Hemostatic activity in vitro of lectin-containing substances of bryathypes on the example of some Antarctic and Belarus representatives

Cover Page

Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription Access

Abstract

BACKGROUND: The modern market of medical devices in Belarus and Russia is represented by a wide range of hemostatic agents, of which the most popular are local hemostatics of plant origin possessing the significant technological potential for renewal and improvement. A promising reserve for this may be biologically active compounds of mosses, which are characterized by anti-inflammatory, antibacterial and antifungal effects. However, their hemostatic effect is almost not studied, which determines the relevance of this work.

AIM: The aim of this work is to study the effect of lectin-containing substances from mosses of three species collected in East Antarctica and Belarus on the parameters of human blood hemostasis in vitro.

MATERIALS AND METHODS: We studied mosses of the genera Bryum, Ceratodon, and Coscinodon, collected in the area of the Belarusian Antarctic station Gora Vechernyaya in East Antarctica and in Belarus. Lectin-containing substances of mosses were obtained by extracting shoots in 0.05 M tris-HCl buffer (pH 8.0), centrifugation, filtration. The assessment of the biological activity of lectin-containing substances in mosses was carried out by the agglutination reaction of rabbit erythrocytes, as well as the effect on human platelet aggregation and in the test for activated partial thromboplastin time.

RESULTS: It was established that lectin-containing substances of the studied moss species had agglutinating activity against erythrocytes in the range from 11708.28 (Belarusian samples) to 1333979.59 U/mg of protein (Antarctic samples) depending on the species and localization; initiated the aggregation of human platelets (25–80% of the effect of thrombin) regardless of blood group, Rh and gender of donors; influenced the plasma link of hemostasis, reducing activated partial thromboplastin time (by 15–18%).

CONCLUSIONS: It was found that some species of mosses of the genera Bryum, Ceratodon and Coscinodon of Antarctica and Belarus had an agglutinating and hemostatic effect on erythrocytes and platelets, with the greatest activity noted for Antarctic species. A hypothesis has been put forward that the observed phenomenon is due to the structural features of proteins, including lectins. It is assumed that lectins are possible inducers of erythrocyte agglutination and platelet aggregation in mosses. It is shown that the moss species Bryum pseudotriquetrum and Ceratodon purpureus have a certain resource potential in Belarus for their annual harvest. The results obtained expand the list of moss species with hemostatic activity, and can be used to develop new hemostatics of plant origin for local use from Belarusian plant materials.

About the authors

Olga L. Kandelinskaya

Institute of Experimental Botany National Academy of Sciences of Belarus

Email: okandy@yandex.ru
ORCID iD: 0000-0002-0364-9548
SPIN-code: 2189-4733

Cand. Sci. (Biol.), Assistant Professor, Leading Researcher, Sector of Plant Protein Metabolism and Functions

Belarus, Minsk

Helena R. Grischenko

Institute of Experimental Botany National Academy of Sciences of Belarus

Email: helegreen@yandex.ru
ORCID iD: 0000-0002-2163-6759

Senior Research Associate of Sector of Plant Protein Metabolism and Functions

Belarus, Minsk

Daria V. Grigorieva

Belarusian State University

Email: dargr@tut.by
ORCID iD: 0000-0003-0210-5474
SPIN-code: 2479-7785

Cand. Sci. (Biol.), Assistant Professor of the Department of Biophysics, Physics Faculty

Belarus, Minsk

Irina V. Gorudko

Belarusian State University

Email: irinagorudko@gmail.com
ORCID iD: 0000-0002-4737-470X
SPIN-code: 8968-3125

Cand. Sci. (Biol.), Assistant Professor, Assistant Professor of the Department of Biophysics, Physics Faculty

Belarus, Minsk

Dmitriy V. Goreckiy

Institute of Experimental Botany National Academy of Sciences of Belarus

Email: goreckiydmitriy@yandex.by
ORCID iD: 0009-0000-8279-5094

Junior Research Associate of Sector of Plant Protein Metabolism and Functions

Belarus, Minsk

Eleonora V. Dashkevich

Republican Scientific and Practical Center for Transfusiology and Medical Biotechnologies of the Ministry of Health of the Republic of Belarus

