Effect of the S-nitrosoglutathione nitric oxide donor on the relative amount of P-selectin in vitro
- Autores: Korotkova N.V.1, Kalinin R.E.1, Suchkov I.A.1, Abalenikhina Y.V.1, Mzhavanadze N.D.1, Nikiforov A.A.1, Fomina D.I.1
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Afiliações:
- Ryazan State Medical University
- Edição: Volume 23, Nº 3 (2025)
- Páginas: 101-107
- Seção: Original research
- URL: https://bakhtiniada.ru/1728-2918/article/view/312106
- DOI: https://doi.org/10.29296/24999490-2025-03-13
- ID: 312106
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Introduction. Studying the effect of the nitric oxide (II) donor, S-nitrosoglutathione, on the relative amount of selectin P in HUVEC cell lysates in vitro may contribute to fundamental understanding of the effect of the nitric oxide system on the adhesive function of the endothelium.
Objective: To evaluate the effect of nitric oxide (II) donor S-nitrosoglutathione (GSNO) on the activity of mitochondrial endotheliocyte oxidoreductases, the relative amount of selectin P and nitric oxide metabolites in HUVEC cell lysates, and the absolute amount of sP-selectin (soluble) in a conditioned medium.
Material and methods. The mitochondrial dehydrogenase activity of HUVEC cells was evaluated using an MTT test. The relative amount of P-selectin in endotheliocyte lysates was determined by the Western blot method, the absolute amount of the soluble selectin fragment (sP) in the cellular supernatant was determined by the sandwich ELISA method, the level of persistent nitric oxide (II) metabolites was determined by the method based on the reduction of nitrates to nitrites in the presence of vanadium chloride and subsequent spectrophotometric detection using Griss reagent.
Results. The study found that upon joint incubation of the primary HUVEC cell line with nitrosoglutathione solutions in various concentrations, the development of nitrosative stress is noted, as evidenced by an increase in its markers – persistent nitric oxide (II) metabolites in cell lysates. Under conditions of nitrosative stress, there is a decrease in the activity of mitochondrial endotheliocyte oxidoreductases, which may be due to the negative effects of excess nitric oxide or its toxic products. With a three-hour exposure of nitrosoglutathione to endotheliocytes at a low concentration of 10 mkM, there is a statistically significant increase in the relative amount of P-selectin; at high concentrations of 100-500 mkM, there is a statistically significant decrease in the relative amount of P-selectin.
Conclusion. The results obtained in this study indicate the effect of nitric oxide (II) on the functional response of the endothelium by quantifying the cell adhesion molecule P-selectin.
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##article.viewOnOriginalSite##Sobre autores
Natalya Korotkova
Ryazan State Medical University
Autor responsável pela correspondência
Email: fnv8@yandex.ru
ORCID ID: 0000-0001-7974-2450
Código SPIN: 3651-3813
Associate Professor, аsociate Professor of the Department of Biological Chemistry, Senior researcher at the Central Research laboratory
Rússia, Vysokovoltnaya str., 9, Ryazan, 390026Roman Kalinin
Ryazan State Medical University
Email: r.kalinin@rzgmu.ru
ORCID ID: 0000-0002-0817-9573
Código SPIN: 5009-2318
