Мақаланы E-mail арқылы жіберу UV-Visible Light-Induced Luminescence Processes in Non-Aromatic Amino Acids Solution at Room Temperature
- Авторлар: Terpugov E.L1, Udaltsov S.N2, Degtyareva O.V1
-
Мекемелер:
- Institute of Cell Biophysics, Russian Academy of Sciences
- Institute of Physicochemical and Biological Problems in Soil Science, Russian Academy of Sciences
- Шығарылым: Том 70, № 1 (2025)
- Беттер: 14-21
- Бөлім: Molecular biophysics
- URL: https://bakhtiniada.ru/0006-3029/article/view/285378
- DOI: https://doi.org/10.31857/S0006302925010029
- EDN: https://elibrary.ru/LXZNDY
- ID: 285378
Дәйексөз келтіру
Ашық рұқсат
Рұқсат берілді
Тек жазылушылар үшін
Аннотация
We demonstrate for the first time that aqueous solutions of non-aromatic amino acids such as L-arginine hydrochloride, L-lysine hydrochloride and glycine can simultaneously emit fluorescence and afterglow upon excitation with UV-visible light at room temperature. The luminescence afterglow differs from conventional fluorescence by its weak intensity and long emission duration. The presence of short-lived and long-lived fluorescence in non-traditional luminophores indicates a dual nature of fluorescence and the existence of excited states of different natures. The detected correlation in the shape of short-lived and long-lived fluorescence spectra suggests that the luminescence afterglow corresponds to thermally activated delayed fluorescence arising through the mechanism of reconversion from the lowest triplet state T1 to the lowest singlet state S1. Further studies will help to shed light on the understanding of the biophysics of photoinduced processes in biological systems.
Авторлар туралы
E. Terpugov
Institute of Cell Biophysics, Russian Academy of SciencesPushchino, Russia
S. Udaltsov
Institute of Physicochemical and Biological Problems in Soil Science, Russian Academy of SciencesPushchino, Russia
O. Degtyareva
Institute of Cell Biophysics, Russian Academy of Sciences
Email: olga_degt@mail.ru
Pushchino, Russia
Әдебиет тізімі
- Shukla A., Mukherjee S., Sharma S., Agrawal V., Kishan K. V. R., and Guptasarma P. A novel UV laser induced visible blue radiation from protein crystals and aggregates: scattering artifacts or fluorescence transitions of peptide electrons delocalized through hydrogen bonding? Arch. Biochem. Biophys., 428 (2), 144–153 (2004). doi: 10.1016/j.abb.2004.05.007
- Chen X., Luo W., Ma H., Peng Q., Yuan W. Z., and Zhang Y. Prevalent intrinsic emission from nonaromatic amino acids and (poly(aminoacids). Sci. China Chen., 61 (3), 351–359 (2018). doi: 10.1007/s11426-017-9114-4
- Homchaudhuri L. and Swaminathan R. Novel absorption and fluorescence characteristics of L-lysine. Chem. Lett., 30 (8), 844−845 (2001).
- del Mercato L. L., Pompa P. P., Maruccio G., Torre A. D., Sabella S., Tamburro A. M., Cigolani R., and Rinaldi R. Charge transport and intrinsic fluorescence in amyloid-like fibrils. Proc. Natl. Acad. Sci. USA., 104 (46), 18019–18024 (2007). doi: 10.1073/pnas.0702843104
- Chan F. T. S., Kaminski Schierle G. S., Kumita J. R., Bertoncini C. W., Dobson C. M. and Kaminski C. F. Protein amyloids develop an intrinsic fluorescence signature during aggregation. Analyst., 138 (7), 2156–2162 (2013). doi: 10.1039/C3AN36798C
- Zhang H., Zhao Z., McGonigal P. R., Ye R., Liu S., Lam J. W. Y., Kwok R. T. K., Yuan W. Z., Xie J., Rogach A. L., and Tang B. Z. Clusterization-triggered emission: Uncommon luminescence from common materials. Materials Today, 32, 275–292 (2020). doi: 10.1016/j.mattod.2019.08.010
- Guptasarma P. Solution-state characteristics of the ultraviolet A-induced visible fluorescence from proteins. Arch. Biochem. Biophys., 478 (2), 127–129 (2008). doi: 10.1016/j.abb.2008.08.002
- Ye R., Liu Y., Zhang H., Su H., Zhang Y., Xu L., Hu R., Kwok R. T. K., Wong K. S., Lam J. W. Y., Goddard III W. A., and Tang B. Z. Non-conventional fluorescent biogenic and synthetic polymers without aromatic rings. Polym. Chem., 8 (10), 1722–1727 (2017). doi: 10.1039/c7py00154a
