Combination of methods for determine the authenticity of herbs Thymus serpyllum and Thymus marshallianus
- Авторлар: Sheremetyeva A.S.1, Fomina Y.A.1, Shestopalova N.B.1, Durnova N.A.2
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Мекемелер:
- Saratov State Medical University named after V.I. Razumovsky
- Pharmacognosy and Botany, Saratov State Medical University named after V.I. Razumovsky
- Шығарылым: Том 26, № 3 (2023)
- Беттер: 21-26
- Бөлім: Pharmaceutical chemistry
- URL: https://bakhtiniada.ru/1560-9596/article/view/143119
- DOI: https://doi.org/10.29296/25877313-2023-03-03
- ID: 143119
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Аннотация
Relevance. Authentication of crushed medicinal plant raw materials (RM) is an actual issue because there are species similar in morphology or there is a possibility of interspecific hybridization. The characteristics of the absorption spectra of extracts and their chromatographic profile are significant indicators of the authenticity of RS.
The aim of the study was a comparative analysis by TLC and spectrophotometry of water-alcohol extracts from the herb Thymus marshallianus Willd. (TM) and Thymus serpyllum L. (TP).
Material and methods. Water-alcohol extracts were obtained from 1.0 g of RS (exact suspension) in two ways (extractant – 70% ethanol, heating in a boiling water bath; extractant – 50% ethanol, 20–22 °C). Absorption spectra were recorded in the range of 200–500 nm. Eluent – ethyl acetate : formic acid anhydrous : water (70:15:15) was used for chromatography. Detection of adsorption zones was carried out after treatment with a 5% alcohol solution of AlCl3 at 365 nm. Rutin, quercetin and cinaroside were used as standard samples.
Results. Microscopic analysis revealed diagnostic signs of the TM and TP leaf – trichomes of various types. The absorption spectra of water-alcohol extracts from TM and TP (obtained with 50% and 70% ethanol) have two pronounced maxima. When AlCl3 is added, a bathochromic shift of the long-wavelength absorption bands is observed. The chromatograms of these extracts revealed a yellow zone at the level of cinaroside, and differences in the number and color of the adsorption zones of TM and TP extracts.
Conclusion. The absorption spectra of water-alcohol extracts have two pronounced maxima for TP of the same intensity, for TM – different. The intensity of differential absorption spectra of water-alcohol extracts of TM is higher, which indicates a higher content of flavonoids in its extracts. Cinaroside was detected in TM and TP extracts by TLC, while 11 adsorption zones are distinguishable on the chromatogram of extracts from TM herb, and 9 from TP.
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##article.viewOnOriginalSite##Авторлар туралы
A. Sheremetyeva
Saratov State Medical University named after V.I. Razumovsky
Хат алмасуға жауапты Автор.
Email: anna-sheremetyewa@yandex.ru
Senior Lecturer, Department of General Biology, Pharmacognosy and Botany
Ресей, SaratovYu. Fomina
Saratov State Medical University named after V.I. Razumovsky
Email: fominaya@mail.ru
Ph.D. (Chem.), Head of the Laboratory for Research and Quality Control of Medicines
Ресей, SaratovN. Shestopalova
Saratov State Medical University named after V.I. Razumovsky
Email: shestopalovanb@yandex.ru
Ph.D. (Chem.), Senior Lecturer, Department of General, Bioorganic and Pharmaceutical Chemistry
Ресей, SaratovN. Durnova
Pharmacognosy and Botany, Saratov State Medical University named after V.I. Razumovsky
Email: ndurnova@mail.ru
Dr.Sc. (Biol.), Associate Professor, Department of General Biology
Ресей, SaratovӘдебиет тізімі
- Zagrebel'nyj I.A., Dzh Ju.S., Boroniev N. S., Dzhamshedov, D.N. O formirovanii syr'evoj bazy introducirovannyh rastenij dlja razvitija otechestvennogo farmacevticheskogo proizvodstva. Vestnik Akademii medicinskih nauk Tadzhikistana. 2017; 4(24): 102–106.
- Kusova R.D. Issledovanie flavonoidov i oksikorichnyh kislot v trave Thymus marschallianus Willd. Ezhemesjachnyj nauchnyj zhurnal. 2014; 2: 142-144.
- Fedorov A.A., Menickyj Ju.L. Flora evropejskoj chasti SSSR. L.: Nauka. 1978. T. 3. 259 s.
- Starchak Ju.A. Farmakognosticheskoe izuchenie rastenij roda tim'jan (Thymus L.) kak perspektivnogo istochnika poluchenija fitopreparatov: Avtorf. diss …. dokt. farmac. Nauk. Kurskij gosudarstvennyj medicinskij universitet. Kursk. 2016. 470 s.
- Popov A.I., Shpan'ko D.N., Cherkasova E.A., Shajdulina T.B. Sovershenstvovanie metodik identifikacii i ustanovlenija podlinnosti rastitel'nogo syr'ja, «podzemnye organy» prjano-aromaticheskih kul'tur. Tehnika i tehnologija pishhevyh proizvodstv. 2009. 3: 75–78.
- Morozov S.V., Tkacheva N.I., Tkachev A.V. Problemy kompleksnogo himicheskogo profilirovanija lekarstvennyh rastenij. Himija rastitel'nogo syr'ja. 2018; 4: 5–28.
- Gosudarstvennaja farmakopeja XIV izdanie. Tom 1, 2, 4. M. 2018.
- Sheremet'eva A.S., Fomina Ju.A., Shestopalova N.B., Durnova N.A. Vlijanie uslovij jekstrakcii na soderzhanie flavonoidov v izvlechenijah iz travy tim'jana Marshalla i tim'jana polzuchego. Farmacija. 2021; 70(7): 41–46.
- Sheremet'eva A.S., Frolkova A.V., Shapoval O.G. i dr. Soderzhanie i antimikrobnaja aktivnost' jefirnyh masel v trave tim'jana Marshalla i tim'jana polzuchego. Voprosy biologicheskoj, medicinskoj i farmacevticheskoj himii. 2021; 24(3): 27–32.
- Sheremet'eva A.S., Durnova N.A., Berezuckij M.A. Soderzhanie jefirnyh masel v trave raznyh vidov roda tim'jan (Thymus L.). Bjulleten' botanicheskogo sada Saratovskogo gosudarstvennogo universiteta. 2017; 15(2): 15–19.
- Sevastre-Berghian A.C., Ielciu I., Mitre A.O. et al. Targeting oxidative stress reduction and inhibition of HDAC1, MECP2, and NF-kB pathways in rats with experimentally induced hyperglycemia by administration of Thymus marshallianus Willd. Extracts. Front. Pharmacol. 2020; 11: 581470. doi: 10.3389/fphar.2020.581470.
- Ielciu, I., Filip, G. A., Oniga, I. et al. Oxidative stress and DNA lesion reduction of a polyphenolic enriched extract of Thymus marschallianus Willd. in endothelial vascular cells exposed to hyperglycemia. Plant. 2021; 10(12): 2810. doi: 10.3390/plants10122810.
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