BIOLUMINESCENT CHEMILUMINESCENT MARKERS FOR ASSESSMENT OF PHYSICAL STATUS OF ATHLETES FOR DETERMINATION PERFORMANCE LEVEL

The aim of this study was to determine the activity bioluminescent chemiluminescent markers of saliva, serum and blood phagocytes as markers of the physical status of athletes. 2 groups of athletes, with the prevailing aerobic (skiers and ice-skaters, n=16) and anaerobic (sliders and bobsled racers, n=16) types of exercise, aged from 16 to 30 years old, were selected. Studies were carried out under the PWC170 testing. Saliva and blood samples were collected before and under W1, W2, PWC170 types of loads. The determination of activity of pro- and antioxidants was registered using the chemiluminescent method. The determination of the physiologic status was registered using the bioluminescent method. The imbalance in the activity of pro-oxy- and antioxidants was detected for athletes with prevailing aerobic loads. For these athletes were measured increased production of pro-oxidants and decreased the production of antioxidants. In addition, irreversible changes, inhibition of the activity of antioxidant mechanisms, and increased production of reactive oxygen intermediates were received. The opposite situation is observed in athletes with a predominant anaerobic load. Results of bioluminescent bioassay show signifi cant differences between athletes with prevailing aerobic and anaerobic types of loads, as well as with diff erent sports qualifications.

chemiluminescence, bioluminescence, physical status, performance