Отправить статью по E-mail The Development of a Multiparametric Immunochromatographic Test System for the Analysis of Cardiomarkers
- Авторы: Andreeva I.P.1, Yakovleva E.A.1, Grigorenko V.G.1, Osipov A.P.1,2
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Учреждения:
- Department of Chemistry, Moscow State University
- National Research Technological University MISiS
- Выпуск: Том 74, № 6 (2019)
- Страницы: 315-321
- Раздел: Article
- URL: https://bakhtiniada.ru/0027-1314/article/view/164068
- DOI: https://doi.org/10.3103/S002713141906004X
- ID: 164068
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Аннотация
Abstract—An immunochromatographic test system for the combined analysis of two cardiomarkers, such as fatty acid binding protein (FABP) and troponin I (TnI), in serum and whole blood is developed. The main stages of the design of a multiparametric system are investigated, including the influence of the antibody position and gold nanoparticle size on the results. The detection limits of the method are found to be 10 ng/mL for FABP and 1 ng/mL for TnI, and the coefficient of variation did not exceed 10%. The developed test system allows us to confirm the diagnosis of acute myocardial infraction (N = 9). The analysis time is 15 min.
Об авторах
I. Andreeva
Department of Chemistry, Moscow State University
Email: aposipov@mail.ru
Россия, Moscow
E. Yakovleva
Department of Chemistry, Moscow State University
Email: aposipov@mail.ru
Россия, Moscow
V. Grigorenko
Department of Chemistry, Moscow State University
Email: aposipov@mail.ru
Россия, Moscow
A. Osipov
Department of Chemistry, Moscow State University; National Research Technological University MISiS
Автор, ответственный за переписку.
Email: aposipov@mail.ru
Россия, Moscow; Moscow
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