Enviar artigo por via de e-mail Degradation of albumin and aromatic amino acids in reactions with radicals OH● and under the influence of UV radiation 253.7 nm
- Autores: Piskarev I.M.1
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Afiliações:
- D.V. Skobeltsyn Research Institute of Nuclear Physics, Lomonosov Moscow State University
- Edição: Volume 59, Nº 5 (2025)
- Páginas: 344-350
- Seção: RADIATION CHEMISTRY
- URL: https://bakhtiniada.ru/0023-1193/article/view/307644
- DOI: https://doi.org/10.31857/S0023119325050069
- EDN: https://elibrary.ru/bkxsdd
- ID: 307644
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Resumo
Oxidative degradation of bovine serum albumin, a mixture of fluorescent amino acids tyrosine, tryptophan and phenylalanine and separately the same aromatic amino acids included in albumin, under the action of hydroxyl and hydroperoxyl radicals and UV radiationl = 253.7 nm was studied. The starting substances and reaction products were identified by fluorescence method in two-dimensional scanning mode. Stern-Folmer fluorescence quenching coefficients were calculated to analyze the results. Based on the Stern-Folmer coefficients, the reaction mechanisms of radicals and UV radiation with protein and individual amino acids are compared.
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Sobre autores
I. Piskarev
D.V. Skobeltsyn Research Institute of Nuclear Physics, Lomonosov Moscow State University
Email: i.m.piskarev@gmail.com
Moscow, 119234, Russia
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