Peripheral blood cytotoxic T cells in patients with sarcoidosis
- Authors: Lazareva N..1,2, Kudryavtsev I..1,3, Baranova O..1, Serebriakova M..3, Ses’ T..1,2, Ilkovich M..1, Totolyan A..1,4
-
Affiliations:
- Academician I.P. Pavlov First St. Petersburg State Medical University
- State Research Institute of Highly Pure Biopreparations
- Institute of Experimental Medicine
- St. Petersburg Pasteur Institute
- Issue: Vol 21, No 3 (2018)
- Pages: 348-353
- Section: ORIGINAL ARTICLES
- URL: https://bakhtiniada.ru/1028-7221/article/view/119884
- DOI: https://doi.org/10.31857/S102872210002408-3
- ID: 119884
Cite item
Full Text
Abstract
Based on CD45RA, CD62L, CD27 и CD28 expression several subsets of peripheral blood CD3+CD8+ lymphocytes were measured in patients with chronic sarcoidosis (n=46). The percentage of “non-eff ector” CD3+CD8+ subsets – “naïve” and central memory – were decreased in comparison with healthy control group (n=46), whereas the relative number of TEMRA cells was signifi cantly increased. Within EM subset percentage of EM1 cells was lower in sarcoidosis group, while among TEMRA the relative number of рЕ2 and mature eff ector cells were increased if compared with healthy control group. Thereby, our results indicate the intensifi cation of CD3+CD8+ lymphocytes maturation in the lymphoid tissue under a constant antigenic stimulation.
About the authors
N. . Lazareva
Academician I.P. Pavlov First St. Petersburg State Medical University; State Research Institute of Highly Pure Biopreparations
Author for correspondence.
Email: noemail@neicon.ru
Russian Federation
I. . Kudryavtsev
Academician I.P. Pavlov First St. Petersburg State Medical University; Institute of Experimental Medicine
Email: noemail@neicon.ru
Russian Federation
O. . Baranova
Academician I.P. Pavlov First St. Petersburg State Medical University
Email: noemail@neicon.ru
Russian Federation
M. . Serebriakova
Institute of Experimental Medicine
Email: noemail@neicon.ru
Russian Federation
T. . Ses’
Academician I.P. Pavlov First St. Petersburg State Medical University; State Research Institute of Highly Pure Biopreparations
Email: noemail@neicon.ru
Russian Federation
M. . Ilkovich
Academician I.P. Pavlov First St. Petersburg State Medical University
Email: noemail@neicon.ru
Russian Federation
A. . Totolyan
Academician I.P. Pavlov First St. Petersburg State Medical University; St. Petersburg Pasteur Institute
Email: noemail@neicon.ru
Russian Federation
References
- Визель А. А., Визель И. Ю., Амиров Н. Б. Эпидемиология саркоидоза в Российской Федерации. Вестник современной клинической медицины, 2017, 10 (5), 66–73. [Vizel A. A., Vizel I. Yu., Amirov N. B. Epidemiology of sarcoidosis in the Russian Federation. Bulletin of Contemporary Clinical Medicine, 2017, 10 (5), 66–73].
- Sakthivel P., Bruder D. Mechanism of granuloma formation in sarcoidosis. Curr. Opin. Hematol. 2017, 24(1), 59–65.
- Shigehara K., Sh? ubo N., Ohmichi M., Takahashi R., Kon S., Okamura H., Kurimoto M., Hiraga Y., Tatsuno T., Abe S., Sato N. IL-12 and IL-18 are increased and stimulate IFN-gamma production in sarcoid lungs. J. Immunol. 2001, 166 (1), 642–649.
- Georas S. N., Chapman T. J., Crouser E. D. Sarcoidosis and T-helper cells. Th1, Th17, or Th17.1? Am. J. Respir. Crit. Care Med. 2016, 193 (11), 1198–200.
- D’Errico S., Bello S., Cantatore S., Neri M., Riezzo I., Turillazzi E., Fineschi V. Immunohistochemical characterisation and TNF-? expression of the granulomatous infi ltration of the brainstem in a case of sudden death due to neurosarcoidosis, Case report. Forensic Science International 2011, 208, e1–e5.