Email: eleonoravdoc@gmail.com
ORCID iD: 0009-0000-9711-9371
SPIN-code: 1804-4804

Head of the Laboratory of Transfusiology

Belarus, Minsk

Janna V. Peshnyak

Republican Scientific and Practical Center for Transfusiology and Medical Biotechnologies of the Ministry of Health of the Republic of Belarus

Email: peshnyak@gmail.com
ORCID iD: 0009-0001-3709-3947
SPIN-code: 2496-2585

Leading Research Associate of the Laboratory of Transfusiology

Belarus, Minsk

Natallia A. Bukhvald

Republican Scientific and Practical Center for Transfusiology and Medical Biotechnologies of the Ministry of Health of the Republic of Belarus

Email: morskayaz300@mail.ru
ORCID iD: 0009-0003-4795-3271
SPIN-code: 8599-4504

Research Associate at the Laboratory of Transfusiology

Belarus, Minsk

Oleg M. Maslovsky

Institute of Experimental Botany National Academy of Sciences of Belarus

Email: oleg.maslovsky@tut.by
ORCID iD: 0009-0003-4976-5215
SPIN-code: 7756-0934

Cand. Sci. (Biol.), Head of Sector of Plant Cadastre

Belarus, Minsk

Irina P. Sysoi

Institute of Experimental Botany National Academy of Sciences of Belarus

Email: mastibrotskaya@gmail.com
ORCID iD: 0009-0001-7777-9433
SPIN-code: 1719-9379

Cand. Sci. (Biol.), Senior Research Associate of Sector of Plant Cadastre

Belarus, Minsk

Yury G. Hihiniak

Scientific and Practical Center for Bioresources National Academy of Sciences of Belarus

Email: antarctida_2010@mail.ru
ORCID iD: 0000-0001-8376-3991

Cand. Sci. (Biol.), Assistant Professor, Leading Research Associate of the Sector for Monitoring and Cadastre of the Wildlife

Belarus, Minsk

Egor V. Korzun

Scientific and Practical Center for Bioresources National Academy of Sciences of Belarus

Email: natrix109@gmail.com
ORCID iD: 0009-0002-9318-9625
SPIN-code: 9108-1529

Senior Research Associate of Sector of monitoring and wildlife cadastre

Belarus, Minsk

Valeria A. Kostevich

Institute of Experimental Medicine; Academician Yu.M. Lopukhin Federal Scientific and Clinical Center for Physical and Chemical Medicine of the Federal Biomedical Agency

Email: hfa-2005@yandex.ru
ORCID iD: 0000-0002-1405-1322
SPIN-code: 2726-2921

Cand. Sci. (Biol.), Senior Research Associate of the Department of Molecular Genetics; Research Associate fellow of Department of Biophysics

Russian Federation, Saint Petersburg; Moscow

Mikhail P. Andreev

V.L. Komarov Botanical Institute of the Russian Academy of Sciences

Email: andreev@gmail.com
ORCID iD: 0000-0002-5688-0751
SPIN-code: 6075-6128

Head of the Laboratory of Lichenology and Bryology

Russian Federation, Saint Petersburg

Lyubov E. Kurbatova

V.L. Komarov Botanical Institute of the Russian Academy of Sciences

Author for correspondence.
Email: kurbatovae@binran.ru
ORCID iD: 0000-0003-4695-5331
SPIN-code: 2032-8596

