PHENOTYPING OF ANTIGEN-SPECIFIC CYTOTOXIC T-LYMPHOCYTES BY THE METHOD OF MULTICOLOR FLOW CYTOMETRY
- Authors: Kuznetsova M.S.1, Lopatnikova J.A.1, Sennikov S.V.1,2
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Affiliations:
- Research Institute of Fundamental and Clinical Immunology
- Novosibirsk State University
- Issue: Vol 22, No 2-2 (2019)
- Pages: 837-839
- Section: ORIGINAL ARTICLES
- URL: https://bakhtiniada.ru/1028-7221/article/view/119709
- DOI: https://doi.org/10.31857/S102872210006680-3
- ID: 119709
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Abstract
Modern methods of multicolor flow cytometry in combination with MHC multimer technologies allow not only to identify and isolate cytotoxic T-lymphocyte (CTL) populations, but also to conduct phenotypic research on the level of differentiation, functional features of these cells, and even to investigate the distribution of memory T-cell subsets inside populations specific to particular epitopes of tumor antigens. In the present study, we assessed the level of differentiation and the content of memory T-cell subpopulations in cytotoxic T-lymphocyte populations specific for HER2/neu antigen epitopes (HER2) using the MHC-multimers antigen-specific CD8+ T-cell staining method and multicolor flow cytometry. The resulting HER2-specific T cells to a large extent (about 40‒50%) have the TSCM phenotype, a population capable of the most pronounced antitumor immune response due to a combination of effector properties with self-sustainability.
About the authors
M. S. Kuznetsova
Research Institute of Fundamental and Clinical Immunology
Author for correspondence.
junior researcher of the Laboratory of Molecular Immunology,
Novosibirsk
Russian FederationJ. A. Lopatnikova
Research Institute of Fundamental and Clinical Immunology
PhD, senior researcher of the Laboratory of Molecular Immunology,
Novosibirsk
Russian FederationS. V. Sennikov
Research Institute of Fundamental and Clinical Immunology;Novosibirsk State University
Email: sennikovsv@gmail.com
PhD, MD, professor, head of the Laboratory of Molecular Immunology,
Novosibirsk
Russian FederationReferences
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- Kuznetsova M., Lopatnikova J., Khantakova J., Maksyutov R., Maksyutov A., Sennikov S. Generation of populations of antigen-specific cytotoxic T cells using DCs transfected with DNA construct encoding HER2/neu tumor antigen epitopes. // BMC Immunol 2017, 18, doi: 10.1186/s12865-017-0219-7.
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