MORPHOLOGICAL AND FUNCTIONAL DIVERSITY OF MACROPHAGES OF DIFFERENT LOCALIZATION IN ANIMALS IN NORMAL AND IN CONDITIONS OF DIABETES MELLITUS TYPE I
- Authors: Pozdina V.A.1,2, Danilova I.G.1
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Affiliations:
- Institute of Immunology and Physiology, Ural Branch of the Russian Academy of Sciences
- Ural Scientific Research Institute of Phthisiopulmonology – branch of FGBU “NMIc FPI”, Ministry of Health of the Russian Federation
- Issue: Vol 22, No 2-2 (2019)
- Pages: 894-896
- Section: ORIGINAL ARTICLES
- URL: https://bakhtiniada.ru/1028-7221/article/view/119853
- DOI: https://doi.org/10.31857/S102872210006571-3
- ID: 119853
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Abstract
The study investigated the morphological and functional characteristics of macrophages of different localization, isolated from intact animals and animals with a model of diabetes mellitus type I. The study was performed on rat macrophage cultures isolated from the following areas: lungs, spleen, liver, and peritoneal cavity. The following morphometric parameters were determined: S cells, cytoplasm and nuclei, nuclear-cytoplasmic ratio (NCO). The phenotype was determined by the expression of CD163 and CD80. The macrophage cytokine activity was assessed by the level of IL-1α, IL-10 and TNFα. The morphological characteristics of macrophages from various organs were demonstrated and their functional differences were revealed.
About the authors
V. A. Pozdina
Institute of Immunology and Physiology, Ural Branch of the Russian Academy of Sciences;Ural Scientific Research Institute of Phthisiopulmonology – branch of FGBU “NMIc FPI”, Ministry of Health of the Russian Federation
Author for correspondence.
Email: varya.pozdina@inbox.ru
JR, Laboratory of Morphology and Biochemistry;
JR, Laboratory of Biomedical Research,
Ekaterinburg
Russian FederationI. G. Danilova
Institute of Immunology and Physiology, Ural Branch of the Russian Academy of Sciences
Doctor of Biological Sciences, Head of the Laboratory of Morphology and Biochemistry,
Ekaterinburg
Russian FederationReferences
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