Senior Research Associate of the Laboratory of Lichenology and Bryology

Russian Federation, Saint Petersburg

References

  1. Bondarev GA, Lipatov VA, Lazarenko SV, et al. Analysis of opinion of surgeons on the role of topical hemostatic agents. Pirogov Russian Journal of Surgery. 2020;(8):61–68. (In Russ.) doi: 10.17116/hirurgia202008161
  2. Chernyavsky AM, Grigor’ev IA, Morozov SV, et al. Local hemostasis control by using of oxidized cellulose drugs. Pirogov Russian Journal of Surgery. 2014;(8):71–75. (In Russ.)
  3. Lipatov VA, Severinov DA, Saakyan AR. Local applicational blood reestablishing instruments in surgery of the XXI century. Innova. 2019;(1):16–22. (In Russ.) doi: 10.21626/innova/2019.1/03
  4. Podterob AP, Zubets EV. A history of the medicinal use of plants of the genus Sphagnum. Pharm Chem J. 2002;36(4):192–194. doi: 10.1023/A:1019884605441
  5. Babeshina LG, Kelus NV, Kotlyar M. History and perspectives of Sphagnum mosses in medicine. Vrach. 2016;(12):31–33. (In Russ.)
  6. Asakawa Y. Biologically active compounds from bryophytes. Pure Appl Chem. 2007:79(4):557–580. doi: 10.1351/pac200779040557
  7. Klavina L, Springe G, Nikolajeva V, et al. Chemical composition analysis, antimicrobial activity and cytotoxicity screening of moss extracts (moss phytochemistry). Molecules. 2015;20(9):17221–17243. doi: 10.3390/molecules200917221
  8. Drobnik J, Stebel A. Four centuries of medicinal mosses and liverworts in European ethnopharmacy and scientific pharmacy: a review. Plants (Basel). 2021;10(7):1296. doi: 10.3390/plants10071296
  9. Benek A, Canlı K, Altuner EM. Traditional medicinal uses of mosses. Anatolian Bryol. 2022;8(1):57–65. doi: 10.26672/anatolianbryology.1061190
  10. Waterman MJ, Nugraha AS, Hendra R, et al. Antarctic moss biflavonoids show high antioxidant and ultraviolet-screening activity. J Nat Prod. 2017;80(8):2224–2231. doi: 10.1021/acs.jnatprod.7b00085
  11. Adebiyi AO, Oyedeji AA, Chikwendu EE, Fatoke OA. Phytochemical screening of two tropical moss plants: Thidium gratum P. beauv and Barbula indica brid grown in Southwestern ecological zone of Nigeria. Am J Anal Chem. 2012;(3):836–839. doi: 10.4236/ajac.2012.312110
  12. Elkhateeb WA, Daba GM. Occurrence of terpenes, polyketides, and tannins in some Japanese lichens and green mosses. Egypt Pharm J. 2021;(26):216–223. doi: 10.4103/epj.epj_17_20
  13. Ebrahimi F, Torbati M, Mahmoudi F, Valizadeh H. Medicinal plants as potential hemostatic agents. J Pharm Pharm Sci. 2020;23(1):11–23. doi: 10.18433/jpps30446
  14. Marcińczyk N, Gromotowicz-Popławska A, Tomczyk M, Chabielska E. Tannins as hemostasis modulators. Front Pharmacol. 2022;12:806891. doi: 10.3389/fphar.2021.806891
  15. Liu F, Li L, Tian X, et al. Chemical constituents and pharmacological activities of steroid saponins isolated from Rhizoma Paridis. J Chem. 2021;2021:1–7. doi: 10.1155/2021/1442906
  16. Ma WY, Xie J, Yu LL, et al. Isolation and identification of hemostatic steroidal glycosides from Ypsilandra thibetica. Bioorg Chem. 2023;130:106268. doi: 10.1016/j.bioorg.2022.106268
  17. Van Damme EJM. 35 years in plant lectin research: a journey from basic science to applications in agriculture and medicine. Glycoconj J. 2022;39:83–97. doi: 10.1007/s10719-021-10015-x
  18. De Coninck T, Van Damme EJM. Review: The multiple roles of plant lectins. Plant Sci. 2021;313:111096. doi: 10.1016/j.plantsci.2021.111096
  19. Gorudko IV, Buko IV, Cherenkevich SN, et al. Lectin-induced aggregates of blood cells from patients with acute coronary syndromes. Arch Med Res. 2008;(39):674–681. doi: 10.1016/j.arcmed.2008.06.002
  20. Shamova EV, Gorudko IV, Drozd ES, et al. Redox regulation of morphology, cell stiffness, and lectin-induced aggregation of human platelets. Eur Biophys. J. 2011;40:195–208. doi: 10.1007/s00249-010-0639-2
  21. Signorello MG, Leoncini G. The molecular mechanisms involved in lectin-induced human platelet aggregation. Biol Chem. 2017;398(12):1335–1346. doi: 10.1515/hsz-2017-0115
  22. Gorudko IV, Loiko EN, Cherenkevich SN, Timoshenko AV. Formation of stable platelet aggregates by lectin from Solanum tuberosum. Biophysics. 2007;52(5):476–480. doi: 10.1134/S0006350907050041
  23. Cannone N, Convey P, Guglielmin M. Diversity trends of bryophytes in continental Antarctica. Polar Biology. 2012;36(2):259–271. doi: 10.1007/s00300-012-1257-5
  24. Deng L, Qi Y, Liu Z, et al. Effect of tannic acid on blood components and functions. Colloids Surf B Biointerfaces. 2019;184:110505. doi: 10.1016/j.colsurfb.2019.110505
  25. Ochyra R, Lewis Smith RI, Bendarek-Ochyra H. Illustrated moss flora of Antarctica. Cambridge: Cambridge University Press; 2008. 685 p.
  26. European Treaty Series (ETS) No 123. European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes. Strasburg, 18.03.1986 [Internet]. Available from: https://rm.coe.int/168007a6a8. Accessed: 02.08.2023.
  27. Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes [Internet]. Available from: https://ruslasa.ru/wp-content/uploads/2017/06/Directive_201063_rus.pdf. Accessed: 02.08.2023.
  28. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;7(72):248–254. doi: 10.1006/abio.1976.9999
  29. Kotova YN, Podoplelova NA, Obydennyy SI, et al. Binding of coagulation factor XIII zymogen to activated platelet subpopulations: Roles of Integrin αIIbβ3 and Fibrinogen. Thromb Haemost. 2019;119(6):906–915. doi: 10.1055/s-0039-1683912
  30. Maslovsky OM, Levkovich AV, Sysoi IP, et al. The state plant cadastre of belarus. fundamentals of the cadastre. Primary survey 2002–2017 years. Minsk: Belorusskaya nauka; 2019. (In Russ.)

