Kurchatov Institute»
Author for correspondence.
Email: natalotosh@gmail.com
Ph.D., Research Associate, Department of Biotechnology and Bioenergy,
Moscow
Russian FederationA. A. Selischeva
National Research Center «Kurchatov Institute»;Lomonosov Moscow State University
Email: fake@neicon.ru
Doctor of Chemical Sciences, Research Engineer;
Leading Research Associate, Faculty of Biology,
Moscow
Russian FederationN. V. Vorobjeva
Lomonosov Moscow State University, Faculty of Biology
Email: fake@neicon.ru
Ph.D., Senior Research Associate,
Moscow
Russian FederationReferences
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