VARIOUS APPLICATIONS OF A METHOD OF A RESEARCH OF LEVELS OF CYTOKIN OF NEUTROPHILS

In a research approaches to assessment of levels of cytokin of neutrophils on the basis of secretion by neutrophils the preforming of cytokins after an hour incubation are offered the RPMI 1640 environment. Secretion of cytokins was defined with use of multiplex sets of the Biorad company (USA), Luminex (USA), for definition of various cytokins on the MAGPIX-100 device (USA) and the immunofermental analysis (IFA). It is established that at an incubation of neutrophils in the environment of RPMI 1640 within 1 hour in the environment of an incubation (supernatant) various cytokins come to light concentration of which in a supenatant of neutrophils exceeded reference level (RPMI-1640 environment). At addition to neutrophils of various bacteria, supernatant of bacteria, regulatory molecules, the activity of secretion changes (is stimulated or supressed) depending on that substrate which was added to the environment of an incubation of live neutrophils. Thus, the offered methodical approach of assessment of levels of cytokin of neutrophils allows to study mechanisms of regulation of cytokins network of these cages.

neutrophils, cytokins, bacteria, peptides