Identification of IgE-mediated sensitization in children by enzyme immunoassay and immunofluorescence methods
- Authors: Sokolova T.F.1, Shcheglova E.L.1, Sokolov E.Y.2, Emelyanov Y.V.3
-
Affiliations:
- Omsk State Medical University
- Karpovka Scientific and Clinical Center
- Pirogov Russian National Research Medical University
- Issue: Vol 27, No 2 (2024)
- Pages: 231-236
- Section: SHORT COMMUNICATIONS
- URL: https://bakhtiniada.ru/1028-7221/article/view/263681
- DOI: https://doi.org/10.46235/1028-7221-16710-IOI
- ID: 263681
Cite item
Full Text
Abstract
The goal of research was to determine the frequency of detection and the structure of causally significant sensitization to the main groups of allergens in children with allergic diseases in Siberia when evaluating the results by enzyme immunoassay and immunofluorescence analysis. We researched 968 children with allergic rhinitis, bronchial asthma, urticaria, and/or allergic dermatitis undergoing treatment at the Consultative and Diagnostic Center for Allergology and Immunology, GDKB No. 2 named after V.P. Bisyarina of Omsk. Blood serum samples were diagnosed in the laboratory of clinical immunology. Also, the allergen-specific IgE concentrations were determined for 109 allergens, including food allergens (milk, egg, meat, fish, cereals, fruits, vegetables), household allergens (epidermal pets, dust and house dust mites, molds), and inhaled antigens (grass, trees). Analysis of 435 children was carried out by enzyme immunoassay, and 533 children by indirect immunofluorescence. The research revealed the predominance in children with allergic diseases in Siberia of IgE-mediated sensitization to household allergens, which were detected in 33.7% of the examined. IgE-mediated sensitization to inhalation and food allergens was 1.5 times less common. According to our research, the most common causative allergen among households was house dust (90%), dandruff and animal epithelium (86%), house dust mites (13.5%), and molds (1.4%). The most common causative allergen among pollen was tree allergens (47.8%), cereals (23.5%) and weeds (20.1%). The presence of multivalent sensitization with a predominant combination of household and respiratory allergens and a rarer combination of household and food allergens has been experimentally established.
Full Text
##article.viewOnOriginalSite##About the authors
T. F. Sokolova
Omsk State Medical University
Email: eleon_74@mail.ru
PhD, MD (Medicine), Associate Professor, Department of Pharmacolody
Russian Federation, OmskE. L. Shcheglova
Omsk State Medical University
Author for correspondence.
Email: eleon_74@mail.ru
PhD (Medicine), Associate Professor, Department of Pathological Physiology
Russian Federation, OmskE. Yu. Sokolov
Karpovka Scientific and Clinical Center
Email: eleon_74@mail.ru
PhD (Medicine), Psychotherapist
Russian Federation, Saint PeterburgYu. V. Emelyanov
Pirogov Russian National Research Medical University
Email: eleon_74@mail.ru
PhD (Medicine), Associate Professor, Department of Psychotherapy, Institute of Clinical Psychology and Social Work
Russian Federation, MoscowReferences
- Ахапкина И.Г., Желтикова Т.М. Сравнительный анализ содержания клещей домашней пыли и их аллергенов в жилых помещениях г. Москвы // Иммунология, 2013. № 2. С. 108-111. [Akhapkina I.G., Zheltikova T.M. A comparative analysis of the content of house dust mites and their allergens in residential premises of Moscow. Immunologiya = Immunologiya, 2013, no. 2, pp. 108-111. (In Russ.)]
- Байшева М.Г., Дьячкова А.А., Прокопьева А.А., Хлопенкова О.В. Характеристика распространенности аллергических заболеваний и инвалидности больных бронхиальной астмой в республике Мордовия за период 2019-2021 г. // Клиническая больница, 2023. № 4 (39). С. 5-9. [Baysheva M.G., Dyachkova A.A., Prokopieva A.A., Khlopenkova O.V. Сharacteristics of allergic diseases and disability of patients of the prevalence with bronchial asthma in the republic of Mordovia for the period 2019-2021. Klinicheskaya bolnitsa = Clinical Hospital, 2023, no. 4 (39), pp. 5-9. (In Russ.)]
- Знаменская Л.К., Тришина С.В., Гостищева Е.В., Реган О.В. Опыт применения аллергенспецифической иммунотерапии (асит) препаратом антиполлин микст сорных трав-2 в лечении сезонного аллергического ринита, вызванного пыльцой сорных трав // Таврический медико-биологический вестник, 2023. Т. 26, № 1. С. 22-26. [Znamenskaya L.K., Trishina C.V., Gostishheva E.V., Regan O.V. Application experience allergen-specific immunotherapy (ASIT) with the drug antipollin mixed weed herbs-2 in the treatment of seasonal allergic rhinitis caused by weed pollen. Tavricheskiy mediko-biologicheskiy vestnik = Taurida of the Medical and Biological Bulletin, 2023, Vol. 26, no. 1, pp. 22-26. (In Russ.)]
