Interaction between MSCS and blood mononuclear cells during in vitro co-cultivation in the presence of a three-dimensional artificial matrix mimicking regenerating bone tissue
- 作者: Yurova K.A.1, Norkin I.K.1, Khaziakhmatova O.G.1, Malashchenko V.V.1, Melashchenko O.B.1, Ivanov P.A.1, Ligatyuk D.D.1, Khlusov I.A.1, Litvinova L.S.1
-
隶属关系:
- Immanuel Kant Baltic Federal University
- 期: 卷 26, 编号 4 (2023)
- 页面: 443-448
- 栏目: Forum Sochi 2023
- URL: https://bakhtiniada.ru/1028-7221/article/view/253425
- DOI: https://doi.org/10.46235/1028-7221-13547-IBM
- ID: 253425
如何引用文章
全文:
详细
Bone tissue repair and regeneration is a complex process involving many cells and controlled by multiple factors. Immune cells and cytokines play a crucial role in regulating the balance of bone formation and resorption. However, the immunomodulatory mechanism of bone regeneration is still unclear. Nevertheless, the reciprocal regulatory influence of immunocompetent cells and mesenchymal stem cells (MSCs) is well known. MSCs and immunocompetent cells secrete various cytokines, growth factors, and extracellular matrix molecules that play important roles in regulating hematopoiesis, angiogenesis, immune and inflammatory responses. Several studies confirm that different molecules expressed by MSCs may induce lymphocyte proliferation. Therefore, the study of the mutual influence of MSCs and blood mononuclear cells during in vitro co-cultivation, even in the presence of an artificial matrix mimicking regenerating bone tissue, is relevant and expedient. In this experimental series, the studies were performed at the interface between living and non-living substrate phases thus mimicking the “regenerating bone / hematopoietic microenvironment” system. A series of separated in time experiments was performed on a plastic surface (2D culture model) and in the presence of three-dimensional artificial matrices mimicking regenerating bone tissue (3D culture model).
作者简介
Kristina Yurova
Immanuel Kant Baltic Federal University
编辑信件的主要联系方式.
Email: kristina_kofanova@mail.ru
PhD (Medicine), Senior Research Associate, Center for Immunology and Cellular Biotechnologies, Immanuel Kant Baltic Federal University
俄罗斯联邦, KaliningradI. Norkin
Immanuel Kant Baltic Federal University
Email: kristina_kofanova@mail.ru
PhD (Biology), Technician, Center for Immunology and Cellular Biotechnologies, Immanuel Kant Baltic Federal University
KaliningradO. Khaziakhmatova
Immanuel Kant Baltic Federal University
Email: kristina_kofanova@mail.ru
PhD (Biology), Senior Research Associate, Center for Immunology and Cellular Biotechnologies, Immanuel Kant Baltic Federal University
俄罗斯联邦, KaliningradV. Malashchenko
Immanuel Kant Baltic Federal University
Email: kristina_kofanova@mail.ru
Senior Research Associate, Engineer, Center for Immunology and Cellular Biotechnologies, Immanuel Kant Baltic Federal University
俄罗斯联邦, KaliningradO. Melashchenko
Immanuel Kant Baltic Federal University
Email: kristina_kofanova@mail.ru
Research Associate, Center for Immunology and Cellular Biotechnologies, Immanuel Kant Baltic Federal University
俄罗斯联邦, KaliningradP. Ivanov
Immanuel Kant Baltic Federal University
Email: kristina_kofanova@mail.ru
PhD (Medicine), Junior Research Associate, Center for Immunology and Cellular Biotechnologies, Immanuel Kant Baltic Federal University
俄罗斯联邦, KaliningradD. Ligatyuk
Immanuel Kant Baltic Federal University
Email: kristina_kofanova@mail.ru
Engineer, Center for Immunology and Cellular Biotechnologies, Immanuel Kant Baltic Federal University
俄罗斯联邦, KaliningradI. Khlusov
Immanuel Kant Baltic Federal University
Email: kristina_kofanova@mail.ru
PhD, MD (Medicine), Chief Research Associate, Center for Immunology and Cellular Biotechnologies, Immanuel Kant Baltic Federal University
俄罗斯联邦, KaliningradL. Litvinova
Immanuel Kant Baltic Federal University
Email: kristina_kofanova@mail.ru
PhD, MD (Medicine), Director, Center for Immunology and Cellular Biotechnologies, Immanuel Kant Baltic Federal University
俄罗斯联邦, Kaliningrad参考
- Коршунов Д.А., Кондакова И.В. Современные достижения и проблемы в исследовании культур клеток // Успехи современной биологии, 2016. Т. 136, № 4. С. 347-361. [Korshunov D.A., Kondakova I.V. Modern achievements and problems in the study of cell cultures]. Uspekhi sovremennoy biologii = Advances in Modern Biology, 2016, Vol. 136, no. 4, pp. 347-361. (In Russ.)]
- Khlusov I.A., Litvinova L.S., Shupletsova V.V., Khaziakhmatova O.G., Malashchenko V.V., Yurova K.A., Shunkin E.O., Krivosheev V.V., Porokhova E.D., Sizikova A.E., Safiullina L.A., Legostaeva E.V., Komarova E.G., Sharkeev Y.P. Costimulatory effect of blood cells and rough calcium phosphate coating on the angiogenic and osteogenic features of adipose-derived multipotent mesenchymal stromal cells in mixed culture as a model of postimplantation tissue repair. Materials (Basel), 2020, Vol. 13, no. 19, 4398. doi: 10.3390/ma13194398.
- Legostaeva E.V., Kulyashova K.S., Komarova E.G. Physical, chemical and biological properties of micro-arc deposited calcium phosphate coatings on titanium and zirconium-niobium alloy. Materialwissenschaft und Werkstofftechnik, 2013, Vol. 44, no. 2-3, pp. 188-197.
- Schell H., Duda G.N., Peters A. The haematoma and its role in bone healing. J. Exp. Orthop., 2017, Vol. 4, 5. doi: 10.1186/s40634-017-0079-3.
- Steward A.J., Kelly D.J. Mechanical regulation of mesenchymal stem cell differentiation. J. Anat., 2015, Vol. 227, no. 6, pp. 717-731.
- Yang N., Liu Y. The role of the immune microenvironment in bone regeneration. Int. J. Med. Sci., 2021, Vol. 18, no. 16, pp. 3697-3707.
- Zuk P.A., Zhu M., Mizuno H., Huang J., Futrell J.W., Katz A.J., Benhaim P., Lorenz H.P., Hedrick M.H. Multilineage cells from human adipose tissue: implications for cell-based. Tissue Eng., 2001, Vol. 7, no. 2, pp. 211-228.
补充文件
