Development of a method for the quantitative determination of flavonoids in dill herb (Anethum graveolentis herba)
- Авторлар: Bourakba S.1, Marakhova A.I.1, Zhilkina V.Y.1, Bokov D.O.2,3
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Мекемелер:
- Russian Peoples' Friendship University (RUDN)
- I.M. Sechenov First Moscow State Medical University (Sechenov University)
- Federal Research Center for Nutrition and Biotechnology
- Шығарылым: Том 72, № 8 (2023)
- Беттер: 45-49
- Бөлім: Technology of medicines
- URL: https://bakhtiniada.ru/0367-3014/article/view/249753
- DOI: https://doi.org/10.29296/25419218-2023-08-07
- ID: 249753
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Аннотация
Introduction. The dill herb, collected before flowering, contains a complex of various biologically active compounds (BACs) and can act as an independent medicinal plant material. There is data confirming the activity of the aerial part of dill against Agrobacterium radiobacter pv. Tumefaciens, Erwinia carotovora, Pseudomonas fluorescens, Pseudomonas wisteria. The antimicrobial properties of the herb dill may be associated with the presence of phenolic compounds, including flavonoids, in the chemical composition, so the quantitative determination of the amount of these biologically active compounds is relevant.
Purpose of the work: to develop a method for quantitative determination of the amount of flavonoids in dill herb (Anethum graveolentis herba).
Material and methods. The object of study was air-dried dill herb (Anethum graveolens L.), harvested before flowering. Quantitative determination of the amount of flavonoids was carried out by spectrophotometric method after complexation reaction with aluminum chloride on a Lambda-950 spectrophotometer, Perkin Elmer, USA.
Results and discussion. As a result of the study, it was shown that to determine the amount of flavonoids in aromatic dill herb harvested before flowering, the following conditions are optimal: crushing of raw materials to a particle size of 1 mm, mass of raw materials about 2 g, extraction with 80% ethyl alcohol in a volume of 100 ml when heated for 50 min; the ratio of the volume of the extract and 2% aluminum chloride solution is 1:1, the complexation reaction time is 30 minutes.
Conclusion. A method has been developed for determining the amount of flavonoids in terms of rutin and absolutely dry raw materials in dill herb (Anethum graveolentis herba).
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##article.viewOnOriginalSite##Авторлар туралы
Safa Bourakba
Russian Peoples' Friendship University (RUDN)
Хат алмасуға жауапты Автор.
Email: bourakba_s@pfur.ru
ORCID iD: 0009-0009-4421-5373
PhD Student, Institute of Biochemical Technology and Nanotechnology
Ресей, st. Miklouho-Maklaya, 6, Moscow, 117198Anna Marakhova
Russian Peoples' Friendship University (RUDN)
Email: agentcat85@mail.ru
ORCID iD: 0000-0003-4442-9266
Dr. Pharm. Sci., Professor, Institute of Biochemical Technology and Nanotechnology
Ресей, st. Miklouho-Maklaya, 6, Moscow, 117198Vera Zhilkina
Russian Peoples' Friendship University (RUDN)
Email: zhylkina_vyu@pfur.ru
ORCID iD: 0000-0001-7916-5913
Candidate of Pharmaceutical Sciences, Associate Professor of the Institute of Biochemical Technology
Ресей, st. Miklouho-Maklaya, 6, Moscow, 117198Dmitry Bokov
I.M. Sechenov First Moscow State Medical University (Sechenov University); Federal Research Center for Nutrition and Biotechnology
Email: bokov_d_o@staff.sechenov.ru
ORCID iD: 0000-0003-2968-2466
Candidate of Pharmaceutical Sciences, Associate Professor, Associate Professor of the Department of Pharmaceutical Natural Sciences of the Institute of Pharmacy named after A.P. Nelyubin; researcher at the Laboratory of Food Chemistry
Ресей, st. Trubetskaya, 8, building 2, Moscow, 119991; Ustinsky proezd, 2/14, Moscow, 109240Әдебиет тізімі
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