Microbial community associated with Thioploca sp. sheaths in the area of the posolsk bank methane seep, southern baikal
- Авторлар: Chernitsyna S.M.1, Khal’zov I.A.1, Khanaeva T.A.1, Morozov I.V.2,3, Klimenkov I.V.1, Pimenov N.V.4, Zemskaya T.I.1
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Мекемелер:
- Limnological Institute, Siberian Branch
- Institute of Chemical Biology and Fundamental Medicine, Siberian Branch
- Novosibirsk State University
- Winogradsky Institute of Microbiology
- Шығарылым: Том 85, № 5 (2016)
- Беттер: 562-569
- Бөлім: Experimental Articles
- URL: https://bakhtiniada.ru/0026-2617/article/view/162791
- DOI: https://doi.org/10.1134/S0026261716050027
- ID: 162791
Дәйексөз келтіру
Аннотация
Bacterial mats formed by a colorless sulfur bacterium Thioploca sp. in the area of the Posolsk Bank cold methane seep (southern Baikal) were studied using electron microscopy and phylogenetic analysis. Morphologically the bacteria were identified as Thioploca ingrica. Confocal microscopy of DAPI-stained samples revealed numerous rod-shaped, filamentous, and spiral microorganisms in the sheaths, as well as inside and between the trichomes. Transmission electron microscopy revealed nonvacuolated bacteria and small cells without cell envelopes within the sheath. Bacteria with pronounced intracytoplasmic membranes characteristic of type I methanotrophs were observed at the outer side of the sheath. Based on analysis of the 16S rRNA gene sequences, the following phyla were identified in the sheath community: Bacteroidetes, Nitrospira, Chloroflexi, Planctomycetes, Verrucomicrobia, γ-, and δ-Proteobacteria, Euryarchaeota, Crenarchaeota, and Thaumarchaeota, as well as anammox bacteria. A hypothetical scheme of matter flows in the Lake Baikal bacterial mats was proposed based on the data on metabolism of the cultured homologues.
Негізгі сөздер
Авторлар туралы
S. Chernitsyna
Limnological Institute, Siberian Branch
Хат алмасуға жауапты Автор.
Email: sveta@lin.irk.ru
Ресей, Irkutsk
I. Khal’zov
Limnological Institute, Siberian Branch
Email: sveta@lin.irk.ru
Ресей, Irkutsk
T. Khanaeva
Limnological Institute, Siberian Branch
Email: sveta@lin.irk.ru
Ресей, Irkutsk
I. Morozov
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch; Novosibirsk State University
Email: sveta@lin.irk.ru
Ресей, Novosibirsk; Novosibirsk
I. Klimenkov
Limnological Institute, Siberian Branch
Email: sveta@lin.irk.ru
Ресей, Irkutsk
N. Pimenov
Winogradsky Institute of Microbiology
Email: sveta@lin.irk.ru
Ресей, Moscow
T. Zemskaya
Limnological Institute, Siberian Branch
Email: sveta@lin.irk.ru
Ресей, Irkutsk
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