Doctor of Medical Sciences, Professor in cardiovascular surgery, Head of the Department of cardiovascular, endovascular surgery, and diagnostic radiology
Rússia, Vysokovoltnaya str., 9, Ryazan, 390026Igor Suchkov
Ryazan State Medical University
Email: i.suchkov@rzgmu.ru
ORCID ID: 0000-0002-1292-5452
Código SPIN: 6473-8662
Doctor of Medical Sciences, Professor in cardiovascular surgery, Professor of the Department of cardiovascular, endovascular surgery, and diagnostic radiology
Rússia, Vysokovoltnaya str., 9, Ryazan, 390026Yulia Abalenikhina
Ryazan State Medical University
Email: abalenihina88@mail.ru
ORCID ID: 0000-0003-0427-0967
Doctor of Medical Sciences, PhD in biochemical sciences, Professor of the Department of Biological Chemistry, Leading researcher at the Central Research laboratory
Rússia, Vysokovoltnaya str., 9, Ryazan, 390026Nina Mzhavanadze
Ryazan State Medical University
Email: nina_mzhavanadze@mail.ru
ORCID ID: 0000-0001-5437-1112
Código SPIN: 7757-8854
Doctor of Medical Sciences, PhD in cardiovascular surgery, Professor the department of cardiovascular, endovascular surgery and diagnostic radiology, Leading researcher at the central research laboratory
Rússia, Vysokovoltnaya str., 9, Ryazan, 390026Alexander Nikiforov
Ryazan State Medical University
Email: a.nikiforov@rzgmu.ru
ORCID ID: 0000-0001-9742-4528
Código SPIN: 8366-5282
PhD in pharmaceutical sciences, Associate professor of the department of pharmacology, Head of the Central Research laboratory
Rússia, Vysokovoltnaya str., 9, Ryazan, 390026Darya Fomina
Ryazan State Medical University
Email: fdarya18@yandex.ru
ORCID ID: 0009-0008-5532-6303
5th year student at the Faculty of Medicine
Rússia, Vysokovoltnaya str., 9, Ryazan, 390026Bibliografia
- Smith B.A.H., Bertozzi C.R. The clinical impact of glycobiology: targeting selectins, Siglecs and mammalian glycans. Nat. Rev. Drug. Discov. 2021; 20 (3): 217–43. doi: 10.1038/s41573-020-00093-1.
- Laine R.A. A calculation of all possible oligosaccharide isomers both branched and linear yields 1.05 _ 1012 structures for a reducing hexasaccharide: The Isomer Barrier to development of single-method saccharide sequencing or synthesis systems. Glycobiology. 1994; 4: 759–67. doi: 10.1093/glycob/4.6.759.
- Roseman S. Reflections on glycobiology. J. Biol. Chem. 2001; 276: 41527–42. doi: 10.1074/jbc.R100053200.
- Zhang J., Huang S., Zhu Z., Gatt A., Liu J. E-selectin in vascular pathophysiology. Front Immunol. 2024; 19 (15): 1401399. doi: 10.3389/fimmu.2024.1401399.
- Francisco R., Brasil S., Poejo J., Jaeken J., Pascoal C., Videira P.A., Dos Reis Ferreira V. Congenital disorders of glycosylation (CDG): state of the art in 2022. Orphanet J. Rare Dis. 2023; 18: 329. doi: 10.1186/s13023-023-02879-z.
- McEver R.P. Selectins: initiators of leucocyte adhesionand signalling at the vascular wall. Cardiovasc. Res. 2015; 107: 331–9. doi: 10.1093/cvr/cvv154.
- Tvaroška I., Selvaraj C., Koča J. Selectins – The Two Dr. Jekyll and Mr. Hyde Faces of Adhesion Molecules – A Review. Molecules. 2020; 25 (12): 2835. doi: 10.3390/molecules25122835.
- Cao Y., Song W., Chen X. Multivalent sialic acid materials for biomedical applications. Biomater Sci. 2023; 11 (8): 2620–38. doi: 10.1039/d2bm01595a.
- Kneuer C., Ehrhardt C., Radomski M.W., Bakowsky U. Selectins-potential pharmacological targets? Drug Discov Today. 2006; 11 (21–22): 1034–40. doi: 10.1016/j.drudis.2006.09.004.