Supplementary files

Supplementary Files
Action
1. JATS XML
2. Fig. 1. Moss Bryum pseudotriquetrum: a — distribution map (https://www.gbif.org/species/2676867); b — curtain; с — shoots (photos courtesy of L.E. Kurbatova)

Download (217KB)
3. Fig. 2. Moss Ceratodon purpureus: a — distribution map (https://www.gbif.org/species/5281381); b — network on low rocky outcrops; c — shoots (photographs by L.E. Kurbatova)

Download (240KB)
4. Fig. 3. Moss Coscinodon lawianus: a — distribution map (https://www.gbif.org/species/8124837); b — sods; c — shoots (photographs by L.E. Kurbatova)

Download (234KB)
5. Fig. 4. Typical kinetic curves of platelet aggregation with the addition of Antarctic moss lectin-containing substances (a–c) at various concentrations and thrombin (d). Platelets (2.5 · 10^8 cells/ml) in PBS containing 1 mM CaCl2, 0.5 mM MgCl2. Sample numbers: No. 1 — B. pseudotriquetrum, No. 2 — C. purpureus, No. 3 — C. lawianus. Concentrations used in figure a: 1 — 0.11, 2 — 0.23, 3 — 0.57, 4 — 1.14, 5 — 2.27 µg/ml; b: 1 — 0.18, 2 — 0.45, 3 — 0.9, 4 — 1.8 µg/ml; с: 1 — 0.12, 2 — 0.3, 3 — 0.6, 4 — 1.2 µg/ml; d — 0.5 µg/ml

Download (321KB)
6. Fig. 5. Dependence of the parameters of platelet aggregation (a — degree, b — rate) initiated by the addition of Antarctic moss lectin-containing substances, on the concentration of these compounds. Platelets (2.5 · 10^8 cells/ml) in PBS containing 1 mM CaCl2, 0.5 mM MgCl2. Sample numbers: No. 1 — B. pseudotriquetrum, No. 2 — C. purpureus, No. 3 — C. lawianus

