Post-Irradiation Changes in the Number of γH2AX and pATM Protein Foci in Human Mesenchymal Stem Cells Irradiated with 14.1 MeV Neutrons
- Authors: Korneva S.A.1, Chigasova A.K.1,2,3, Osipov A.A.2, Ignatov M.A.1,2, Vorobyova N.Y.1,2, Saburov V.O.4, Kazakov E.I.4, Koryakin S.N.4, Fedotov Y.A.1,2, Bushmanov A.Y.1, Osipov A.N.1,2
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Affiliations:
- A.I. Burnazyan Federal Medical Biophysical Center
- N.N. Semenov Federal Research Center for Chemical Physics
- Institute of Biochemical Physics
- A.F. Tsyb Medical Radiological Research Center
- Issue: Vol 70, No 3 (2025)
- Pages: 11-15
- Section: Radiation Biology
- URL: https://bakhtiniada.ru/1024-6177/article/view/361488
- DOI: https://doi.org/10.33266/1024-6177-2025-70-3-11-15
- ID: 361488
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Abstract
Purpose: Comparative analysis of dose-response dependences and kinetics of post-radiation changes in the number of γH2AX and pATM protein foci in human mesenchymal stem cells (MSCs) exposed to 14.1 MeV neutrons and cobalt-60 gamma-radiation.
Material and methods: The study used a primary culture of human MSCs obtained from the collection of BioloT LLC (Russia). The cells were irradiated using a neutron generator NG-14 (VNIIA, Russia), which provided neutron fluxes with an energy of 14.1 MeV, and a gamma-therapeutic device ROKUS-AM (JSC Ravenstvo, Russia; cobalt-60, dose rate 0.5 Gy/min) at doses of 0.1, 0.25 and 0.5 Gy. For quantitative assessment of γH2AX and pATM foci, immunocytochemical staining was done using antibodies to γH2AX and pATM, respectively. Statistical significance was assessed using analysis of variance (ANOVA).
Results: It was shown that the kinetics of post-radiation changes in the number of γH2AX foci in cells irradiated with neutrons is slower than after gamma irradiation. 24 h after irradiation with neutrons, ~ 62 % of γH2AX foci and ~ 52 % of pATM foci were recorded from their number 0.5 h after irradiation. These values were statistically significantly (p < 0.001) higher than the proportions of residual foci calculated after exposure to gamma-radiation: ~ 16 % and 6 %, respectively. The results obtained indicate that the proportion of complex, difficult-to-repair DNA damage in cells irradiated with neutrons is significantly higher than with gamma-radiation.
Keywords
About the authors
S. A. Korneva
A.I. Burnazyan Federal Medical Biophysical Center
Email: andreyan.radbio@gmail.com
Moscow, Russia
A. K. Chigasova
A.I. Burnazyan Federal Medical Biophysical Center; N.N. Semenov Federal Research Center for Chemical Physics; Institute of Biochemical Physics
Email: andreyan.radbio@gmail.com
Moscow, Russia; Moscow, Russia; Moscow, Russia
A. A. Osipov
N.N. Semenov Federal Research Center for Chemical Physics
Email: andreyan.radbio@gmail.com
Moscow, Russia
M. A. Ignatov
A.I. Burnazyan Federal Medical Biophysical Center; N.N. Semenov Federal Research Center for Chemical Physics
Email: andreyan.radbio@gmail.com
Moscow, Russia; Moscow, Russia
N. Yu. Vorobyova
A.I. Burnazyan Federal Medical Biophysical Center; N.N. Semenov Federal Research Center for Chemical Physics
Email: andreyan.radbio@gmail.com
Moscow, Russia; Moscow, Russia
V. O. Saburov
A.F. Tsyb Medical Radiological Research Center
Email: andreyan.radbio@gmail.com
Obninsk, Russia
E. I. Kazakov
A.F. Tsyb Medical Radiological Research Center
Email: andreyan.radbio@gmail.com
Obninsk, Russia
S. N. Koryakin
A.F. Tsyb Medical Radiological Research Center
Email: andreyan.radbio@gmail.com
Obninsk, Russia
Yu. A. Fedotov
A.I. Burnazyan Federal Medical Biophysical Center; N.N. Semenov Federal Research Center for Chemical Physics
Email: andreyan.radbio@gmail.com
Moscow, Russia; Moscow, Russia
A. Yu. Bushmanov
A.I. Burnazyan Federal Medical Biophysical Center
Email: andreyan.radbio@gmail.com
Moscow, Russia
A. N. Osipov
A.I. Burnazyan Federal Medical Biophysical Center; N.N. Semenov Federal Research Center for Chemical Physics
Email: andreyan.radbio@gmail.com
Moscow, Russia; Moscow, Russia
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