INFLUENCE OF OXIDATIVE STRESS ON POSTOPERATIVE PERIOD OF DIABETIC CATARACT SURGERY

The purpose of this study is to reveal the state of the body's antioxidant systems using Western-blot enzyme immunoassay in tears for peroxedoxin-6 (PRDX6) proteins and / or fragments of its disintegration as a marker of protection against oxidative stress in patients with age and diabetic cataracts. Patients and methods. 136 patients (136 eyes) with cataract of various genesis were examined. The control group was represented by 25 volunteers who did not have eye diseases (50 eyes). Two groups of patients were examined. The first group included 78 patients with age-related cataracts. The second group included 58 patients with diabetic complicated cataract. The material of the study was fluids of the tear fluid the of the anterior chamber of patients with cataracts. To determine the active peroxedoxin-6 (PRDX6) and / or its degradation fragments, a Western-blot enzyme-linked immunosorbent assay was used. The expression of enzymes of protection against oxidative stress in samples of tear fluid was studied and a comparative evaluation of the level of activity of lactic acid antioxidant enzymes in oxidative stress after cataract surgery was given. Results. In the postoperative period in patients with age-related cataracts, the increase in the level of PRDX6 is determined, which is confirmed by the absence of complications of FEC. With a complicated cataract, the PRDX6 content is 6 times lower than in patients with age-related cataracts. Conclusion. If there is an active peroxiredoxin 6 in the lacrimal fluid and / or fragments of its decay, age-related cataracts are diagnosed, and this corresponds to a high level of protection against oxidative stress. In such patients, the postoperative period proceeds favorably. In the absence of active peroxiredoxin-6 and / or fragments of its decay in the lacrimal fluid, the presence of cataract due to diabetes mellitus is diagnosed. In the study of anterocameral fluid, a marker of development of metabolic disturbances in complicated cataracts and a 45 kDa transporter of hydrophobic compounds of the family of Sec-14p-like proteins was revealed in patients of group 2. Cataracts are considered diabetic, and in the postoperative period, the risk of developing PEER increases. The use of the method makes it possible to increase the accuracy of the choice of cataract treatment tactics.

diabetes, cataract, peroxiredoxin-6, postoperative exudative-inflammatory reaction, диабет, катаракта, пероксиредоксин-6, послеоперационная экссудативно-воспалительная реакция