- Katchar K., Soderstrom K., Wahlstrom J., Eklund A., Grunewald J. Characterization of natural killer cells and CD56+ T-cells in sarcoidosis patients. Eur. Respir. J. 2005, 26(1), 77–85.
- Хайдуков С. В., Байдун Л. А., Зурочка А. В., Тотолян А. А. Стандартизованная технология «исследование субпопуляционного состава лимфоцитов периферической крови с применением проточных цитофлюориметров-анализаторов» (проект). Медицинская иммунология 2012, 14(3), 255–268. [Khaydukov S., Baidun L., Zurochka A., Totolyan A. Methods. Medical Immunology (Russia) 2012, 14(3), 255–268. (In Russ.)].
- Кудрявцев И. В., Борисов А. Г., Волков А. Е., Савченко А. А., Серебрякова М. К., Полевщиков А. В. Анализ уровня экспрессии CD56 и CD57 цитотоксическими Т-лимфоцитами различного уровня дифференцировки. Тихоокеанский медицинский журнал 2015, 2(60), 30–35. [Kudryavtsev I. V., Borisov A. G., Volkov A. E., Savchenko A. A., Serebryakova M. K., Polevschikov A. V. CD56 and CD57 expression by distinct populations of human cytotoxic T lymphocytes. Pacifi c Medical Journal 2015, 2(60), 30–35].
- Кудрявцев И. В., Борисов А. Г., Кробинец И. И., Савченко А. А., Серебрякова М. К. Определение основных субпопуляций цитотоксических Т-лим фо цитов методом многоцветной проточной цитометрии. Медицинская иммунология 2015, 17(6), 525–538. [Kudryavtsev I. V., Borisov A. G., Krobinets I. I., Savchenko A. A., Serebryakova M. K. Multicolor fl ow cytometric analysis of cytotoxic T cell subsets. Medical Immunology (Russia) 2015, 17(6), 525–538.].
- Parasa V. R., Forsslund H., Enger T., Lorenz D., Kullberg S., Eklund A., SkoldvM., Wahlstrom J., Grunewald J., Brighenti S. Enhanced CD8+ cytolytic T cell responses in the peripheral circulation of patients with sarcoidosis and non-Lofgren’s disease. Respir Med. 2018, 138, 38–44.
- Ding J., Dai J., Cai H., Gao Q., Wen Y. Extensively disturbance of regulatory T cells – Th17 cells balance in stage II pulmonary sarcoidosis. Int J Med Sci. 2017, 14(11), 1136–1142.
- Braun N. A., Celada L. J., Herazo-Maya J. D., Abraham S., Shaginurova G., Sevin C.M., Grutters J., Culver D. A., Dworski R., Sheller J., Massion P. P., Polosukhin V. V., Johnson J. E., Kaminski N., Wilkes D. S., Oswald-Richter K. A., Drake W. P. Blockade of the programmed death-1 pathway restores sarcoidosis CD4(+) T-cell proliferative capacity. Am J Respir Crit Care Med. 2014, 190(5), 560–571.
- Wahlstrom J., Katchar K., Wigzell H., Olerup O., Eklund A., Grunewald J. Analysis of intracellular cytokines in CD4+ and CD8+ lung and blood T cells in sarcoidosis. Am J Respir Crit Care Med. 2001, 163(1), 115–121.
- Кудрявцев И. В. Т-клетки памяти: основные популяции и стадии дифференцировки. Российский иммунологический журнал. 2014, 8, 4 (17), 947– 964. [Kudryavtsev I. V. Memory T cells: major populations and stages of diff erentiation. Russian Journal of Immunology. 2014, 8, 4 (17), 947–964].
- Romero P., Zippelius A., Kurth I., Pittet M.J., Touvrey C., Iancu E.M., Corthesy P., Devevre E., Speiser D.E., Rufer N. Four functionally distinct populations of human eff ector-memory CD8+ T lymphocytes. J Immunol. 2007, 178(7), 4112–4129.
Supplementary files
