Production of the polyclonal enterovirus antibodies of chicken (IgY) and its evaluation as alternative to the rabbit enterovirus neutralizing sera
- 作者: Kozlov V.G.1, Ivanov A.P.1, Ivanova O.E.1, Wargin V.V.2
-
隶属关系:
- Federal State Enterprise for Manufacture of Bacterial and Viral Preparations, Shumakov Institute of Poliomyelitis and Viral Encephalitides
- M.P. Shumakov Institute of Poliomyelitis and Viral Encephalitides
- 期: 卷 60, 编号 1 (2015)
- 页面: 31-34
- 栏目: ORIGINAL RESEARCH
- URL: https://bakhtiniada.ru/0507-4088/article/view/118058
- ID: 118058
如何引用文章
全文:
详细
Experimental data show the usefulness of the Leghorn chicken as a producer of the enterovirus neutralizing antibodies (IgY). The resulting serum is not inferior to the specific activity of the commercial rabbit enterovirus diagnostic sera (EDS) in the neutralization reaction. The IgY have lower backgrounds than mammalian IgG and do not cause toxic effect to cell culture. Compared with the conventional manufacturing method EDS IgY, preparation process is much more effective: the number of serum producers is significantly lower, whereas the yield of the product is higher. Reduction of the volume of the immunogens, immunization cycle, and number of injections is also an advantage of this manufacturing method.
作者简介
V. Kozlov
Federal State Enterprise for Manufacture of Bacterial and Viral Preparations, Shumakov Institute of Poliomyelitis and Viral Encephalitides
编辑信件的主要联系方式.
Email: vgkozlov@mail.ru
Vitaliy Kozlov, MD, PhD
142782, Moscow
俄罗斯联邦A. Ivanov
Federal State Enterprise for Manufacture of Bacterial and Viral Preparations, Shumakov Institute of Poliomyelitis and Viral Encephalitides
Email: fake@neicon.ru
142782, Moscow 俄罗斯联邦
O. Ivanova
Federal State Enterprise for Manufacture of Bacterial and Viral Preparations, Shumakov Institute of Poliomyelitis and Viral Encephalitides
Email: fake@neicon.ru
142782, Moscow 俄罗斯联邦
V. Wargin
M.P. Shumakov Institute of Poliomyelitis and Viral Encephalitides
Email: fake@neicon.ru
142782, Moscow 俄罗斯联邦
参考
- Иванова О.Е., Еремеева Т.П., Лукашов А.Н., Байкова О.Ю., Морозова Н.С., Мустафина А.Н. Наблюдение за циркуляцией неполиомиелитных энтеровирусов в Российской Федерации в 1999– 2007 гг. Медицинская вирусология. Тр. ИПВЭ им. М.П. Чумакова РАМН. 2008; ХХV: 11–22.
- Лукашов А.Н., Иванова О.Е., Худякова Л.В. Социальноэкономическая значимость энтеровирусной инфекции и ее роль в структуре инфекционной патологии в мире. Журнал микробиологии, эпидемиологии и иммунологии. 2010; 5: 113–20.
- Рекомендации по эпидемиологическому надзору за энтеровирусами для поддержки программы ликвидации полиомиелита. Женева: ВОЗ; 2005.
- Schade R., Calzado E.G., Sarmiento R., Chacana P.A., Parankiewicz-Asplund J., Terzolo H.R. Chicken egg yolk antibodies (IgYtechnology): a review of progress in production and use in research and human and veterinary medicine. ALTA. 2005; 33: 129–54.
- Russell W.M.S., Burch R.L. The Principles of Humane Experimental Technique. 1959. London, Methuen & Co LTD: reprinted 1992 by UFAW, South Mimms, UK.
- Akita E.M., Nakai S. Comparison of four purification methods for the production of immunoglobulins from eggs laid by hens immunized with an enterotoxigenic E. coli strain. J. Immunol. Meth. 1993; 160: 207–14.
- Horse serum for typing of enteroviruses. National institute of public health and the environment (RIVM), Bilthoven, NL.
- Козлов В.Г., Викторова Е.Г., Набатников П.А. Цитотоксические свойства кроличьих энтеровирусных диагностических сывороток. Особенности и локализация. Вопросы вирусологии. 2009; 1: 22–7.
- Liou J.F., Chang C.W., Tailiu J.J., Yu C.K., Lei H.Y., Chen L.R., Tai. C. Passive protection effect of chicken egg yolk immunoglobulins on enterovirus 71 infected mice. Vaccine. 2010; 28: 8189–96.
- Каркищенко Н.Н. Руководство по лабораторным животным и альтернативным моделям в биологических исследованиях. М.; 2010.
补充文件
