TLR-9 (-1237)*T/C polymorphism in russian COVID-19 patients from the chelyabinsk region
- Authors: Belyaeva S.V.1,2, Suslova T.A.1,2, Stashkevich D.C.1, Balandina S.E.1, mjakotina D.E.1, Milonchenko M.S.1
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Affiliations:
- Chelyabinsk State University
- Chelyabinsk Regional Blood Transfusion Station
- Issue: Vol 26, No 3 (2023)
- Pages: 217-222
- Section: SHORT COMMUNICATIONS
- URL: https://bakhtiniada.ru/1028-7221/article/view/253395
- DOI: https://doi.org/10.46235/1028-7221-9904-TPI
- ID: 253395
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Abstract
In COVID-19, the clinical outcome depends on a wide range of factors, including genetic features. Among them, TLRs, the genes encoding the receptors of innate immune system are of particular interest since they play the key role in development of innate immune response. The present study concerns the newely identified allelic variants of the TLR-9 (-1237)*T/C gene in Russian residents from the Chelyabinsk Region who had COVID-19 complicated by the bilateral viral pneumonia. Polymorphic variants of TLR-9 (-1237)*T/ C were determined by polymerase chain reaction. It was found that, among the COVID-19 patients, a TLR-9 allele (-1237 C) with higher transcriptional activity was more common than in the control group (19.421% and 11.275%, respectively, p = 0.019), and its homozygous genotype TLR-9 (-1237)*C was not detected in the comparison group. TLR-9 allele (-1237)*T in the patients with COVID-19 was less common in comparison with the control group (80.579% and 88.725%, respectively, p = 0.019). Taking into account the differences in suggested TLR-9 expression in more severe COVID-19 patients, we compared distribution of TLR-9 (-1237)*T/ С allele polymorphism in the patients with different severity of COVID-19. In the group of patients with mild form, the TLR-9 (-1237)*T/T genotype was more common as compared with patients who had more severe clinical course. The differences were significant at the trend level when compared with patients with a medium-severity disease (86.364% and 66,000%, respectively; p = 0.076).
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##article.viewOnOriginalSite##About the authors
Svetlana V. Belyaeva
Chelyabinsk State University; Chelyabinsk Regional Blood Transfusion Station
Author for correspondence.
Email: shshvetlana@yandex.ru
PhD (Biology), Biologist, Department of Laboratory Diagnostics, Laboratory for Diagnostics of Bloodborne Infections, associate Professor, Department of Microbiology, Immunology and General Biology, Faculty of Biology
Russian Federation, Chelyabinsk; ChelyabinskTatyana A. Suslova
Chelyabinsk State University; Chelyabinsk Regional Blood Transfusion Station
Email: hla_chel@mail.ru
PhD (Medicine), Head, Laboratory of Immunological Research, Chelyabinsk Regional Blood Transfusion Station; Associate Professor, Department of Microbiology, Immunology and General Biology, Faculty of Biology
Russian Federation, Chelyabinsk; ChelyabinskDaria C. Stashkevich
Chelyabinsk State University
Email: stashkevich_dary@mail.ru
PhD (Biology), Associate Professor, Dean, Faculty of Biology
Russian Federation, ChelyabinskSvetlana E. Balandina
Chelyabinsk State University
Email: lana.balandina.97@mail.ru
Assistant Professor, Postgraduate Student, Department of Microbiology, Immunology and General Biology, Faculty of Biology
Russian Federation, ChelyabinskDaria E. mjakotina
Chelyabinsk State University
Email: daha1607@mail.ru
Master Student, Faculty of Biology
Russian Federation, ChelyabinskMaria S. Milonchenko
Chelyabinsk State University
Email: rinarom5@gmail.com
Bachelor, Faculty of Biology
Russian Federation, ChelyabinskReferences
- Ковальчук Л.В., Свитич О.А., Ганковская Л.В., Мироншиченкова А.М., Ганковский В.А. Роль Toll-подобных рецепторов в патогенезе инфекционных заболеваний человека // Человек и его здоровье, 2012. № 2. С. 147-153. [Kovalchuk L.V., Svitich O.A., Gankovskaya L.V., Mironshichenkova A.M., Gankovskii, V.A. The role of Toll-like receptors in the pathogenesis of human infectious diseases. Chelovek i ego zdorovye = Man and His Health, 2012, no. 2, pp. 147-153. (In Russ.)]
- Щелканов М.Ю., Колобухина Л.В., Бургасова О.А., Кружкова И.С., Малеев В.В. COVID-19: этиология, клиника, лечение // Инфекция и иммунитет, 2020. № 10(3). С. 421-445. [Shchelkanov M.Yu., Kolobukhina L.V., Burgasova O.A., Kruzhkova I.S., Maleev V.V. COVID-19: etiology, clinic, treatment. Infektsiya i immunitet = Russian Journal of Infection and Immunity, 2020, no. 10 (3), pp. 421-445. (In Russ.)] doi: 10.15789/2220-7619-CEC-1473.
- Alhabibi A.M., Hassan A.S., Elbaky N.M., Eid H.A. Impact of Toll-Like Receptor 2 and 9 Gene Polymorphisms on COVID-19: Susceptibility, Severity, and Thrombosis. J. Inflamm. Res., 2023, no. 16, pp. 665-675.
- Al-Khatib S., Shabaneh A.M., Abdo N., Al-Eitan L. Association of TLR9-1237T>C; rs5743836 polymorphism with increased risk of Hodgkin’s lymphoma: A case-control study. PLoS One, 2022, Vol. 17, no. 7, e0272312. doi: 10.1371/journal.pone.0272312.
- Bezemer G., Garssen J. TLR9 and COVID-19: A multidisciplinary theory of a multifaceted therapeutic target. Front. Pharmacol., 2021, Vol. 11, 601685. doi: 10.3389/fphar.2020.601685.
- Costa T.J., Potje S.R., Fraga-Silva T.F.C., da Silva-Neto J.A., Barros P.R., Rodrigues D., Machado M.R., Martins R.B., Santos-Eichler R.A., Benatti M.N., de Sá K.S.G., Almado C.E.L., Castro Í.A., Pontelli M.C., La Serra L., Carneiro F.S., Becari C., Louzada-Junior P., Oliveira R.D.R., Zamboni D.S., Arruda E., Auxiliadora-Martins M., Giachini F.R.C., Bonato V.L.D., Zachara N.E., Bomfim G.F., Tostes R.C. Mitochondrial DNA and TLR9 activation contribute to SARS-CoV-2-induced endothelial cell damage. Vascul. Pharmacol., 2022, Vol. 142, 106946. doi: 10.1016/j.vph.2021.106946.
- Greene J.A., Moormann A.M., Vulule J., Bockarie M.J., Zimmerman P.A., Kazura J.W. Toll-like receptor polymorphisms in malaria-endemic population. Malar. J., 2009, Vol. 8, 50. doi: 10.1186/1475-2875-8-50.
- Gusev E., Sarapultsev A., Solomatina L., Chereshnev V. SARS-CoV-2-specific immune response and the pathogenesis of COVID-19. Int. J. Mol. Sci., 2022, Vol. 23, no. 3, 1716. doi: 10.3390/ijms23031716.
- Yang H.-Y., Lu K.-C., Lee H.-S., Huang S.-M., Lin Y.-F., Wu C.-C., Salter D.M., Su S.-L. Role of the functional Toll-like receptor-9 promoter polymorphism (-1237T/C) in increased risk of end-stage renal disease: A case-control study. PLoS One, 2013, Vol. 8, no. 3, e58444. doi: 10.1371/journal.pone.0058444.
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