Optimization of the Method for Measuring 2,3-Diphosphoglycerate in Erythrocytes Using 31P NMR Analysis

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Abstract

It has been shown by 31P NMR spectroscopy that reducing the number of washes of rat erythrocytes significantly increases the stability of 2,3-diphosphoglycerate in these samples. The level of 2,3-diphosphoglycerate remains unchanged for 3.5 hours with a single wash and for 4 hours without washing. At the same time, with standard double washing, the content of 2,3-diphosphoglycerate decreases already after 2.5 hours. Analysis of its distribution between red blood cells and removed plasma, as well as analysis of the serum of one animal, showed that all 2,3-diphosphoglycerate remains in the suspension of red blood cells and is absent in plasma and serum. The results obtained should be taken into account when working with intact erythrocytes.

About the authors

M. V Molchanov

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences

Pushchino, Moscow Region, Russia

A. Ye Yegorov

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences

Pushchino, Moscow Region, Russia

A. V Kholina

Branch of Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Pushchino, Moscow Region, Russia

E. A Kalabina

Branch of Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Pushchino, Moscow Region, Russia

N. A Borozdina

Branch of Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Pushchino, Moscow Region, Russia

S. I Baydarova

Branch of Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Pushchino, Moscow Region, Russia

M. S Kazakova

Branch of Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Pushchino, Moscow Region, Russia

E. V Arshintseva

LLC “Medical emulsions”

Serpukhov, Moscow Region, Russia

S. Yu Pushkin

LLC “Medical emulsions”

Serpukhov, Moscow Region, Russia

M. A Timchenko

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences

Email: maria_timchenko@mail.ru
Pushchino, Moscow Region, Russia

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