Monitoring of rabies in wild animals in the Kirov region after oral immunization
- Authors: Zaykova O.N.1, Grebennikova T.V.1, Elakov A.L.1, Kochergin-Nikitsky K.S.1, Aliper T.I.1, Chuchalin S.F.2, Gulyukin A.M.3
-
Affiliations:
- «Federal Research Centre of Epidemilogy and Microbiology named after the honorary academician N.F. Gamaleya»
- The Veterinary Department of the Kirov Region
- Kovalenko All-Russian Scientific Research Institute of Experimental Veterinary
- Issue: Vol 61, No 4 (2016)
- Pages: 186-192
- Section: ORIGINAL RESEARCHES
- URL: https://bakhtiniada.ru/0507-4088/article/view/117906
- DOI: https://doi.org/10.18821/0507-4088-2016-61-4-186-192
- ID: 117906
Cite item
Full Text
Abstract
This work presents the results of the molecular genetic research on genomes of field isolates of the rabies virus circulating in the territory of the Kirov region in order to analyze the phylogenetic relationship between the wild isolate genomes and to determine the possible reversion of the vaccine strain of the rabies virus used in the oral vaccine to virulent variant. We studied 24 brain samples from wild carnivores shot after oral immunization of the area with Rabivak-O/333. A bait with the vaccine provided by the Veterinary Service of the Kirov was also studied. All samples were found to be positive for the presence of the rabies virus as established by FAT and RT-PCR techniques. Phylogenetic analysis of N genome fragments of the rabies virus showed that the field isolates from the Kirov regions were genetically close to the field isolates from Buryatia 2012. Analysis of G genome fragments showed that the Kirov field isolates were close to the isolates from Lipetsk (2011), as well as to the Ukrainian isolates (2006 and 2010). Molecular genetic analysis of the gene fragments N and G for the field isolates and fragments of the genome of the rabies virus vaccine did not reveal any reversion to the virulent vaccine strain.
About the authors
O. N. Zaykova
«Federal Research Centre of Epidemilogy and Microbiology named after the honorary academician N.F. Gamaleya»
Author for correspondence.
Russian Federation
T. V. Grebennikova
«Federal Research Centre of Epidemilogy and Microbiology named after the honorary academician N.F. Gamaleya»
Email: t_grebennikova@mail.ru
Russian Federation
A. L. Elakov
«Federal Research Centre of Epidemilogy and Microbiology named after the honorary academician N.F. Gamaleya»Russian Federation
K. S. Kochergin-Nikitsky
«Federal Research Centre of Epidemilogy and Microbiology named after the honorary academician N.F. Gamaleya»Russian Federation
T. I. Aliper
«Federal Research Centre of Epidemilogy and Microbiology named after the honorary academician N.F. Gamaleya»Russian Federation
S. F. Chuchalin
The Veterinary Department of the Kirov RegionRussian Federation
A. M. Gulyukin
Kovalenko All-Russian Scientific Research Institute of Experimental VeterinaryRussian Federation
References
- Федеральная служба по ветеринарному и фитосанитарному надзору. Available at: www.fsvps.ru.
- Всемирная организация здравоохранения. Available at: http://www.who.int/ru.
- Львов Д.К., ред. Руководство по вирусологии: Вирусы и вирусные инфекции человека и животных. М.: ООО «Издательство «Медицинское информационное агентство»; 2013.
- Васильев Д.А., Луговцев В. Ю., ред. Вирусы вызывающие болезни общие для многих видов сельскохозяйственных животных. Курс лекций по вирусологии. Часть вторая. Ульяновск; 2004.
- Сливко И.А., Сафонов Г.А., Хрипунов Е.М., Гогин А.Е., Жестерев В.И., Баньковский Д.О. Возможные причины неудачи оральной антирабической иммунизации диких плотоядных животных в Российской Федерации. Ветеринария. 2013; (10): 27-31.
- Шабейкин А.А., Гулюкин А.М., Цареградский П.Ю., Паршикова А.В., Южаков А.Г., Зайкова О.Н. Анализ текущей эпизоотической ситуации по бешенству на территории Российской федерации. Российский ветеринарный журнал. Сельскохозяйственные животные. 2015; (4): 5-8.
- Баньковский Д.О. Иммунобиологические свойства штамма ERAG 333 вируса бешенства для изготовления оральной антирабической вакцины: Дисс. … канд. вет. наук. Щелково; 2010.
- Елаков А.Л., Зайкова О.Н., Кочергин-Никитский К.С., Гребенникова Т.В., Алипер Т.И. Мониторинг бешенства у диких животных в Брянской области. Ветеринария. 2015; (1): 11-4.
- Метлин А.Е. Молекулярно-биологические характеристики полевых изолятов и аттенуированных штаммов вируса бешенства: Дисс. … канд. вет. наук. Владимир; 2004.
- Чупин С.А., Чернышова Е.В., Метлин А.Е. Генетическая характеристика полевых изолятов вируса бешенства, выявленных на территории Российской Федерации в период 2008-2011 гг. Вопросы вирусологии. 2013; (4): 44-9.
- Fehlner-Gardiner C., Nardine-Davis S., Armstrong J., Muldoon F., Bachmann P., Wandeler A. Era vaccine-derived cases of rabies in wildlife and domestic animals in Ontario, Canada, 1989-2004. J. Wildl. Dis. 2008; 44 (1): 71-85.
- Müller T., Bätza H.J., Beckert A., Bunzenthal C., Cox J.H., Freuling C.M. et al. Analysis of vaccine-virus-associated rabies cases in red foxes (Vulpesvulpes) after oral rabies vaccination campaigns in Germany and Austria. Arch. Virol. 2009; 154 (7): 1081-91.
- Metlin A.E., Rybakov S., Gruzdev K., Neuvonen E., Huovilainen A. Genetic heterogeneity of Russian, Estonian and Finnish field rabies viruses. Arch. Virol. 2007; 152 (9): 1645-54.
- Pant G.R., Lavenir R., Wong F.Y., Certoma A., Larrous F., Bhatta D.R. et al. Recent emergence and spread of an arctic-related phylogenetic lineage of rabies virus in Nepal. PLoS Negl. Trop. Dis. 2013; 7 (11): e2560.
- Metlin A., Paulin L., Suomalainen S., Neuvonen E., Rybakov S., Mikhalishin V. et al. Characterization of Russian rabies virus vaccine strain RV-97. Virus Res. 2008; 132 (1-2): 242-7.
Supplementary files
