Мақаланы E-mail арқылы жіберу Derivation of Induced Pluripotent Stem Cells Line (RCPCMi009-A-1) with Knockout of the UBE2A Gene by CRISPR/Cas9 Genome Editing
- Авторлар: Khomyakova E.A.1, Fedorenko A.V.1,2, Surdina A.V.1, Volovikov E.A.1,3, Belikova L.D.1,3, Zerkalenkova E.A.4, Lagarkova M.A.1,5, Bogomazova A.N.1,3,5
-
Мекемелер:
- Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine
- Skolkovo Institute of Science and Technology
- Research Institute of Medical Genetics, Tomsk National Research Medical Center of the Russian Academy of Scieces
- Rogachev Federal Scientific and Clinical Centre of Pediatric Hematology, Oncology and Immunology
- Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Lopukhin Federal Research and Clinical Center of Physical and Chemical Medicine
- Шығарылым: Том 54, № 6 (2023)
- Беттер: 405-414
- Бөлім: Collection of pluripotent stem cell lines
- URL: https://bakhtiniada.ru/0475-1450/article/view/232037
- DOI: https://doi.org/10.31857/S0475145023060046
- EDN: https://elibrary.ru/HKXTMY
- ID: 232037
Дәйексөз келтіру
Ашық рұқсат
Рұқсат берілді
Тек жазылушылар үшін
Аннотация
The deletions and mutations in the UBE2A gene cause X-linked mental retardation syndrome of Nascimento type first described in 2006 (Nascimento et al., 2006). To study the role of the UBE2A gene in neurodevelopment, we generated a human iPSC line with knockout of the UBE2A gene (RCPCMi009-A-1) using genome editing CRISPR/Cas9 technology. The knockout of the UBE2A gene was confirmed by Western blotting. The pluripotent state of the RCPCMi009-A-1 iPSCs line was confirmed by typical stem cell morphology, normal male karyotype maintenance, expression of pluripotency markers and the ability to differentiate into three germ layers.
Авторлар туралы
E. Khomyakova
Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine
Хат алмасуға жауапты Автор.
Email: kate.hom@gmail.com
Russia, 119435, Moscow
A. Fedorenko
Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine; Skolkovo Institute of Science and Technology
Email: kate.hom@gmail.com
Russia, 119435, Moscow; Russia, 121205, Moscow
A. Surdina
Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine
Email: kate.hom@gmail.com
Russia, 119435, Moscow
E. Volovikov
Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine; Research Institute of Medical Genetics, Tomsk National Research Medical Center of the Russian Academy of Scieces
Email: kate.hom@gmail.com
Russia, 119435, Moscow; Russia, 634050, Tomsk
L. Belikova
Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine; Research Institute of Medical Genetics, Tomsk National Research Medical Center of the Russian Academy of Scieces
Email: kate.hom@gmail.com
Russia, 119435, Moscow; Russia, 634050, Tomsk
E. Zerkalenkova
Rogachev Federal Scientific and Clinical Centre of Pediatric Hematology, Oncology and Immunology
Email: kate.hom@gmail.com
Russia, 117997, Moscow
M. Lagarkova
Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine; Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Lopukhin Federal Research and Clinical Center of Physical and Chemical Medicine
Email: kate.hom@gmail.com
Russia, 119435, Moscow; Russia, 119435, Moscow
A. Bogomazova
Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine; Research Institute of Medical Genetics, Tomsk National Research Medical Center of the Russian Academy of Scieces; Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Lopukhin Federal Research and Clinical Center of Physical and Chemical Medicine
Email: kate.hom@gmail.com
Russia, 119435, Moscow; Russia, 634050, Tomsk; Russia, 119435, Moscow
Әдебиет тізімі
- Bogomazova A.N., Lagarkova M.A., Tskhovrebova L.V. et al. Error-prone nonhomologous end joining repair operates in human pluripotent stem cells during late G2 // Aging (Albany NY). 2011. V. 3. № 6. P. 584.
- Concordet J.P., Haeussler M. CRISPOR: intuitive guide selection for CRISPR/Cas9 genome editing experiments and screens // Nucleic Acids Research. 2018. V. 46. № W1. P. W242–W245.
- Czepulkowski B., Bhatt B., Rooney D. Malignancy and acquired abnormalities. Vol. 2. Oxford University Press. New York. 1992. V. 2. P. 1–25.
- Dover J., Schneider J., Tawiah-Boateng M.A. et al. Methylation of histone H3 by COMPASS requires ubiquitination of histone H2B by Rad6 // J. Biological Chemistry. 2002. V. 277. № 32. P. 28368–28371.
- Hibbert R.G., Huang A., Boelens R. et al. E3 ligase Rad18 promotes monoubiquitination rather than ubiquitin chain formation by E2 enzyme Rad6 // Proceedings of the National Academy of Sciences. 2011. V. 108. № 14. P. 5590–5595.
- Holmqvist S., Lehtonen Š., Chumarina M. et al. Creation of a library of induced pluripotent stem cells from Parkinsonian patients // npj Parkinson’s Disease. 2016. V. 2. № 1. P. 1–10.
- Nascimento R.M., Otto P.A., de Brouwer A.P. et al. UBE2A, which encodes a ubiquitin-conjugating enzyme, is mutated in a novel X-linked mental retardation syndrome // The American J. Human Genetics. 2006. V. 79. № 3. P. 549–555.
- Pliatsika V., Rigoutsos I. “Off-Spotter”: very fast and exhaustive enumeration of genomic lookalikes for designing CRISPR/Cas guide RNAs // Biology Direct. 2015. V. 10. P. 1–10.
- Van Kuppeveld F.J., Van der Logt J.T., Angulo A.F. et al. Genus-and species-specific identification of mycoplasmas by 16S rRNA amplification // Applied and Environmental Microbiology. 1992. V. 58. № 8. P. 2606–2615.
- Wojcik F., Dann G.P., Beh L.Y. et al. Functional crosstalk between histone H2B ubiquitylation and H2A modifications and variants // Nature Communications. 2018. V. 9. № 1. P 1–11.
Қосымша файлдар