- Мельникова К.С., Кувшинова Е.Д., Ревякина В.А. Аллергические заболевания в раннем возрасте // Педиатрия. Consilium Medicum, 2021. № 2. С. 141-145. [Melnikova K.S., Kuwshinowa E.D., Reviakina V.A. Allergic diseases at an early age. Pediatriya. Consilium Medicum = Pediatrics. Consilium Medicum, 2021, no. 2, pp. 141-145. (In Russ.)]
- Намазова-Баранова Л.С., Сновская М.А., Митюшин И.Л., Кожевникова О.В., Батырова А.С. Особенности диагностики аллергии у детей // Вестник РАМН, 2017. Т. 72, № 1. С. 33-41. [Namazova-Baranova L.S., Snovskaya M.A., Mityushin I.L., Kozhevnikova O.V., Batyrova A.S. Features of the diagnosis of allergies in children. Vestnik RAMN = Bulletin of RAMS, 2017, Vol. 72, no. 1, pp. 33-41. (In Russ.)]
- Пампура А.Н., Варламов Е.Е., Конюкова Н.Г. Сенсибилизация к аллергенам домашних животных // Российский вестник перинатологии и педиатрии, 2018. Т. 63, № 2. C. 22-26. [Pampura A.N., Varlamov E.E., Konyukova N.G. Sensitization to pet allergens. Rossiyskiy vestnik perinatologii i pediatrii = Russian Bulletin of Perinatology and Pediatrics, 2018, Vol. 63, no. 2, pp. 22-26. (In Russ.)]
- Петрова С.Ю., Халгатян С.В., Бержец В.М., Емельянова О.Ю. Десятилетнее наблюдение за сенсибилизацией к клещам домашней пыли // Журнал микробиологии, эпидемиологии и иммунобиологии, 2021. Т. 98: online-first. [Petrova S.Yu., Khlgatian S.V., Berzhets V.M., Emelyanova O.Yu. Ten-year monitoring of sensitization to house dust mites. Zhurnal mikrobiologii, epidemiologii i immunobiologii = Journal of Microbiology, Epidemiology and Immunobiology, 2021, Vol. 98: online-first. (In Russ.)] doi: 10.36233/0372-9311-49.
- Перевощикова Н.К., Гладков С.Ф., Черных Н.С. Возможности профилактики аллергических заболеваний с учетом региональных особенностей // Мать и дитя в Кузбассе, 2017. Т. 69, № 2. С. 38-46. [Perevoshchikova N.K., Gladkov S.F., Chernykh N.S. Possibilities for the prevention of allergic diseases, taking into account regional characteristics. Mat i ditya v Kuzbasse = Mother and Child in Kuzbass, 2017, Vol. 69, no. 2, pp. 38-46. (In Russ.)]
- Хабибулина Л.Р., Власова Н.В., Манжос М.В., Кавеленова Л.М. Анализ роли аллергокомпонентов в комплексной диагностике поллиноза и прогнозировании эффективности аллерген-специфической иммунотерапии // РМЖ, 2019. № 3. С. 7-10. [Khabibulina L.R., Vlasova N.V., Manzhos M.V., Kavelenova L.M. Blood analysis of allergic components in the comprehensive diagnosis of hay fever and predicting the effectiveness of allergen-specific immunotherapy. RMZh = Russian Medical Journal, 2019, no. 3, pp. 7-10. (In Russ.)]
- Asarnoj A., Hamsten C., Waden K., Lupinek C., Andersson N., Kull I., Curin M., Anto J., Bousquet J., Valenta R., Wickman M., van Hage M. Sensitization to cat and dog allergen molecules in childhood and prediction of symptoms of cat and dog allergy in adolescence: A BAMSE/MeDALL study. J. Allergy Clin. Immunol., 2016, Vol. 137, no 3, pp. 813-821.
- Konradsen J.R., Fujisawa T., van Hage M., Hedlin G., Hilger C., Kleine-Tebbe J., Matsui E.C., Roberts G., Rönmark E., Platts-Mills T.A.E. Allergy to furry animals: New insights, diagnostic approaches, and challenges. J. Allergy Clin. Immunol., 2015, Vol. 135, no. 3, pp. 616-625.
- Pyrhonen K., Nayha S., Laara E. Dog and cat exposure and respective pet allergy in early childhood. Pediatr. Allergy Immunol., 2015, Vol. 26, no. 3, pp. 247-255.
Supplementary files
