Email this article Analysis of the efficiency of obtaining transmitochondrial mice by microinjection of human mitochondria into mouse zygote
- Authors: Bass М.G.1, Sokolova V.A.1, Kustova М.Е.1, Kidgotko О.V.1, Sorokin А.V.1, Vasilyev V.В.1
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Affiliations:
- Institute for Experimental Medicine of the Russian Academy of Medical Sciences
- Issue: Vol 5, No 1 (2005)
- Pages: 55-61
- Section: Basis medicine
- URL: https://bakhtiniada.ru/MAJ/article/view/356134
- ID: 356134
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Abstract
We have elaborated the conditions for transfer of human mitochondria into murine zygotes with subsequent detection of human mt-DNA at various stages of embryonic development and in organs of neonate mice. This allowed obtaining heteroplasmic transmitochondrial animals with human mt-DNA. Such animals can be a useful model for studying maternally inherited mitochondrial diseases. Statistical analysis of the efficiency of obtaining transmitochondrial mice demonstrates high probability of achieving a positive result in a study involving relatively small groups of laboratory animals.
About the authors
М. G. Bass
Institute for Experimental Medicine of the Russian Academy of Medical Sciences
Author for correspondence.
Email: shabanov@mail.rcom.ru
Russian Federation, St. Petersburg
V. A. Sokolova
Institute for Experimental Medicine of the Russian Academy of Medical Sciences
Email: shabanov@mail.rcom.ru
Russian Federation, St. Petersburg
М. Е. Kustova
Institute for Experimental Medicine of the Russian Academy of Medical Sciences
Email: shabanov@mail.rcom.ru
Russian Federation, St. Petersburg
О. V. Kidgotko
Institute for Experimental Medicine of the Russian Academy of Medical Sciences
Email: shabanov@mail.rcom.ru
Russian Federation, St. Petersburg
А. V. Sorokin
Institute for Experimental Medicine of the Russian Academy of Medical Sciences
Email: shabanov@mail.rcom.ru
Russian Federation, St. Petersburg
V. В. Vasilyev
Institute for Experimental Medicine of the Russian Academy of Medical Sciences
Email: shabanov@mail.rcom.ru
Russian Federation, St. Petersburg
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