Combined effect of laser radiation and ozone on purulent wound healing in experiment and clinic

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Combined influence of ultra-violet, helium-neon lasers and ozone on the healing of purulent wound was studied in experiment (30 rats) and clinic (30 patients). The results were evaluated by the rate of wound healing, cytologic and histologic pictures as well as the changes in blood content. The experimental study showed that combined application of ultra-violet laser and ozone 1.4 times reduced the time of purulent wound epithelization and additional irradiation by helium-neon laser performed in the second stage of wound process accelerated proliferation and differentiation of the newly formed integument. Each of those physical-chemical factors (ozone, ultra-violet and heliumneon lasers) exerted positive effect on a purulent wound and their consecutive application allowed to achieve the best results in 79.4% of cases.

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E. Nazarov

Ryazan State Medical University. acad. I.P. Pavlova

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俄罗斯联邦, Ryazan

V. Papkov

Ryazan State Medical University. acad. I.P. Pavlova

Email: info@eco-vector.com
俄罗斯联邦, Ryazan

I. Fokin

Ryazan State Medical University. acad. I.P. Pavlova

Email: info@eco-vector.com
俄罗斯联邦, Ryazan

参考

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2. Fig. 1. Dynamics of the area of wounds in experimental animals depending on the type of therapeutic effect.

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3. Fig. 2. Dynamics of the cellular composition of wound exudate in experimental animals depending on the type of therapeutic effect.

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4. Fig. 3. Fibrinous-leukocyte exudate at the bottom of the wound defect. Areas of horizontally oriented fibroblasts in the deep sections of the wound bottom (control series of experiments). Here and in fig. 4-6: staining with hematoxylin and eosin. SW. 120. Fig. 4. Differentiation of the regenerated epithelium into layers. Absence of hair follicles in the dermis (III series of experiments). Fig. 5. Epithelialization of the wound defect with differentiation of layers. Regeneration of hair follicles in the dermis (IV series of experiments). Fig. 6. Completed differentiation of the epidermis and hair follicles (V series of experiments).

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5. Fig. 7. Dynamics of blood parameters in patient A. Diagnosis: laceration of the middle third of the right shoulder (ozonation of the wound was performed).

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6. Fig. 8. Dynamics of blood parameters in patient S. Diagnosis: laceration of the upper third of the left leg (ozonation was performed with irradiation of the wound with an ultraviolet and helium-neon laser).

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