Antiviral activity of the dihydroquercetin during the Coxsackievirus В4 replication in vitro
- Authors: Galochkina A.V.1, Zarubaev V.V.1, Kiselev O.I.1, Babkin V.A.2, Ostroukhova L.A.2
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Affiliations:
- Research Institute of Influenza
- A.E. Favorsky Irkutsk Institute of Chemistry, Siberian Branch, Russian Academy of Sciences
- Issue: Vol 61, No 1 (2016)
- Pages: 27-31
- Section: ORIGINAL RESEARCHES
- URL: https://bakhtiniada.ru/0507-4088/article/view/118039
- DOI: https://doi.org/10.18821/0507-4088-2016-61-1-27-31
- ID: 118039
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Abstract
A study of the antiviral activity of antioxidants against viral infections is believed to be essential for creating complex antiviral agents. Dihydroquercetin is considered as the most active antioxidant extracted from larix gmelinii. In this work, we present results of experiments of the antiviral properties of dihydroquercetin against a member of the family picornaviridae – Coxsackievirus B4 in vitro. We have estimated that dihydroquercetin reduces viral titers at 100 µg/ml concentration as compared with control of virus. We have shown using the plaque assay that cpe of virus is reduced in the presence of dihydroquercetin at 100 µg/ml. Study of the phase of viral life cycle, in which dihydroquercetin acted, demonstrated that the highest efficacy of the antiviral therapy was reached at early stages of virus reproduction (1-3 hours post infection). These results show that dihydroquercetin has antiviral property against Coxsackievirus B4. This drug and other antioxidants can be tested as inhibitors of viral replication.
About the authors
A. V. Galochkina
Research Institute of Influenza
Author for correspondence.
Email: nastyagalochkina@yandex.ru
Anastasiya Galochkina, research assistant
197376, St. Petersburg
Russian FederationV. V. Zarubaev
Research Institute of Influenza197376, St. Petersburg Russian Federation
O. I. Kiselev
Research Institute of Influenza197376, St. Petersburg Russian Federation
V. A. Babkin
A.E. Favorsky Irkutsk Institute of Chemistry, Siberian Branch, Russian Academy of Sciences664033, Irkutsk Russian Federation
L. A. Ostroukhova
A.E. Favorsky Irkutsk Institute of Chemistry, Siberian Branch, Russian Academy of Sciences664033, Irkutsk Russian Federation
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