电邮这篇文章 Expression proteaseinhibitors receptor PAR-2 on neutrophils peripheral blood of patients with atopic dermatitis and their relation with heat shock proteins HSP90
- 作者: Elistratova I.V1, Morozov S.G.2, Zakharova I.A2
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隶属关系:
- Main Military Clinical Hospital of Internal Troops of the Ministry of Internal Affairs of Russia
- Institute of General Pathology and Pathophysiology
- 期: 卷 19, 编号 1 (2016)
- 页面: 53-58
- 栏目: Articles
- URL: https://bakhtiniada.ru/1560-9588/article/view/37138
- DOI: https://doi.org/10.18821/1560-9588-2016-19-1-53-58
- ID: 37138
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Expression ofprotease-activated receptors PAR-2 has been investigated on neutrophils isolated from atopic dermatitis patient peripheral blood and has been analyzed with index SCORAD. The simultaneous expression of both, PAR-2 receptors and heat shock protein 90 (HSP90) was measured on neutrophils at the first week of acute phase of atopic dermatitis. Methods. Peripheral blood neutrophils were isolated on the Percoll gradient. Cells were stained with antibodies to PAR-2 receptor and HSP90 followed by the flow cytometry. Results. The PAR-2 receptors have been shown to be expressed on the plasma membrane of neutrophils obtained from patients with atopic dermatitis. The percentage of PAR-2+ neutrophils was positive correlated with diseases severity. HSP90 was found to be elevated simultaneously with PAR-2 receptors on neutrophils at the acute phase of atopic relapse. Conclusions. Atopic dermatitis patients have the higher level of PAR-2 receptor expression compared with healthy donors. Percentage of PAR-2+ neutrophils is elevated with the increasing of disease severity. Simultaneous elevation of PAR-2 receptors and HSP90 was found at the first week of acute atopic dermatitis.
作者简介
I. Elistratova
Main Military Clinical Hospital of Internal Troops of the Ministry of Internal Affairs of Russia143915, Balashiha, Russia
Sergey Morozov
Institute of General Pathology and Pathophysiology
Email: biopharm@list.ru
Doctor of Medical Sciences, Professor, Corresponding Member of Russian Academy of Sciences, deputy director for scientific work 125315, Moscow, Russia
I. Zakharova
Institute of General Pathology and Pathophysiology125315, Moscow, Russia
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