Quantification of Hexamethylenediamide Bis( N -Monosuccinyl-L-Glutamyl-L-Lysine) in Blood Plasma by HPLC–MS

A. A. Litvin; G. K. Kolyvanov; E. V. Blynskaya; O. G. Grybakina; P. O. Bochkov; R. V. Shevchenko; V. P. Zherdev

doi:10.3103/S0027131419030088

Quantification of Hexamethylenediamide Bis(N-Monosuccinyl-L-Glutamyl-L-Lysine) in Blood Plasma by HPLC–MS

Authors: Litvin A.A.¹, Kolyvanov G.K.¹, Blynskaya E.V.¹, Grybakina O.G.¹, Bochkov P.O.¹, Shevchenko R.V.¹, Zherdev V.P.¹
Affiliations:
1. Zakusov Research Institute of Pharmacology
Issue: Vol 74, No 3 (2019)
Pages: 146-148
Section: Article
URL: https://bakhtiniada.ru/0027-1314/article/view/163936
DOI: https://doi.org/10.3103/S0027131419030088
ID: 163936

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Abstract

The quantitative determination of a new compound hexamethylenediamide bis(N-monosuccinyl-L-glutamyl-L-lysine) (GK-2) in rat blood plasma is developed and validated by the HPLC–MS technique. The method is linear in the range of 10 to 1000 ng/mL. It is shown that 81.62% of GK-2 is recovered from the plasma. The lower limit of its quantification was 10 ng/mL.