Strip-Dried Biofluids for the Detection of Specific Antibodies in Small, Infected Ruminants
- Authors: Saushkin N.Y.1, Samsonova J.V.1,2, Osipov A.P.1,2, Kondakov S.E.1,2, Lysova E.S.3, Elizarova I.A.4, Khaertynov K.S.5, Shuralev E.A.3,4,5
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Affiliations:
- Department of Chemistry
- National University of Science and Technology MISIS
- Department of Applied Ecology, Institute of Environmental Sciences
- Laboratory of Biochemistry and Molecular-Genetic Analysis, Federal Center of Toxicological
- Central Research Laboratory, Kazan State Medical Academy, Branch of the Federal State Budgetary Educational Institution of Further Professional Education
- Issue: Vol 73, No 3 (2018)
- Pages: 135-137
- Section: Article
- URL: https://bakhtiniada.ru/0027-1314/article/view/163679
- DOI: https://doi.org/10.3103/S0027131418030069
- ID: 163679
Cite item
Abstract
The specific antibodies for viral arthritis-encephalitis and toxoplasmosis in goats is comparatively determined by the ELISA and latex agglutination reaction using strip-dried samples of serum and whole blood on a porous membrane carrier. It is shown that the use of strip-dried samples makes it possible to qualitatively and quantitatively determine specific antibodies and its results are completely consistent with those of the analysis of the liquid samples (serum). This sample preparation method can be used for the safe shipment of blood samples and following serological studies in epizootic monitoring.
About the authors
N. Yu. Saushkin
Department of Chemistry
Author for correspondence.
Email: sushk_90@mail.ru
Russian Federation, Moscow, 119991
J. V. Samsonova
Department of Chemistry; National University of Science and Technology MISIS
Email: sushk_90@mail.ru
Russian Federation, Moscow, 119991; Moscow, 119991
A. P. Osipov
Department of Chemistry; National University of Science and Technology MISIS
Email: sushk_90@mail.ru
Russian Federation, Moscow, 119991; Moscow, 119991
S. E. Kondakov
Department of Chemistry; National University of Science and Technology MISIS
Email: sushk_90@mail.ru
Russian Federation, Moscow, 119991; Moscow, 119991
E. S. Lysova
Department of Applied Ecology, Institute of Environmental Sciences
Email: sushk_90@mail.ru
Russian Federation, Kazan, Tatarstan, 420008
I. A. Elizarova
Laboratory of Biochemistry and Molecular-Genetic Analysis, Federal Center of Toxicological
Email: sushk_90@mail.ru
Russian Federation, Kazan, Tatarstan, 420075
K. S. Khaertynov
Central Research Laboratory, Kazan State Medical Academy, Branch of the Federal State Budgetary Educational Institution of Further Professional Education
Email: sushk_90@mail.ru
Russian Federation, Kazan, Tatarstan, 420012
E. A. Shuralev
Department of Applied Ecology, Institute of Environmental Sciences; Laboratory of Biochemistry and Molecular-Genetic Analysis, Federal Center of Toxicological; Central Research Laboratory, Kazan State Medical Academy, Branch of the Federal State Budgetary Educational Institution of Further Professional Education
Email: sushk_90@mail.ru
Russian Federation, Kazan, Tatarstan, 420008; Kazan, Tatarstan, 420075; Kazan, Tatarstan, 420012
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