A Novel Phosphate-Accumulating Bacterium Identified in a Bioreactor for Phosphate Removal from Wastewater


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Abstract—Biotechnologies involving phosphate-accumulating organisms, which collect inorganic phosphates from the medium as polyphosphates during cyclic growth under aerobic and anaerobic conditions, are presently applied for phosphorus removal from wastewater. Betaproteobacteria of the candidate genus ‘Candidatus Accumulibacter’ carry out phosphate accumulation in most systems for wastewater treatment. However, no member of ‘Ca. Accumulibacter’ has been isolated in pure culture. Metagenomic analysis of the microbial consortium formed in a laboratory setup for phosphate removal from wastewater and removing up to 80% phosphorus from the medium was carried out. Members of the phyla Proteobacteria (82.5%), Bacteroidetes (10.5%), and Chloroflexi (1.6%) predominated in the community. Among the proteobacteria, Betaproteobacteria were revealed, among which ‘Ca. Accumulibacter’ predominated, as well as Gammaproteobacteria (26.8%), which mainly belonged to the family ‘Ca. Competibacteraceae.’ Metagenomic data were used to obtain the genome of the dominant phosphate-accumulating bacterium, which belonged to a new ‘Ca. Accumulibacter’ species. The studied community was promising both for further basic research on metabolism of phosphate-accumulating organisms and for improvement of existing biotechnologies for phosphorus removal from wastewater.

Sobre autores

R. Kotlyarov

Institute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences

Email: mardanov@biengi.ac.ru
Rússia, Moscow, 119071

A. Beletsky

Institute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences

Email: mardanov@biengi.ac.ru
Rússia, Moscow, 119071

A. Kallistova

Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences

Email: mardanov@biengi.ac.ru
Rússia, Moscow, 119071

A. Dorofeev

Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences

Email: mardanov@biengi.ac.ru
Rússia, Moscow, 119071

Yu. Nikolaev

Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences

Email: mardanov@biengi.ac.ru
Rússia, Moscow, 119071

N. Pimenov

Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences

Email: mardanov@biengi.ac.ru
Rússia, Moscow, 119071

N. Ravin

Institute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences

Email: mardanov@biengi.ac.ru
Rússia, Moscow, 119071

A. Mardanov

Institute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences

Autor responsável pela correspondência
Email: mardanov@biengi.ac.ru
Rússia, Moscow, 119071

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