EFFECT OF TRANSPLANTATION OF MESENCHIMAL STEM CELLS (MSC) AND PRODUCED MICROVESICLES (MV) ON THE MAINTENANCE OF MEMORY T-CELLS AND T-REGULATORY CELLS IN THE MODEL ACUTE RENAL FAILURE IN MICE
- Authors: Khabalova T.S.1, Kaschenko E.A.1, Seledtsova G.V.1
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Affiliations:
- Federal State Budget Scientific Institution “Scientific Research Institute of fundamental and clinical immunology” (SRIFCI)
- Issue: Vol 22, No 2-2 (2019)
- Pages: 957-959
- Section: ORIGINAL ARTICLES
- URL: https://bakhtiniada.ru/1028-7221/article/view/120030
- DOI: https://doi.org/10.31857/S102872210006505-0
- ID: 120030
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Abstract
MV has been shown to have a positive effect on the restoration of renal excretory function at a level comparable to the effect of MSCs. The use of MSC and MB in the treatment of acute renal failure resulted in almost the same result: on the 4th day the level of urea was 9.3 μg/ml and 9.37 μg/ml, respectively, which is 25% lower than in the glycerol control. By the 8th day, the level of urea was 7.9 μg/ ml after the introduction of MSC and 7.6 μg/ml after the introduction of MB, which is 21% lower than in the glycerol control. It has been shown that the MW produced by MSCs stimulate the proliferation of both splenocytes and cells of the kidney tissue in a dose-dependent manner. To study the resistance to apoptosis, splenocytes were used both from intact mice and from glycerol-induced ARF. The addition of MB causes a decrease in the level of apoptosis in both intact mice (21.73%) and mice with ARF (13.28%). Accordingly, inversely, the percentage of cells in the S / M phase of the cell cycle increased.
About the authors
T. S. Khabalova
Federal State Budget Scientific Institution “Scientific Research Institute of fundamental and clinical immunology” (SRIFCI)
Author for correspondence.
Email: khabalovat@gmail.com
Junior Reseacher, Laboratory of Molecular Mechanisms Head of the Laboratory of Cell Biotechnology,
Novosibirsk
Russian FederationE. A. Kaschenko
Federal State Budget Scientific Institution “Scientific Research Institute of fundamental and clinical immunology” (SRIFCI)
Email: fake@neicon.ru
Researcher, Laboratory of Molecular Mechanisms Head of the Laboratory of Cell Biotechnology,
Novosibirsk
Russian FederationG. V. Seledtsova
Federal State Budget Scientific Institution “Scientific Research Institute of fundamental and clinical immunology” (SRIFCI)
Email: niiki01@online.nsk.su
PhD, Head, Leading Reseacher of Cell Biotechnology Laboratory,
Novosibirsk
Russian FederationReferences
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