- Черных И.В., Копаница М.А., Щулькин А.В., Якушева Е.Н., Ершов А.Ю., Мартыненков А.А., Лагода И.В., Волкова А.М. Оценка цитотоксичности гликонаночастиц золота на клетках аденокарциномы ободочной кишки человека. Российский медико-биологический вестник им. академика И.П. Павлова. 2023; 31 (2): 255–64. doi: 10.17816/PAVLOVJ112525 [Chernyh I.V., Kopanica M.A., Shhul'kin A.V., Jakusheva E.N., Ershov A.Ju., Martynenkov A.A., Lagoda I.V., Volkova A.M. Assessment of cytotoxicity of gold glyconanoparticles on human colon adenocarcinoma cells. Rossijskij mediko-biologicheskij vestnik im. akademika I.P. Pavlova. 2023; 31 (2): 255–64. doi: 10.17816/PAVLOVJ112525 (in Russian)].
- Тризно М.Н., Тризно Е.В., Мажитова М.В., Абакаров А.М., Кривоносова Е.И. Микрофлюидные системы для оценки тромбоцитарных агрегатов на фоне сероводорода и ацетилцистеина. Наука молодых (Eruditio Juvenium). 2024; 12 (3): 418–28. doi: 10.23888/HMJ2024123418-428 [Trizno M.N., Trizno E.V., Mazhitova M.V., Abakarov A.M., Krivonosova E.I. Microfluidic systems for evaluation of platelet aggregates on the background of hydrogen sulfide and acetylcysteine. Nauka molodyh (Eruditio Juvenium). 2024; 12 (3): 418–28. doi: 10.23888/HMJ2024123418-428 (in Russian)].
- Kim J., Islam S.M.T., Qiao F., Singh A.K., Khan M., Won J., Singh I. Regulation of B cell functions by S-nitrosoglutathione in the EAE model. Redox Biol. 2021; 45: 102053. doi: 10.1016/j.redox.2021.102053.
- Broniowska A.K., Diers A.R., Hogg N. S-Nitrosoglutathione. Biochimica et Biophysica Acta (BBA). 2013; 1830 (5): 3173–81. doi: 10.1016/j.bbagen.2013.02.004.
- Путинцева О.В., Калаева Е.А., Артюхов В.Г., Гостева Е.В. S-нитрозоглутатион в высоких концентрациях (75:1) ингибирует кислородсвязывающую функцию оксигемоглобина человека. Вестник Воронежского государственного университета. Серия: Химия. Биология. Фармация. 2018; 4: 66–72 [Putinceva O.V., Kalaeva E.A., Artjuhov V.G., Gosteva E.V. S-nitrosoglutathione in high concentrations (75:1) inhibits the oxygen-binding function of human oxyhemoglobin. Vestnik Voronezhskogo gosudarstvennogo universiteta. Serija: Himija. Biologija. Farmacija. 2018; 4: 66–72 (in Russian)].
- Kansas G.S. Selectins and their ligands: current concepts and controversies. Blood. 1996; 88 (9): 32593287. doi: 10.1182/blood.V88.9.3259.bloodjournal8893259
- Silva M., Videira P.A., Sackstein R. E-Selectin Ligands in the Human Mononuclear Phagocyte System: Implications for Infection, Inflammation, and Immunotherapy. Frontiers in Immunology. 2018; 8: 1878. doi: 10.3389/fimmu.2017.01878
- Ramachandran N., Root P., Jiang X.M., Hogg P.J., Mutus B. Mechanism of transfer of NO from extracellular S-nitrosothiols into the cytosol by cell-surface protein disulfide isomerase. Proc Natl Acad Sci USA. 2001; 98 (17): 9539–44. doi: 10.1073/pnas.171180998.
- Zhang Y., Hogg N. The mechanism of transmembrane S-nitrosothiol transport. Proc Natl Acad Sci USA. 2004; 101 (21): 7891–6. doi: 10.1073/pnas.0401167101.
- Wynia-Smith S.L., Smith B.C. Nitrosothiol formation and S-nitrosation signaling through nitric oxide synthases. Nitric Oxide. 2017; 63: 52–60. doi: 10.1016/j.niox.2016.10.001.
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