Molecular and genetic characteristics of the multicomponent flavi-like Kindia tick virus (Flaviviridae) found in ixodes ticks on the territory of the Republic of Guinea
- 作者: Kartashov M.Y.1, Gladysheva A.V.1, Naidenova E.V.2, Zakharov K.S.2, Shvalov А.N.1, Krivosheina E.I.1, Senichkina A.M.2, Bah M.B.3, Ternovoi V.A.1, Boumbaly S.3, Loktev V.B.1
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隶属关系:
- State Research Center of Virology and Biotechnology «Vector»
- Russian Research Anti-Plague Institute «Microbe»
- Research Institute of Applied Biology of Guinea
- 期: 卷 67, 编号 6 (2022)
- 页面: 487-495
- 栏目: ORIGINAL RESEARCH
- URL: https://bakhtiniada.ru/0507-4088/article/view/125755
- DOI: https://doi.org/10.36233/0507-4088-145
- ID: 125755
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详细
Introduction. Ixodes ticks are vectors for pathogens of many infectious diseases. Recently, during the study of Rhipicephalus geigyi ticks collected from livestock in the Republic of Guinea, a new multicomponent flavi-like RNA virus, called Kindia tick virus (KITV), was discovered with an unusual mechanism for the implementation of genetic information.
The aim of the work is to detect and study the genetic diversity of KITV in ixodes ticks collected in the territory of the Kindia province of the Republic of Guinea.
Material and methods. In 2021, 324 specimens of ticks of the species Amblyomma variegatum, Rh. geigyi, Rh. annulatus, Rh. decoloratus, Rh. senegalensis were collected from cattle. The detection of viral RNA was carried out in individual samples of ticks by RT-PCR, followed by the determination of the nucleotide sequence and phylogenetic analysis.
Results and discussion. KITV detection rates in ticks of the species Rh. geigyi was 12.2%, Rh. annulatus – 4.4%, Rh. decoloratus – 3.3%. However, the KITV genetic material has not been identified in Am. variegatum ticks, which are one of the dominant species in West Africa. For all virus isolates, a partial nucleotide sequences of each of the four viral segments (GenBank, OK345271–OK345306) were determined. The phylogenetic analysis showed a high level of identity (98.5–99.8%) for each of the four segments of the viral genome with those previously found in the Republic of Guinea. The obtained KITV isolates are most genetically close to Mogiana tick virus, which was previously detected in South America in Rh. microplus ticks and significantly differed from other multicomponent viruses circulating in Europe and Asia, including the Russian Federation.
Conclusion. KITV genetic material was found in three species of ixodid ticks collected from livestock in a number of prefectures of the Republic of Guinea. The infection rate in ticks was 3.3–12.2%. The continuation of research in this direction remains relevant.
作者简介
Mikhail Kartashov
State Research Center of Virology and Biotechnology «Vector»
Email: mikkartash@yandex.ru
ORCID iD: 0000-0002-7857-6822
PhD, MD (Biology), Senior Researcher, Department of Flavivirus Infections
俄罗斯联邦, 630559, Koltsovo, Novosibirsk regionAnastasia Gladysheva
State Research Center of Virology and Biotechnology «Vector»
Email: mikkartash@yandex.ru
ORCID iD: 0000-0002-7396-3954
Junior Researcher, Department of Flavivirus Infections
俄罗斯联邦, 630559, Koltsovo, Novosibirsk regionEkaterina Naidenova
Russian Research Anti-Plague Institute «Microbe»
Email: katim2003@mail.ru
ORCID iD: 0000-0001-6474-3696
PhD, MD (Biology), Senior Researcher, Laboratory of Virology, Department of Infectious Diseases Diagnostics
俄罗斯联邦, 410005, SaratovKirill Zakharov
Russian Research Anti-Plague Institute «Microbe»
Email: mikkartash@yandex.ru
ORCID iD: 0000-0002-4726-309X
PhD, MD (Biology), Senior Researcher
俄罗斯联邦, 410005, SaratovАlexander Shvalov
State Research Center of Virology and Biotechnology «Vector»
Email: mikkartash@yandex.ru
ORCID iD: 0000-0001-6890-1575
PhD (Physics and Mathematics), Senior Researcher
俄罗斯联邦, 630559, Koltsovo, Novosibirsk regionEkaterina Krivosheina
State Research Center of Virology and Biotechnology «Vector»
Email: katr962@mail.ru
ORCID iD: 0000-0001-5181-0415
Junior Researcher, Department of Flavivirus Infections
俄罗斯联邦, 630559, Koltsovo, Novosibirsk regionAislu Senichkina
Russian Research Anti-Plague Institute «Microbe»
Email: mikkartash@yandex.ru
PhD, (Biology), Senior Researcher, Department of Infectious Diseases Diagnostics
俄罗斯联邦, 410005, SaratovMamadou Bah
Research Institute of Applied Biology of Guinea
Email: mikkartash@yandex.ru
ORCID iD: 0000-0002-4565-269X
postgraduate student
几内亚, KindiaVladimir Ternovoi
State Research Center of Virology and Biotechnology «Vector»
Email: tern@vector.nsc.ru
ORCID iD: 0000-0003-1275-171X
PhD, MD (Biology), head of the laboratory Department of Flavivirus Infections
俄罗斯联邦, 630559, Koltsovo, Novosibirsk regionSanaba Boumbaly
Research Institute of Applied Biology of Guinea
Email: drboumbaly@yahoo.fr
ORCID iD: 0000-0002-4506-6033
PhD, chief graduate school Research Institute of Applied Biology
几内亚, KindiaValery Loktev
State Research Center of Virology and Biotechnology «Vector»
编辑信件的主要联系方式.
Email: loktev@vector.nsc.ru
ORCID iD: 0000-0002-0229-321X
Doctor of Biology, Head of Department of Flavivirus Infections
俄罗斯联邦, 630559, Koltsovo, Novosibirsk region参考
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