Characterization and stress-protective action of Saccharomyces cerevisiae extracellular peptide factors on propionic acid bacteria
- 作者: Vorob’eva L.I.1, Rogozhin E.A.2,3, Khodzhaev E.Y.1, Volodyashkin R.A.1, Samoilenko V.A.4
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隶属关系:
- Faculty of Biology
- Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
- Gause Institute of New Antibiotics
- Skryabin Institute of Biochemistry and Physiology of Microorganisms
- 期: 卷 86, 编号 6 (2017)
- 页面: 698-707
- 栏目: Experimental Articles
- URL: https://bakhtiniada.ru/0026-2617/article/view/163280
- DOI: https://doi.org/10.1134/S0026261717060157
- ID: 163280
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详细
Protective effect of Saccharomyces cerevisiae exometabolites (RF1 and RF2) on seven strains of propionic acid bacteria under exposure to bile salts and acid stress was studied. RF2 had a stronger protective effect than RF1. Comparison of the analytical profiles of RF1 and RF2, separated by RP-HPLC, showed that nearly 80% of RF1 components were present in RF2. Separation of S. cerevisiae RF1 into individual components by means of semi-preparative RP-HPLC was used for structural characterization of the active component of the RF1 fraction. It was found to contain two pentapeptides (<1 kDa) with identical amino acid sequences (1Gly-Pro-Tre-Gly-Pro5), differing in one hexose residue. The RF2 separation method based on three-stage liquid chromatography at low and high pressure was developed, which involved analytical high-pressure gel chromatography. The use of the latter method made it possible to separate high-molecular components and perform enrichment of low-molecular peptides. Peptide origin of the RF2 active substances with molecular masses allegedly below 300 Da was confirmed.
作者简介
L. Vorob’eva
Faculty of Biology
编辑信件的主要联系方式.
Email: livorobjeva@mail.ru
俄罗斯联邦, Moscow
E. Rogozhin
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry; Gause Institute of New Antibiotics
Email: livorobjeva@mail.ru
俄罗斯联邦, Moscow; Moscow
E. Khodzhaev
Faculty of Biology
Email: livorobjeva@mail.ru
俄罗斯联邦, Moscow
R. Volodyashkin
Faculty of Biology
Email: livorobjeva@mail.ru
俄罗斯联邦, Moscow
V. Samoilenko
Skryabin Institute of Biochemistry and Physiology of Microorganisms
Email: livorobjeva@mail.ru
俄罗斯联邦, Pushchino
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