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Post a Comment Participation of HIF-1 in the mechanisms of neuroadaptation to acute stressful exposure
- Authors: Lyubimov A.V.1, Khokhlov P.P.1
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Affiliations:
- Institute of Experimental Medicine
- Issue: Vol 19, No 2 (2021)
- Pages: 183-188
- Section: Original study articles
- URL: https://bakhtiniada.ru/RCF/article/view/77305
- DOI: https://doi.org/10.17816/RCF192183-188
- ID: 77305
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Abstract
BACKGROUND: New laboratory and instrumental technologies for analyzing the adaptive capabilities of a biological organism to acute stressful effects including hypoxic ones have significantly facilitated the diagnosis and fixation of adaptive behavioral reactions, physiological and biochemical changes. Much attention has been paid to the phenomenon of preconditioning — a positive effect from exposure to small doses of pathogenic factors. Hypoxia-inducible factor 1 (HIF-1) is one of the most promising markers for fixing the phenomenon of hypoxic preconditioning.
AIM: To study the mechanisms of neuroadaptation to acute stressful effects.
MATERIALS AND METHODS: Using models of immobilization, hypothermic exposure, and electrocutaneous irritation of rat paws were carried out for the assessment of the mechanisms of neuroadaptation. Changes in the HIF-1 concentration were recorded in the blood and in the structures of the brain.
RESULTS: The maximum concentration of HIF-1 was found in the amygdala (±230 pg / mg), in the prefrontal cortex it was ±50.8 pg / mg in the control group. Hypothermal exposure increased the HIF-1 content in the amygdala by more than 4 times, while emotionally pain and immobilization showed a slight decrease in HIF-1 in the amygdala. All types of stressors significantly increased the concentration of HIF-1 in the prefrontal cortex of animals. The most pronounced changes were observed when using the model of emotional pain stress. The obtained experimental data allow us to draw with caution a conclusion about the universality and unity of multicomponent mechanisms of adaptation to acute stressful effects.
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##article.viewOnOriginalSite##About the authors
Andrey V. Lyubimov
Institute of Experimental Medicine
Author for correspondence.
Email: lyubimov_av@mail.ru
ORCID iD: 0000-0001-9829-4681
SPIN-code: 5307-4186
PhD (Medicine)
Russian Federation, 12, Acad. Pavlov str., Saint Petersburg, 197376Platon P. Khokhlov
Institute of Experimental Medicine
Email: platonkh@list.ru
ORCID iD: 0000-0001-6553-9267
SPIN-code: 8673-7417
PhD (Biochemistry)
Russian Federation, 12, Acad. Pavlov str., Saint Petersburg, 197376References
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