Microbial community associated with Thioploca sp. sheaths in the area of the posolsk bank methane seep, southern baikal


Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
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Рұқсат жабық Тек жазылушылар үшін

Аннотация

Bacterial mats formed by a colorless sulfur bacterium Thioploca sp. in the area of the Posolsk Bank cold methane seep (southern Baikal) were studied using electron microscopy and phylogenetic analysis. Morphologically the bacteria were identified as Thioploca ingrica. Confocal microscopy of DAPI-stained samples revealed numerous rod-shaped, filamentous, and spiral microorganisms in the sheaths, as well as inside and between the trichomes. Transmission electron microscopy revealed nonvacuolated bacteria and small cells without cell envelopes within the sheath. Bacteria with pronounced intracytoplasmic membranes characteristic of type I methanotrophs were observed at the outer side of the sheath. Based on analysis of the 16S rRNA gene sequences, the following phyla were identified in the sheath community: Bacteroidetes, Nitrospira, Chloroflexi, Planctomycetes, Verrucomicrobia, γ-, and δ-Proteobacteria, Euryarchaeota, Crenarchaeota, and Thaumarchaeota, as well as anammox bacteria. A hypothetical scheme of matter flows in the Lake Baikal bacterial mats was proposed based on the data on metabolism of the cultured homologues.

Авторлар туралы

S. Chernitsyna

Limnological Institute, Siberian Branch

Хат алмасуға жауапты Автор.
Email: sveta@lin.irk.ru
Ресей, Irkutsk

I. Khal’zov

Limnological Institute, Siberian Branch

Email: sveta@lin.irk.ru
Ресей, Irkutsk

T. Khanaeva

Limnological Institute, Siberian Branch

Email: sveta@lin.irk.ru
Ресей, Irkutsk

I. Morozov

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch; Novosibirsk State University

Email: sveta@lin.irk.ru
Ресей, Novosibirsk; Novosibirsk

I. Klimenkov

Limnological Institute, Siberian Branch

Email: sveta@lin.irk.ru
Ресей, Irkutsk

N. Pimenov

Winogradsky Institute of Microbiology

Email: sveta@lin.irk.ru
Ресей, Moscow

T. Zemskaya

Limnological Institute, Siberian Branch

Email: sveta@lin.irk.ru
Ресей, Irkutsk

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© Pleiades Publishing, Ltd., 2016