A new sampling format for the diagnostics of bovine infectious diseases in dried blood spots by ELISA


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The detection of antibodies to bovine infectious diseases (enzootic bovine leucosis, viral diarrhea, and infectious rhinotracheitis) is performed by ELISA in dry whole blood samples obtained on porous membranes using a new sampling format. Several commercial test systems are used to diagnose the infections. The obtained data are compared to the results of the analysis of liquid samples of blood serum. The results of the analysis of dried and liquid samples are in full concordance. The new sampling format makes it possible to avoid using a cold chain for the transportation of samples to diagnostic laboratories and to simplify the storage of these samples.

作者简介

N. Saushkin

Department of Chemistry

编辑信件的主要联系方式.
Email: sushk_90@mail.ru
俄罗斯联邦, Moscow, 119991

J. Samsonova

Department of Chemistry; National University of Science and Technology MISiS

Email: sushk_90@mail.ru
俄罗斯联邦, Moscow, 119991; Moscow, 119049

A. Osipov

Department of Chemistry; National University of Science and Technology MISiS

Email: sushk_90@mail.ru
俄罗斯联邦, Moscow, 119991; Moscow, 119049

C. Kondakov

Department of Chemistry; National University of Science and Technology MISiS

Email: sushk_90@mail.ru
俄罗斯联邦, Moscow, 119991; Moscow, 119049

M. Efimova

Federal Center for Toxicological

Email: sushk_90@mail.ru
俄罗斯联邦, Kazan, 420075

A. Chernov

Federal Center for Toxicological

Email: sushk_90@mail.ru
俄罗斯联邦, Kazan, 420075

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