电邮这篇文章 Comparing methods for detecting mitochondrial DNA damage using real-time polymerase chain reaction and buccal epithelial micronucleus assay to assess genetic homeostasis in humans
- 作者: Kalaev V.N.1, Zuevsky V.P.2, Larina A.V.1, Kalaeva E.A.1, Nechaeva M.S.3, Zuevskaya T.V.4, Maltseva O.Y.5
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隶属关系:
- Voronezh State University
- Khanty-Mansiysk State Medical Academy
- N.N. Burdenko Voronezh State Medical University
- Sanatorium and Resort Complex "Anapskii"
- Voronezh State University of Engineering Technologies
- 期: 卷 27, 编号 3 (2025)
- 页面: 331-340
- 栏目: Original Study Article
- URL: https://bakhtiniada.ru/1682-7392/article/view/319564
- DOI: https://doi.org/10.17816/brmma642849
- EDN: https://elibrary.ru/MQTJRA
- ID: 319564
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详细
BACKGROUND: Various environmental factors that can affect the stability of the genetic material in humans, using a range of cytological and molecular genetic methods, are being studied actively.
AIM: This work aimed to identify and analyze the associations between the buccal epithelial micronucleus assay results and mitochondrial DNA damage detected using real-time polymerase chain reaction (RT-PCR).
METHODS: The study was conducted in Voronezh; 25 and 10 men and women, respectively, aged 20–24 years, were selected as participants. The buccal epithelial micronucleus assay and mitochondrial DNA damage assessment using RT-PCR were applied. In each sample, ≥2000 cells were examined to determine the number of cells with micronuclei, perinuclear vacuoles, notches, and protrusions. DNA repair and cytogenetic damage accumulation indices were calculated. The DNA was extracted using the CTAB buffer. RT-PCR employing the Encyclo polymerase and selected primers amplified two fragments (short and long) within the D-loop region of mitochondrial DNA. The number of DNA lesions was calculated based on the Ct values by applying a specific formula. Statistical analysis of the data was conducted with the Stadia software package.
RESULTS: The frequency of mitochondrial DNA damage and the occurrence of nuclear abnormalities in the buccal epithelial cells of the study groups were determined. A higher degree of variation in the parameters studied was observed in females compared to males. Correlations were established between mitochondrial DNA damage and the frequency of nuclear abnormalities. Similar patterns of change were observed in mitochondrial DNA damage frequencies and nuclear aberrations.
CONCLUSION: The common patterns of variation identified in the cytogenetic and molecular genetic indicators of genomic stability, as well as the correlations between the frequency of nuclear abnormalities in buccal epithelial cells and mitochondrial DNA damage, suggest a shared etiology of molecular and cell genetic lesions. These findings indicate the potential of utilizing these parameters to predict and refine the values of each other.
作者简介
Vladislav Kalaev
Voronezh State University
Email: z-alnair@mail.ru
ORCID iD: 0000-0002-4247-4509
SPIN 代码: 7022-6720
MD, Dr. Sci. (Biology), Professor
俄罗斯联邦, VoronezhVladislav Zuevsky
Khanty-Mansiysk State Medical Academy
Email: z-alnair@mail.ru
ORCID iD: 0000-0002-4662-9205
SPIN 代码: 7800-5955
MD, Dr. Sci. (Medicine), Professor
俄罗斯联邦, Khanty-MansiyskAnna Larina
Voronezh State University
Email: z-alnair@mail.ru
ORCID iD: 0000-0001-5389-9580
SPIN 代码: 2071-1537
assistant
Elena Kalaeva
Voronezh State University
Email: z-alnair@mail.ru
ORCID iD: 0000-0002-3668-0816
SPIN 代码: 4851-5000
MD, Cand. Sci. (Biology), Associate Professor
俄罗斯联邦, VoronezhMarina Nechaeva
N.N. Burdenko Voronezh State Medical University
Email: z-alnair@mail.ru
ORCID iD: 0000-0003-4880-6751
SPIN 代码: 5108-2660
MD, Cand. Sci. (Biology)
俄罗斯联邦, VoronezhTatiana Zuevskaya
Sanatorium and Resort Complex "Anapskii"
编辑信件的主要联系方式.
Email: z-alnair@mail.ru
ORCID iD: 0000-0002-9315-1320
SPIN 代码: 9759-1894
MD, Dr. Sci. (Medicine), Assistant Professor, address
俄罗斯联邦, 8 Utrishskaya St, Sukko Village, Anapa, 353408Oksana Maltseva
Voronezh State University of Engineering Technologies
Email: z-alnair@mail.ru
ORCID iD: 0000-0002-3815-123X
SPIN 代码: 2670-4258
Cand. Sci. (Engineering), Associate Professor
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