The adoptive transfer of regulatory B lymphocytes prevents severe damage to lung tissues during respiratory infection with the influenza A/H1N1 virus

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Abstract

The aim of the study is to investigate the role of regulatory B lymphocytes in the regulation of the inflammatory process in the lungs against the background of influenza virus infection (A/H1N1/WSN/1933).

Materials and methods. On the day after intranasal infection with influenza A/H1N1/WSN/1933 virus, CBA/N mice received intravenous B cells from CBA mice: Breg (induced in vitro by activators, high content of IL-10+ regulatory B cells), Bcontr (incubated in vitro without activators, IL-10 content+ minimal regulatory B cells), BPerC (isolated abdominal B cells, without in vitro incubation). On the 4th day after infection, histological changes in the lungs, the number of IgM and IgG antibody producers in the lungs and spleen, as well as the viral load in the lungs were evaluated.

Results. Intranasal infection of CBA/N mice with influenza A/H1N1/WSN/1933 virus was accompanied by pronounced morphological changes in lung tissue detected on day 4, including tissue structural disorders and cellular infiltration. The adoptive transfer of Breg the day after infection was associated with a decrease in the severity of histological signs of lung damage compared with the group without transfer. The transfer of Bcontr and BPerC was also accompanied by a decrease in the detected tissue damage, but the effect was less pronounced than with Breg transfer. In all groups with CBA B cell transfer, there was an increase in the number of IgM and IgG antibody producers in the spleen and lungs. At the same time, there were no differences in the indicator of viral load in the lungs between the compared groups.

Conclusion. The revealed prevention of severe lung tissue damage in CBA/N mice infected with influenza A/H1N1/WSN/1933 virus during the adoptive transfer of in vitro-induced regulatory B cells from CBA mice is attributable to a combination of increased immune response due to the transferred cells and the regulatory activity of IL-10+ regulatory B cells.

About the authors

Ilya N. Dyakov

I.I. Mechnikov Research Institute of Vaccines and Sera

Author for correspondence.
Email: dyakov.instmech@mail.ru
ORCID iD: 0000-0001-5384-9866

Cand. Sci. (Biol.), leading researcher, Head, Laboratory of biosynthesis of immunoglobulins, Immunology and allergology department, I. Mechnikov Research Institute of Vaccines and Sera; researcher, Laboratory of bacterial genetics, Department of medical microbiology, National Research Center for Epidemiology and Microbiology named after N.F. Gamaleya

Russian Federation, Moscow

Irina N. Chernyshova

I.I. Mechnikov Research Institute of Vaccines and Sera

Email: irina.n.chernyshova@gmail.com
ORCID iD: 0000-0001-5053-2433

Cand. Sci. (Med.), senior researcher, Laboratory of biosynthesis of immunoglobulins, Immunology and allergology department

Russian Federation, Moscow

Marina V. Gavrilova

I.I. Mechnikov Research Institute of Vaccines and Sera

Email: gavrilovamv@gmail.com
ORCID iD: 0000-0002-6936-2486

Cand. Sci. (Biol.), researcher, Laboratory of biosynthesis of immunoglobulins, Immunology and allergology department, I. Mechnikov Research Institute of Vaccines and Sera; researcher, Laboratory of bacterial genetics, Department of medical microbiology, National Research Center for Epidemiology and Microbiology named after N.F. Gamaleya

Russian Federation, Moscow

Kristina K. Bushkova

I.I. Mechnikov Research Institute of Vaccines and Sera

Email: christina_bushkova@mail.ru
ORCID iD: 0000-0002-4757-0751

researcher, Laboratory of biosynthesis of immunoglobulins, Immunology and allergology department

Russian Federation, Moscow

Artyom A. Rtishchev

I.I. Mechnikov Research Institute of Vaccines and Sera

Email: rtishchevartyom@gmail.com
ORCID iD: 0000-0002-4212-5093

researcher, Laboratory of genetics of RNA-containing viruses, Department of virology named after O.G. Andzhaparidze

Russian Federation, Moscow

Natalia E. Abayeva

I.I. Mechnikov Research Institute of Vaccines and Sera

Email: fabaeva.nata@list.ru
ORCID iD: 0000-0003-3984-959X

researcher, Laboratory of biosynthesis of immunoglobulins, Immunology and allergology department

Russian Federation, Moscow

Stanislav G. Markushin

I.I. Mechnikov Research Institute of Vaccines and Sera

Email: s.g.markushin@rambler.ru
ORCID iD: 0000-0003-0994-5337

Dr. Sci. (Med.), Head, Laboratory of genetics of RNA-containing viruses, Department of virology named after O.G. Andzhaparidze

Russian Federation, Moscow

Dmitry A. Khochenkov

N.N. Blokhin National Medical Research Center

Email: khochenkov@gmail.com
ORCID iD: 0000-0002-5694-3492

Cand. Sci. (Biol.), Head, Laboratory of biomarkers and mechanisms of tumor angiogenesis

Russian Federation, Moscow

Irina D. Bulgakova

I.I. Mechnikov Research Institute of Vaccines and Sera; Sechenov University

Email: bulgakova_i_d@staff.sechenov.ru
ORCID iD: 0000-0002-2629-9616

assistant, Microbiology, virology and immunology department named after Academician A.A. Vorobyev, Institute of Public Health named after F.F. Erisman, Sechenov University; junior researcher, Laboratory of molecular immunology, I. Mechnikov Research Institute of Vaccines and Sera

Russian Federation, Moscow; Moscow

Nadezhda A. Snegireva

I.I. Mechnikov Research Institute of Vaccines and Sera

Email: snegireva.nadezda@gmail.com
ORCID iD: 0000-0002-5399-3224

researcher, Laboratory of biosynthesis of immunoglobulins, Immunology and allergology department

Russian Federation, Moscow

Oxana A. Svitich

I.I. Mechnikov Research Institute of Vaccines and Sera

Email: svitichoa@yandex.ru
ORCID iD: 0000-0003-1757-8389

Dr. Sci. (Med.), Professor, Full Member of the Russian Academy of Sciences, Director

Russian Federation, Moscow

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Copyright (c) 2025 Dyakov I.N., Chernyshova I.N., Gavrilova M.V., Bushkova K.K., Rtishchev A.A., Abayeva N.E., Markushin S.G., Khochenkov D.A., Bulgakova I.D., Snegireva N.A., Svitich O.A.

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