Strip-Dried Biofluids for the Detection of Specific Antibodies in Small, Infected Ruminants


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Abstract

The specific antibodies for viral arthritis-encephalitis and toxoplasmosis in goats is comparatively determined by the ELISA and latex agglutination reaction using strip-dried samples of serum and whole blood on a porous membrane carrier. It is shown that the use of strip-dried samples makes it possible to qualitatively and quantitatively determine specific antibodies and its results are completely consistent with those of the analysis of the liquid samples (serum). This sample preparation method can be used for the safe shipment of blood samples and following serological studies in epizootic monitoring.

About the authors

N. Yu. Saushkin

Department of Chemistry

Author for correspondence.
Email: sushk_90@mail.ru
Russian Federation, Moscow, 119991

J. V. Samsonova

Department of Chemistry; National University of Science and Technology MISIS

Email: sushk_90@mail.ru
Russian Federation, Moscow, 119991; Moscow, 119991

A. P. Osipov

Department of Chemistry; National University of Science and Technology MISIS

Email: sushk_90@mail.ru
Russian Federation, Moscow, 119991; Moscow, 119991

S. E. Kondakov

Department of Chemistry; National University of Science and Technology MISIS

Email: sushk_90@mail.ru
Russian Federation, Moscow, 119991; Moscow, 119991

E. S. Lysova

Department of Applied Ecology, Institute of Environmental Sciences

Email: sushk_90@mail.ru
Russian Federation, Kazan, Tatarstan, 420008

I. A. Elizarova

Laboratory of Biochemistry and Molecular-Genetic Analysis, Federal Center of Toxicological

Email: sushk_90@mail.ru
Russian Federation, Kazan, Tatarstan, 420075

K. S. Khaertynov

Central Research Laboratory, Kazan State Medical Academy, Branch of the Federal State Budgetary Educational Institution of Further Professional Education

Email: sushk_90@mail.ru
Russian Federation, Kazan, Tatarstan, 420012

E. A. Shuralev

Department of Applied Ecology, Institute of Environmental Sciences; Laboratory of Biochemistry and Molecular-Genetic Analysis, Federal Center of Toxicological; Central Research Laboratory, Kazan State Medical Academy, Branch of the Federal State Budgetary Educational Institution of Further Professional Education

Email: sushk_90@mail.ru
Russian Federation, Kazan, Tatarstan, 420008; Kazan, Tatarstan, 420075; Kazan, Tatarstan, 420012

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