Download (134KB)
7. Fig. 6. Typical kinetic curves of platelet aggregation with the addition of Belarus moss lectin-containing substances at various concentrations. Platelets (2.5 · 10^8 cells/ml) in PBS containing 1 mM CaCl2, 0.5 mM MgCl2. Sample numbers: No. 4 — B. pseudotriquetrum, No. 5 — C. purpureus. Concentrations used in figure a: 1 — 3.4, 2 — 8.5, 3 — 17, 4 — 34 µg/ml; b: 1 — 1.4, 2 — 3.6, 3 — 7.1, 4 — 14.3 µg/ml

Download (162KB)
8. Fig. 7. Dependence of the parameters of platelet aggregation (a — degree, b — rate) initiated by the addition of Belarus moss lectin-containing substances, on the concentration of these compounds. Platelets (2.5 · 10^8 cells/ml) in PBS containing 1 mM CaCl2, 0.5 mM MgCl2. Sample numbers: No. 4 — B. pseudotriquetrum, No. 5 — C. purpureus

Download (128KB)
9. Fig. 8. Distribution of exploitable stocks of B. pseudotriquetrum raw materials on the territory of the Republic of Belarus by administrative regions

Download (199KB)

Copyright (c) 2023 Eco-Vector

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Согласие на обработку персональных данных с помощью сервиса «Яндекс.Метрика»

1. Я (далее – «Пользователь» или «Субъект персональных данных»), осуществляя использование сайта https://journals.rcsi.science/ (далее – «Сайт»), подтверждая свою полную дееспособность даю согласие на обработку персональных данных с использованием средств автоматизации Оператору - федеральному государственному бюджетному учреждению «Российский центр научной информации» (РЦНИ), далее – «Оператор», расположенному по адресу: 119991, г. Москва, Ленинский просп., д.32А, со следующими условиями.

2. Категории обрабатываемых данных: файлы «cookies» (куки-файлы). Файлы «cookie» – это небольшой текстовый файл, который веб-сервер может хранить в браузере Пользователя. Данные файлы веб-сервер загружает на устройство Пользователя при посещении им Сайта. При каждом следующем посещении Пользователем Сайта «cookie» файлы отправляются на Сайт Оператора. Данные файлы позволяют Сайту распознавать устройство Пользователя. Содержимое такого файла может как относиться, так и не относиться к персональным данным, в зависимости от того, содержит ли такой файл персональные данные или содержит обезличенные технические данные.

3. Цель обработки персональных данных: анализ пользовательской активности с помощью сервиса «Яндекс.Метрика».

4. Категории субъектов персональных данных: все Пользователи Сайта, которые дали согласие на обработку файлов «cookie».

5. Способы обработки: сбор, запись, систематизация, накопление, хранение, уточнение (обновление, изменение), извлечение, использование, передача (доступ, предоставление), блокирование, удаление, уничтожение персональных данных.

6. Срок обработки и хранения: до получения от Субъекта персональных данных требования о прекращении обработки/отзыва согласия.

7. Способ отзыва: заявление об отзыве в письменном виде путём его направления на адрес электронной почты Оператора: info@rcsi.science или путем письменного обращения по юридическому адресу: 119991, г. Москва, Ленинский просп., д.32А

8. Субъект персональных данных вправе запретить своему оборудованию прием этих данных или ограничить прием этих данных. При отказе от получения таких данных или при ограничении приема данных некоторые функции Сайта могут работать некорректно. Субъект персональных данных обязуется сам настроить свое оборудование таким способом, чтобы оно обеспечивало адекватный его желаниям режим работы и уровень защиты данных файлов «cookie», Оператор не предоставляет технологических и правовых консультаций на темы подобного характера.

9. Порядок уничтожения персональных данных при достижении цели их обработки или при наступлении иных законных оснований определяется Оператором в соответствии с законодательством Российской Федерации.

10. Я согласен/согласна квалифицировать в качестве своей простой электронной подписи под настоящим Согласием и под Политикой обработки персональных данных выполнение мною следующего действия на сайте: https://journals.rcsi.science/ нажатие мною на интерфейсе с текстом: «Сайт использует сервис «Яндекс.Метрика» (который использует файлы «cookie») на элемент с текстом «Принять и продолжить».