Measurement of metabolites of polycyclic aromatic hydrocarbons in fish bile for comparative assessment of coastal water pollution

Abstract

In the laboratory experiment and in fish caught in conditionally clean and polluted areas, the presence of metabolites of polycyclic aromatic hydrocarbons in bile by methods of conventional and synchronous spectrofluorimetry was determined. The study showed that this approach is well suited for the detection of metabolites of polycyclic aromatic hydrocarbons with 2–3, 4, and 5–6 aromatic rings. If the content of metabolites of polycyclic aromatic hydrocarbons with 4 and 5–6 rings in bile is low, the method of synchronous spectrofluorimetry is less sensitive than conventional spectrofluorimetry. Both methods of monitoring are simple and fast and reflect the real picture of water pollution.