- Pinotsi D., Buell A. K., Dobson C. M., Kaminski Schierle G. S., and Kaminski C. F. A label-free, quantitative assay of amyloid fibril growth based on intrinsic fluorescence. Chem. Bio. Chem., 14 (7), 846–850 (2013). doi: 10.1002/cbic.201300103
- Hong Y., Lam J. W. Y., and Tang B. Z. Aggregation-induced emission: Phenomenon, mechanism and applications. Chem. Commun., 29, 4332–4353 (2009). doi: 10.1039/B904665H
- Homchaudhuri L. and Swaminathan A.G. Near ultraviolet absorption arising from lysine residues in close proximity: a probe to monitor protein unfolding and aggregation in lysine-rich proteins. Bull. Chem. Soc. Jpn., 77 (4), 765–766 (2004). doi: 10.1246/bcsj.77.765
- Liao P., Huang J., Yan Y., and Tang B. Z. Clusterizationtriggered emission (CTE): one for all, all for one. Mater. Chem. Front., 5 (18), 6693−6717 (2021). doi: 10.1039/D1QM00808K
- Pinotsi D., Grisanti L., Mahou P., Gebauer R., Kaminski C.F., Hassanali A., and Kaminski Schierle G. S. Proton transfer and structure-specific fluorescence in hydrogen bond-rich protein structures. J. Am. Chem. Soc., 138 (9), 3046–3057 (2016). doi: 10.1021/jacs.5b11012
- Zhao W., He Z., and Tang B. Z. Room-temperature phosphorescence from organic aggregates. Nat. Rev. Mater., 5 (12), 869–885 (2020). doi: 10.1038/s41578-020-0223-z
- Теренин А. Н. Фотоника молекул красителей и родственных органических соединений (Наука, Ленинград, 1967).
- Lakowicz J. R. Principles of Fluorescence Spectroscopy, 3rd edition, Ed. by J. R. Lakowicz (Springer, Berlin., Germany, 2006).
- Luo X., Tian B., Zhai Y., Guo H., Liu S., Li J., Li S., James T. D., and Chen Z. Room-temperature phosphorescent materials derived from natural resources. Nat. Rev. Chem., 7 (11), 800–812 (2023). doi: 10.1038/s41570-023-00536-4
- Дегтярева О. В., Афанасьев В. Н., Хечинашвили Н. Н., Терпугов Е. Л. Структура и свойства L-лизина монохлорида и L-глицина в жидкой фазе при воздействии оптическим излучением низкой интенсивности. Современные проблемы науки и образования, 4, 288 (2013). URL: https://scienceeducation.ru/ru/article/view?id=10010.
- Terpugov E. L., Kondratyev M. S. and Degtyareva O. V. Light-induced effects in glycine aqueous solution studied by Fourier transform infrared-emission spectroscopy and ultraviolet-visible spectroscopy. J. Biomol. Struct. Dynamics, 39 (1), 108−117 (2020). doi: 10.1080/07391102.2020.1717628
- Terpugov E. L., Udaltsov S. N., and Degtyareva O. V. Study of the Spectral Characteristics of L-Lysine and L-Arginine Using UV-VIS Spectroscopy and SteadyState and Synchronous Fluorescence Spectroscopy. Biophysics, 66 (5), 726–732 (2021). doi: 10.1134/S0006350921050250
- Demchenko A. P. The red-edge effects: 30 years of exploration. Luminescence, 17 (1), 19–42 (2002). doi: 10.1002/bio.671
- Борн М. и Вольф Э. М. Основы оптики (Наука, М., 1973).
- Khanarian G. and Moore W. J. The Kerr effect of amino acids in water. Austral. J. Chem., 33 (8), 1727–1741 (1980). doi: 10.1071/CH9801727
- Dou X., Zhou Q., Chen X., Tan Y., He X., Lu P., Sui K., Tang B. Z., Zhang Y., and Yuan W. Z. Clustering-triggered emission and persistent room temperature phosphorescence of sodium alginate. Biomacromolecules, 19 (6), 2014−2022 (2018). DOI: doi.org/10.1021/acs.biomac.8b00123
- Xiao G., Fang X., Ma Yu-J., and Yan D. Multi-mode and dynamic persistent luminescence from metal cytosine halides through balancing excited-state proton transfer. Adv.Sci. (Weinh), 9 (16), e2200992 (2022). doi: 10.1002/advs.202200992
- Zhang P., Zhang S., Yang J., Li P., and Li H. Clusterization-triggered room temperature phosphorescence supramolecular assembly with extraordinary stimulus-responsive features from nonaromatic amino acids. Chemistry – Eur. J., 29 (38), e202300371 (2023). doi: 10.1002/chem.202300371
Қосымша файлдар
